RHCE*ceCF encodes partial c and partial e but not CELO, an antigen antithetical to Crawford

Abstract

Background RH43 (Crawford) is encoded by RHCE*ce with nucleotide changes 48G>C, 697C>G, and 733C>G (RHCE*ceCF). We investigated the Rh antigen expression and antibody specificities in four patients with this allele. Methods Hemagglutination tests, DNA extraction, PCR-RFLP, AS-PCR, reticulocyte RNA isolation, RT-PCR cDNA analyses, cloning, and sequencing were performed by standard procedures. Results RBCs from two patients typed D+C−E−c+e+/−, hrS−/+W, hrB− and their serum was reactive (3+) with all RBC samples of common Rh phenotype tested, but non reactive with Rhnull or D- - RBCs (apparent alloanti-Rh17). At the RHCE locus, Patient 1 was homozygous for RHCE*ceCF, and Patient 2 inherited RHCE*ceCF in trans to a silenced RHCE*cE. Cross testing of serum and RBCs from these two samples showed mutual compatibility, indicating that both antibodies define the same novel high prevalence antigen on Rhce. Two additional patients, one whose serum contained alloanti-c but the RBCs typed C+c+, and one whose serum contained anti-e but the RBCs typed E+e+, also had RHCE*ceCF. RHCE*Ce was present in trans in the former and RHCE*cE in the latter patient. Conclusion We report that amino acid changes on RhceCF (Trp16Cys, Gln233Glu, and Leu245Val) alter the protein to the extent that c and e antigens are partial, and a high prevalence antigen, we have named CELO (provisional ISBT number 004058; RH58) is not expressed. CELO is antithetical to RH43 (Crawford).

RHCE*ceMO is frequently in cis to RHD*DAU0 and encodes a hrS–, hrB–, RH:–61 phenotype in black persons: clinical significance

“…Alternatively, reverse transcription was carried out with RHD and RHCE -specific primers, and RHCE and RHD cDNAs were synthesized in two overlapping fragments and sequenced directly as described previously. 1 Sequences were aligned, and protein sequence comparisons were performed with ClustalX or with Sequencher v4.8 (GeneCodes, Ann Arbor, MI).…”

“…Testing of the serum/plasma against RBCs from people whose RHCE alleles have been defined by DNA testing enables further refinement of the specificity of the antibodies. 1-3 …”

RHCE*ceAG (254C>G, Ala85Gly) is prevalent in blacks, encodes a partial ce‐phenotype, and is associated with discordant RHD zygosity

“…Of the 28 patient samples, 17 were referred due to anti‐e or e‐like reactivity in the plasma of patients with e+ RBCs; one had an antibody to a high‐prevalence Rh antigen (P8); one had anti‐C with C+ RBCs (P22) due to partial C antigen; eight had anti‐D with D+ RBCs (P11, P13, P17, P19, P20, P21, P24, P25), and one had variable D RBC typing (P23) due to the presence of the Crawford antigen (RH43) . Two donor samples were referred for investigation of weak e antigen expression (D1, D2), and two donors had C+ RBCs but were negative for the Intron 2 marker diagnostic for RH*C when typed by DNA methods (D3, D4; Table ) that included BeadChip and multiplex methods.…”

Rh and RHAG Blood Group Systems

“…The ceCF complex expresses partial c and e, the low frequency antigen Crawford (RH43), but not its antithetical high frequency antigen CELO (RH58), and some epitopes of D [253,254] . The ceCF complex expresses partial c and e, the low frequency antigen Crawford (RH43), but not its antithetical high frequency antigen CELO (RH58), and some epitopes of D [253,254] .…”