<i>Schistosoma mansoni</i>: host antigen occurrence on worms recovered from laboratory vertebrate animals

Coelho, Paulo Marcos Zech; Gazzinelli, Giovanni; Pellegrino, J.

doi:10.1017/s0031182000056080

Cited by 8 publications

(6 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This ex tent of reactivity by this antiserum may result from the fact that the adult worm (used for immunization) can acquire blood group antigens [38] and other host antigens [40,41].…”

Section: Discussionmentioning

confidence: 99%

Parasitic Modulation of Host Immune Mechanisms in Schistosomiasis

Attallah¹,

Abdul-Aal²,

Urritia-Shaw³

et al. 1987

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Antigen antibody complexes are suspected to play a role in the pathogenesis of some of the lesions that result from schistosomiasis. To examine the effect of immune complexes on the immune system of mice experimentally infected with Schistosoma mansoni, we measured antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-mediated serum hemolysis in normal and infected animals. ADCC activity in infected mice was depressed compared to control mice. However, preincubation of spleen cells for 24 h in medium followed by washing restored ADCC activity. This suggested that a soluble factor(s), presumably immune complexes, was bound to the Fc receptors with resultant block in ADCC activity and this was removed in vitro during the 24-hour preincubation. Furthermore, the complement activity of mouse serum was markedly depressed in mice infected for 3 or 6 weeks. Again, the presence of immune complexes could explain this depression since immune complexes bind complement. We attempted to confirm and extend these findings with an immunoperoxidase-staining technique using antibody to S. mansoni antigen. Most of the granuloma formations identified in portal tracts and intestinal mucosa were composed of macrophages and epithelial cells surrounding a central nidus of schistosome egg. In addition, schistosome antigen was seen diffusely bound to some of the lymphoid elements in the lamina propria and many of these cells appeared plasmacytoid. Furthermore, large amounts of schistosome antigen were sequestered in the medullary cords of the mesenteric lymph nodes and in the Billroth cords of the spleen. This suggests that the antigen is conveyed to the lymph nodes and the spleen through the systemic circulation. Taken together, these findings suggest the circulating immune complexes are a feature of schistosomiasis and may well contribute to the associated immunopathology.

show abstract

“…This ex tent of reactivity by this antiserum may result from the fact that the adult worm (used for immunization) can acquire blood group antigens [38] and other host antigens [40,41].…”

Section: Discussionmentioning

confidence: 99%

Parasitic Modulation of Host Immune Mechanisms in Schistosomiasis

Attallah¹,

Abdul-Aal²,

Urritia-Shaw³

et al. 1987

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…Electron microscopy using ferritin as an immune marker confirmed that mouse antigens were indeed present on the surfaces of the mouse-derived schistosomes prior to transfer (PL 1), and further demonstrated that the immune attack mounted against the parasites by the anti-mouse monkeys was surface-directed (PL 2). The existence of host determinants on the schistosome surface has since been amply confirmed in a variety of other donor/recipient models (Cioli & Neis, 1972;Erickson, Beattie, Yamaguchi, Miyasaka & Williams, 1973;Boyer, Kalfayan & Ketchum, 1977;Lewert, Yogore, Para & Ozeel, 1977;Coelho et al 1980). Subsequent studies, both in vivo and in vitro, demonstrated that the host molecules acquired by the schistosomes were in fact surface components of the erythrocyte; A, B, H and Lewis b+ antigens were acquired by the worms, whereas the Rhesus, M, N, S and Duffy antigens were not (Clegg, Smithers & Terry, 1971;Goldring, Clegg, Smithers & Terry, 1976).…”

Section: Acquired Host Determinantsmentioning

confidence: 94%

“…Although Damian's hypothesis received criticism on the grounds that Schistosoma mansoni is primarily a parasite of humans rather than rodents (Williams & Soulsby, 1970;Read, 1972;Clegg, 1974;Smithers & Terry, 1976), Damian defended his views by proposing that antigen sharing between 8. mansoni and the mouse is a reflexion of a prior evolutionary history of association between these parasites and rodents and that the prevalence of schistosomes in man is a more recent development (Damian, 1979). The arguments put forward by Damian to support this proposal, however, tend not to be supported by contemporary data (Coelho, Gazzinelli & Pellegrino, 1980). Experiments in vitro involving labelled amino acid precursors demonstrated that hamster-derived schistosomes could utilize such precursors to synthesize a protein that cross-reacted with hamster liver protein (Bout, Capron, Dupas & Capron, 1974).…”

Section: Synthesized Shared Determinantsmentioning

confidence: 95%

Disguise as an evasive stratagem of parasitic organisms

McLaren

1984

Parasitology

View full text Add to dashboard Cite

It is now well established that animal parasites elicit vigorous immune responses in their hosts and that the resistance to reinfection thus acquired is mediated through conventional humoral and/or cellular effector mechanisms. It is also recognized, however, that some parasites exhibit extreme longevity in the face of a potentially hostile or lethal environment, and it may be assumed therefore that such organisms possess a repertoire of highly sophisticated and successful evasive strategies. Of all the survival mechanisms thus far proposed, the concept of antigen sharing, or disguise, is probably the most contentious. Arguments have arisen over the nature and origin of the shared determinants and over the validity of their role in ensuring parasite survival within the fully immunocompetent host. This presentation reviews published data concerning the existence of a disguise stratagem amongst medically important parasites and evaluates experiments designed to investigate the functional importance of disguise in immune evasion.

show abstract

“…They suggested that these host antigens are truly of host origin and are acquired by the worms during their development in the host, and that these species-specific host antigens are firmly bound or even incorporated at the tegumental surface of schistosomula and adults of S. mansoni. This close association of host antigens and schistosome surfaces has been confirmed by many investigators, demonstrating the presence of mouse immunoglobulins on the surface of adult worms (Kemp, Damian & Greene, 1976a;Songadares-Bernal, 1976;Kemp et al 1977Kemp et al , 1980Kemp, Merritt & Rosier, 1978), blood group antigens on the surface of schistosomula and adults (Clegg, Smithers & Terry, 1970, 1971Dean, 1974;McLaren, Clegg & Smithers, 1975;Goldring et al 1976;Coelho, Gazzinelli & Pellegrino, 1980), Forssman-like antigen , intracellular substance antigen (Smith & Kusel, 1979) and the gene products of the mouse major histocompatibility complex (Sher, Hall & Vadas, 1978;Gitter & Damian, 1982;Gitter, McCormick & Damian, 1982;Simpson et al 1983). The presence of Fc receptors was also demonstrated on the surface of adults (Kemp et al 1977) and schistosomula (Torpier, Capron & Ouaissi, 1979) of £.…”

Section: Discussionmentioning

confidence: 61%

Host-related DNA sequences are localized in the body of schistosome adults

Irie

Iwamura

1993

Parasitology

View full text Add to dashboard Cite

The localization of host (mouse)-related DNA sequences in the adults and cercariae of Schistosoma japonicum and S. mansoni was examined by in situ hybridization using 32P-labelled probes. The hybridization signals to the sequences of the mouse type C and type A retroviruses were clearly observed in the subtegumental layer and inner tissues of S. japonicum adults. In contrast, it was hard to find any signals to these sequences in S. mansoni adults. Distinct signals to the env-specific region of the mouse ecotropic type C retrovirus were observed in the subtegumental layer and inner tissues of S. mansoni adults. No signal to the sequence of the mouse type B retrovirus was found on the sections of either schistosome adult. The signals to the sequence of the genomic clone SmM51 derived from male S. mansoni were found in the tissues of both the schistosome adults. The signals to the sequences of the mouse type A and env-specific region of ecotropic type C retroviruses were not seen in the cercariae of each species, whereas the signals to the sequence of SmM51 were detected in S. mansoni cercariae. These observations suggest that host-related DNA sequences might be incorporated unequally into the schistosome genome during development in the final host.

show abstract

Schistosoma mansoni: host antigen occurrence on worms recovered from laboratory vertebrate animals

Cited by 8 publications

References 8 publications

Parasitic Modulation of Host Immune Mechanisms in Schistosomiasis

Parasitic Modulation of Host Immune Mechanisms in Schistosomiasis

Disguise as an evasive stratagem of parasitic organisms

Host-related DNA sequences are localized in the body of schistosome adults

Contact Info

Product

Resources

About