<i>Salmonella</i>: The Critical Enteric Foodborne Pathogen

Talib, Mohd Afendy Abdul; Radu, Son; Kqueen, Cheah Yoke; Ghazali, Farinazleen Mohamad

doi:10.5772/intechopen.103900

Cited by 2 publications

(3 citation statements)

References 55 publications

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“…is one of the earliest foodborne pathogens described by man. Motile, Gram-negative bacilli are facultative anaerobes growing at a temperature of 5-47 • C, with an optimum of about 37 • C [163,164]. The genus consists of two species, i.e., S. bongori and S. enterica, which include serotypes with the greatest epidemiological significance [17,165].…”

Section: Salmonella Sppmentioning

confidence: 99%

Antibiotic Resistance in Selected Emerging Bacterial Foodborne Pathogens—An Issue of Concern?

et al. 2023

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Antibiotic resistance (AR) and multidrug resistance (MDR) have been confirmed for all major foodborne pathogens: Campylobacter spp., Salmonella spp., Escherichia coli and Listeria monocytogenes. Of great concern to scientists and physicians are also reports of antibiotic-resistant emerging food pathogens—microorganisms that have not previously been linked to food contamination or were considered epidemiologically insignificant. Since the properties of foodborne pathogens are not always sufficiently recognized, the consequences of the infections are often not easily predictable, and the control of their activity is difficult. The bacteria most commonly identified as emerging foodborne pathogens include Aliarcobacter spp., Aeromonas spp., Cronobacter spp., Vibrio spp., Clostridioides difficile, Escherichia coli, Mycobacterium paratuberculosis, Salmonella enterica, Streptocccus suis, Campylobacter jejuni, Helicobacter pylori, Listeria monocytogenes and Yersinia enterocolitica. The results of our analysis confirm antibiotic resistance and multidrug resistance among the mentioned species. Among the antibiotics whose effectiveness is steadily declining due to expanding resistance among bacteria isolated from food are β-lactams, sulfonamides, tetracyclines and fluoroquinolones. Continuous and thorough monitoring of strains isolated from food is necessary to characterize the existing mechanisms of resistance. In our opinion, this review shows the scale of the problem of microbes related to health, which should not be underestimated.

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Section: Salmonella Sppmentioning

confidence: 99%

Antibiotic Resistance in Selected Emerging Bacterial Foodborne Pathogens—An Issue of Concern?

et al. 2023

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“…24 Specifically, in B. subtilis, the integration of the CRISPR/dCas9 system with CRISPR-assisted oligonucleotide annealing-based promoter shuffling (OAPS) for pathway optimization has led to a substantial enhancement in the to conditions from mild gastroenteritis to severe, lifethreatening systemic infections. 27 The infection of human intestinal epithelial Caco-2 cells by Salmonella, a model system that closely simulates in vivo conditions, offers crucial insights into host−pathogen interactions. 28 Salmonella expertly manipulates host cellular mechanisms to invade, survive, and proliferate within the host cells.…”

Section: Introductionmentioning

confidence: 99%

“…Salmonella , a genus of rod-shaped bacteria, is a well-known cause of global foodborne illnesses. These pathogens can lead to conditions from mild gastroenteritis to severe, life-threatening systemic infections . The infection of human intestinal epithelial Caco-2 cells by Salmonella , a model system that closely simulates in vivo conditions, offers crucial insights into host–pathogen interactions …”

Section: Introductionmentioning

confidence: 99%

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

Liao,

Cui,

Gao

et al. 2024

J. Agric. Food Chem.

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This study optimized the menaquinone-7 (MK-7) synthetic pathways in Bacillus subtilis (B. subtilis) natto NB205, a strain that originated from natto, to enhance its MK-7 production. Utilizing mutation breeding, we developed NBMK308, a mutant strain that demonstrated a significant 117.23% increase in MK-7 production. A comprehensive transcriptome analysis identified two key genes, ispA and ispE, as being critical in MK-7 synthesis. The dual-sgRNA CRISPRa system was utilized to achieve precise regulation of ispA and ispE in the newly engineered strain, A3E3. This strategic modulation resulted in a significant enhancement of MK-7 production, achieving increases of 20.02% and 201.41% compared to traditional overexpression systems and the original strain NB205, respectively. Furthermore, the fermentation supernatant from A3E3 notably inhibited Salmonella invasion in Caco-2 cells, showcasing its potential for combating such infections. The safety of the dual-sgRNA CRISPRa system was confirmed through cell assays. The utilization of the dual-sgRNA CRISPRa system in this study was crucial for the precise regulation of key genes in MK-7 synthesis, leading to a remarkable increase in production and demonstrating additional therapeutic potential in inhibiting pathogenic infections. This approach effectively combined the advantages of microbial fermentation and biotechnology, addressing health and nutritional challenges.

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Salmonella: The Critical Enteric Foodborne Pathogen

Cited by 2 publications

References 55 publications

Antibiotic Resistance in Selected Emerging Bacterial Foodborne Pathogens—An Issue of Concern?

Antibiotic Resistance in Selected Emerging Bacterial Foodborne Pathogens—An Issue of Concern?

Dual-sgRNA CRISPRa System for Enhanced MK-7 Production and Salmonella Infection Mitigation in Bacillus subtilis natto Applied to Caco-2 Cells

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