<i>RIM101</i>-Dependent and -Independent Pathways Govern pH Responses in <i>Candida albicans</i>

Davis, Dana A.; Wilson, R. Bryce; Mitchell, Aaron P.

doi:10.1128/mcb.20.3.971-978.2000

Cited by 273 publications

(361 citation statements)

References 28 publications

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“…Targeted integration at the correct genomic locus that led to two disrupted alleles of CaMAC1 was confirmed by PCR analysis using primers CaMAC12344, 3DR and CaARG4+83 (Table 2). Strain BWP17mac1DD/HIS1 was constructed by transforming strain BWP17mac1D : : URA3/mac1D : : ARG4 with pGEM-HIS1 that had been linearized by digestion with NruI (Davis et al, 2000b;Wilson et al, 1999). In order to construct strain BWP17mac1DD/HIS1 : : MAC1, a 2362 bp fragment was amplified by PCR using primers CaMAC12899 and CaMAC1+1443 using …”

Section: Methodsmentioning

confidence: 99%

The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1

Marvin

Cashmore

2004

Microbiology

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The ability of Candida albicans to acquire iron from the hostile environment of the host is known to be necessary for virulence and appears to be achieved using a similar system to that described for Saccharomyces cerevisiae. In S. cerevisiae, high-affinity iron uptake is dependent upon the acquisition of copper. The authors have previously identified a C. albicans gene (CaCTR1) that encodes a copper transporter. Deletion of this gene results in a mutant strain that grows predominantly as pseudohyphae and displays aberrant morphology in low-copper conditions. This paper demonstrates that invasive growth by C. albicans is induced by low-copper conditions and that this is augmented in a Cactr1-null strain. It also shows that deletion of CaCTR1 results in defective iron uptake. In S. cerevisiae, genes that facilitate high-affinity copper uptake are controlled by a copper-sensing transactivator, ScMac1p. The authors have now identified a C. albicans gene (CaMAC1) that encodes a copper-sensing transactivator. A Camac1-null mutant displays phenotypes similar to those of a Cactr1-null mutant and has no detectable CaCTR1 transcripts in low-copper conditions. It is proposed that high-affinity copper uptake by C. albicans is necessary for reductive iron uptake and is transcriptionally controlled by CaMac1p in a similar manner to that in S. cerevisiae.

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Section: Methodsmentioning

confidence: 99%

The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1

Marvin

Cashmore

2004

Microbiology

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“…C. albicans grows in the yeast form in acidic conditions and forms hyphae in neutral/alkaline conditions. At neutral/alkaline pH, such as that encountered in the host, the Rim101 transcription factor is activated by proteolysis of its carboxy-terminal tail (Li and Mitchell 1997;Davis et al 2000). Rim8, Rim13, Rim20, and Rim21 are upstream components required for Rim101 activation (Davis et al 2000;Gomez-Raja and Davis 2012).…”

Section: Environmental Regulation Of Hyphal Morphogenesis Sensing Nutmentioning

confidence: 99%

Fungal Morphogenesis

Lin

Alspaugh

Liu

et al. 2014

Cold Spring Harbor Perspectives in Medicine

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Morphogenesis in fungi is often induced by extracellular factors and executed by fungal genetic factors. Cell surface changes and alterations of the microenvironment often accompany morphogenetic changes in fungi. In this review, we will first discuss the general traits of yeast and hyphal morphotypes and how morphogenesis affects development and adaptation by fungi to their native niches, including host niches. Then we will focus on the molecular machinery responsible for the two most fundamental growth forms, yeast and hyphae. Last, we will describe how fungi incorporate exogenous environmental and host signals together with genetic factors to determine their morphotype and how morphogenesis, in turn, shapes the fungal microenvironment. PROPERTIES OF MORPHOGENESIS IN FUNGAL CELLS

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“…The transcription factor of A. nidulans homologous to PacC in Saccharomyces cerevisiae and C. albicans is Rim101p, the product of the RIM101 gene (also designated PRR2 in C. albicans) (Davis et al, 2000;Porta et al, 1999;Ramó n et al, 1999). Genes known to be under RIM101 regulation in C. albicans include PHR1, PHR2 and PRA1 (Ramó n et al, 1999; Sentandreu et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

The Candida albicans pH-regulated KER1 gene encodes a lysine/glutamic-acid-rich plasma-membrane protein that is involved in cell aggregation

et al. 2004

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Immunoscreening of a Candida albicans cDNA library with a polyclonal germ-tube-specific antibody (pAb anti-gt) resulted in the isolation of a gene encoding a lysine/glutamic-acid-rich protein, which was consequently designated KER1. The nucleotide and deduced amino acid sequences of this gene displayed no significant homology with any other known sequence. KER1 encodes a 134 kDa lysine (14·5 %)/glutamic acid (16·7 %) protein (Ker1p) that contains two potential transmembrane segments. KER1 was expressed in a pH-conditional manner, with maximal expression at alkaline pH and lower expression at pH 4·0, and was regulated by RIM101. A Δker1/Δker1 null mutant grew normally but was hyperflocculant under germ-tube-inducing conditions, yet this behaviour was also observed in stationary-phase cells grown under other incubation conditions. Western blotting analysis of different subcellular fractions, using as a probe a monospecific polyclonal antibody raised against a highly antigenic domain of Ker1p (pAb anti-Ker1p), revealed the presence of a 134 kDa band in the purified plasma-membrane fraction from the wild-type strain that was absent in the homologous preparation from Δker1/Δker1 mutant. The pattern of cell-wall protein and mannoprotein species released by digestion with β-glucanases, reactive towards pAbs anti-gt and anti-Ker1p, as well as against concanavalin A, was also different in the Δker1/Δker1 mutant. Mutant strains also displayed an increased cell-surface hydrophobicity and sensitivity to Congo red and Calcofluor white. Overall, these findings indicate that the mutant strain was affected in cell-wall composition and/or structure. The fact that the ker1 mutant had attenuated virulence in systemic mouse infections suggests that this surface protein is also important in host–fungus interactions.

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RIM101-Dependent and -Independent Pathways Govern pH Responses in Candida albicans

Cited by 273 publications

References 28 publications

The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1

The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1

Fungal Morphogenesis

The Candida albicans pH-regulated KER1 gene encodes a lysine/glutamic-acid-rich plasma-membrane protein that is involved in cell aggregation

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