<i>Rhizobium leguminosarum</i>
            Biovar viciae Symbiotic Hydrogenase Activity and Processing Are Limited by the Level of Nickel in Agricultural Soils

Ureta, Ana-Claudia; Imperial, Juan; Ruiz-Argüeso, Tomás; Palacios, José Luis Calvo

doi:10.1128/aem.71.11.7603-7606.2005

Cited by 30 publications

(18 citation statements)

References 17 publications

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“…Hydrogenases are divided into three classes according to the composition and the structure of their catalytic site. Hydrogenases belonging to the [NiFe] class are generally involved in H 2 uptake, are oxygen tolerant (Shima and Thauer, 2007), and nickel availability has been shown to control the H 2 uptake activity of knallgas bacteria that synthesize these enzymes (Friedrich et al, 1981;Brito et al, 1994;Ureta et al, 2005). Because nickel is involved in the maturation process of these enzymes, its presence in excess enhances the formation of their active form (Bö ck et al, 2006;Leach and Zamble, 2007).…”

Section: Discussionmentioning

confidence: 99%

Isolation of Streptomyces sp. PCB7, the first microorganism demonstrating high-affinity uptake of tropospheric H2

2008

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Microbial-mediated soil uptake accounts for ∼80% of the global tropospheric dihydrogen (H2) sinks. Studies conducted over the last three decades provide indirect evidences that H2 soil uptake is mediated by free soil hydrogenases or by unknown microorganisms that have a high affinity for H2. The exact nature of these hypothetical free soil enzymes or of H2-consuming microorganisms remains elusive because the activity has never been observed in pure culture. Here, we present the first aerobic microorganism able to consume tropospheric H2 at ambient levels. A dynamic microcosm chamber was developed to enrich a microbial consortium with a high affinity for H2, from which selected bacterial and fungal strains were isolated and tested for H2 uptake. Strain PCB7 had a H2 consumption activity that followed a Michaelis–Menten kinetics, with an apparent Km of 11 p.p.m.v. and a H2 threshold concentration <0.100 p.p.m.v., corresponding to the high-affinity uptake of tropospheric H2 observed in soil. 16S ribosomal RNA gene sequences showed that strain PCB7 is highly related to several Streptomyces species. H2 consumption occurred during the sporulation period of the bacterium. Addition of nickel increased the activity, suggesting that the enzymes involved in H2 consumption belong to the NiFe uptake class of hydrogenases. Because this is the first microorganism showing a high-affinity uptake of tropospheric H2, we anticipate that Streptomyces sp. PCB7 will become a model organism for the understanding of the environmental factors influencing H2 soil uptake.

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Section: Discussionmentioning

confidence: 99%

Isolation of Streptomyces sp. PCB7, the first microorganism demonstrating high-affinity uptake of tropospheric H2

2008

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“…Deficiency of Ni inhibits urease activity leading to accumulation of urea related N-compounds, which results in toxicity and decreased plant growth (Eskew et al 1983;Brown et al 1990;Dechen et al 2007;Witte 2011). Some authors emphasize the importance of a suitable supply of Ni to increase hydrogenase activity in Bacteroides, increasing nodulation and biological nitrogen fixation (BNF) in soybean (Sellstedt and Smith 1990;Klucas et al 1983;Ureta et al 2005). According to McClure and Israel (1979), the fixed nitrogen transport is done by ureides that catalyze the end of the transport of urea molecule, which is further metabolized by the enzyme urease.…”

Section: Introductionmentioning

confidence: 99%

Effects of nickel fertilization on soybean growth in tropical soils

Levy¹,

Mellis

Murrer

et al. 2019

Bragantia

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Although nickel (Ni) is recognized as plant nutrient since the turn of the 21 st century, uncertainty remains about its optimum application rates and forms. This paper focuses on Ni application in soils, relating to its effect as a plant micronutrient. Specifically, the effect of Ni on the activity of urease in soybean plants is examined.The effects of applying four Ni rates in two different soil types were tested. A full factorial 2 × 4 blocked design experiment was established under controlled conditions. Ni rates (0, 0.25, 0.5 and 1.0 mg of Ni·kg -1 of soil) were applied in two soils with contrasting clay contents. The addition of Ni increased the urease activity in soybean plants but was affected by soil textural differences. The highest urease activity was achieved by the application of 1.0 mg·kg -1 Ni in the sandy soil. The absorption of Ni by the plants and its availability was found to be soil texture dependent. The rate of 0.25 mg·kg -1 Ni increased the soybean dry matter production by 25% in the sandy soil. In conclusion, Ni was effective in promoting plant growth and biomass accumulation although depending on soil clay proportion. For soybean, there was no correlation between urease activity and biomass accumulation.The results of this study indicate a clear Ni effect in different type of soils in São Paulo state, serving as a solid initial doses indicator for soybean fertilization programs and future studies on nickel in Soybean.

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“…Studies indicate that the level of hydrogenase activity can be limited in agricultural soils due to low Ni availability (<0.2 mg kg −1 , extracted by DTPA). Low levels of available Ni in the soil affect the hydrogenase activity, which can be remediated by application of 10 mg dm −3 of Ni (Ureta et al, 2005). However, it has been pointed out in the literature that Ni requirement in all plants vary and it depends on plant species and varieties (Seregin and Kozhevnikova, 2006).…”

Section: Introductionmentioning

confidence: 99%

Soybean Seed Treatment with Nickel Improves Biological Nitrogen Fixation and Urease Activity

Lavres

Franco

Câmara

2016

Front. Environ. Sci.

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Nickel (Ni) is an essential micronutrient required for plants' metabolism due to its role as a structural component of urease and hydrogenase, which in turn perform nitrogen (N) metabolism in many legume species. Seed treatment with cobalt, molybdenum and Bradyrhizobium strains has been widely practiced to improve crops. Additionally, seed treatment together with Ni fertilization of soybean might improve the efficiency of biological nitrogen fixation (BNF), boosting grain dry matter yield, and N content. The objective of this study was to evaluate the effect of soybean seed treatment with Ni rates (0, 45, 90, 135, 180, 360, and 540 mg kg −1 ) on BNF, directly by the 15 N natural abundance method ( 15 δ N‰) and by measurement of urease [E.C. 3.5.1.5] activity, as well as indirectly by nitrogenase (N-ase) activity [E.C. 1.18.6.1]. Soybean plants (cultivar BMX Potência RR) were grown in a sandy soil up to the R7 developmental stage (grain maturity), at which point the nutrient content in the leaves, chlorophyll content, urease, and N-ase activities, Ni and N content in the grains, nodulation (at R1-flowering stage), as well as the contribution of biological nitrogen fixation (BNF; 15 δ N ‰) were evaluated. The proportion of N derived from N 2 fixation varied from 77 to 99% using the natural 15 N abundance method and non-nodulating Panicum miliaceum and Phalaris canariensis as references. A Ni rate of 45 mg kg −1 increased BNF by 12% compared to the control. The increased N uptake in the grains was closely correlated with chlorophyll content in the leaves, urease, and N-ase activities, as well as with nodulation. Grain dry matter yield and aerial part dry matter yield increased, respectively, by 84 and 51% in relation to the control plants at 45 mg kg −1 Ni via seed treatment. Despite, Ni concentration was increased with Ni-seed treatment, Ni rates higher than 135 mg kg −1 promoted negative effects on plant growth and yield. In these experimental conditions, seed treatment with low Ni rates caused higher dry matter yield of plants and grains, N content in the grains, and in the aerial part by increasing of BNF.

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Rhizobium leguminosarum Biovar viciae Symbiotic Hydrogenase Activity and Processing Are Limited by the Level of Nickel in Agricultural Soils

Cited by 30 publications

References 17 publications

Isolation of Streptomyces sp. PCB7, the first microorganism demonstrating high-affinity uptake of tropospheric H2

Isolation of Streptomyces sp. PCB7, the first microorganism demonstrating high-affinity uptake of tropospheric H2

Effects of nickel fertilization on soybean growth in tropical soils

Soybean Seed Treatment with Nickel Improves Biological Nitrogen Fixation and Urease Activity

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