<i><scp>NKG</scp>2C</i> gene deletion in the Mexican population and lack of association to respiratory viral infections

Rangel-Ramírez, Verónica; García-Sepúlveda, Christian A.; Escalante-Padrón, Francisco J.; Pérez-González, Luis Fernando; Rangel-Castilla, A.; Aranda‐Romo, Saray; Noyola, Daniel E.

doi:10.1111/iji.12104

Cited by 17 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results confirm findings from a recent report in which the prevalence of the NKG2C gene deletion in Mexican individuals was approximately 50% lower than in other populations 13 . A slight difference in the proportion of subjects with two copies between students with and without CMV infection was observed in the present study; however, this was not statistically significant.…”

Section: Analysis Of Risk Factors Associated To Cytomegalovirus Infecsupporting

confidence: 92%

See 1 more Smart Citation

Analysis of risk factors associated to cytomegalovirus infection in dentistry students.

Tejera-Hernández¹,

Noyola²,

Sánchez-Vargas³

et al. 2015

J Oral Res

View full text Add to dashboard Cite

Section: Analysis Of Risk Factors Associated To Cytomegalovirus Infecsupporting

confidence: 92%

“…This assay uses two sets of primers: a set that amplifies a 411-bp fragment when the NKG2C gene is absent and another set of primers that amplifies a 201-bp fragment of the NKG2C gene. The PCR conditions used were those described by Rangel-Ramírez et al 13 .…”

Section: Methodsmentioning

confidence: 99%

Analysis of risk factors associated to cytomegalovirus infection in dentistry students.

Tejera-Hernández¹,

Noyola²,

Sánchez-Vargas³

et al. 2015

J Oral Res

View full text Add to dashboard Cite

“…We did not find any differences in the expression of NKG2C, a marker of activation associated with viral infections, between HEU and HUU infants. However, deletion of the NKG2C gene has been reported with varying prevalence in different populations (42); this may have limited our ability to detect differences in the expression of this marker.…”

Section: Discussionmentioning

confidence: 99%

Altered Natural Killer Cell Function in HIV-Exposed Uninfected Infants

et al. 2017

View full text Add to dashboard Cite

ObjectivesHIV-exposed uninfected (HEU) infants have higher rates of severe and fatal infections compared with HIV-unexposed (HUU) infants, likely due to immune perturbations. We hypothesized that alterations in natural killer (NK) cell activity might occur in HEU infants and predispose them to severe infections.DesignCase–control study using cryopreserved peripheral blood mononuclear cells (PBMCs) at birth and 6 months from HEU infants enrolled from 2002 to 2009 and HUU infants enrolled from 2011 to 2013.MethodsNK cell phenotype and function were assessed by flow cytometry after 20-h incubation with and without K562 cells.ResultsThe proportion of NK cells among PBMCs was lower at birth in 12 HEU vs. 22 HUU (1.68 vs. 10.30%, p < 0.0001) and at 6 months in 52 HEU vs. 72 HUU (3.09 vs. 4.65%, p = 0.0005). At birth, HEU NK cells demonstrated increased killing of K562 target cells (p < 0.0001) and increased expression of CD107a (21.65 vs. 12.70%, p = 0.047), but these differences resolved by 6 months. Stimulated HEU NK cells produced less interferon (IFN)γ at birth (0.77 vs. 2.64%, p = 0.008) and at 6 months (4.12 vs. 8.39%, p = 0.001), and showed reduced perforin staining at 6 months (66.95 vs. 77.30%, p = 0.0008). Analysis of cell culture supernatants indicated that lower NK cell activity in HEU was associated with reduced interleukin (IL)-12, IL-15, and IL-18. Addition of recombinant human IL-12 to stimulated HEU PBMCs restored IFNγ production to that seen in stimulated HUU cultures.ConclusionNK cell proportion, phenotype, and function are altered in HEU infants. NK cell cytotoxicity and degranulation are increased in HEU at birth, but HEU NK cells have reduced IFNγ and perforin production, suggesting an adequate initial response, but decreased functional reserve. NK cell function improved with addition of exogenous IL-12, implicating impaired production of IL-12 by accessory cells. Alterations in NK cell and accessory cell function may contribute to the increased susceptibility to infection in HEU infants.

show abstract

“…The 29.3% haplotype frequency of the NKG2C deletion in our African cohort is higher than that recorded elsewhere. 33,35,55 Whether the frequency of this haplotype is linked to current or historic intensities of HCMV infection might also merit further investigation.…”

mentioning

confidence: 99%

Rapid NK cell differentiation in a population with near-universal human cytomegalovirus infection is attenuated by NKG2C deletions

Goodier

White

Darboe

et al. 2014

Blood

103

View full text Add to dashboard Cite

Key Points• HCMV infection in early life is associated with rapid phenotypic and functional differentiation of NK cells. • Emergence of CD571 NK cells is attenuated in children lacking NKG2C.Natural killer (NK) cells differentiate and mature during the human life course; human cytomegalovirus (HCMV) infection is a known driver of this process. We have explored human NK cell phenotypic and functional maturation in a rural African (Gambian) population with a high prevalence of HCMV. The effect of age on the frequency, absolute number, phenotype, and functional capacity of NK cells was monitored in 191 individuals aged from 1 to 49 years. Increasing frequencies of NK cells with age were associated with increased proportions of CD56 dim cells expressing the differentiation marker CD57and expansion of the NKG2C 1 subset. Frequencies of NK cells responding to exogenous cytokines declined with age in line with a decreased proportion of CD57 2 cells. These changes coincided with a highly significant drop in anti-HCMV IgG titers by the age of 10 years, suggesting that HCMV infection is brought under control as NK cells differentiate (or vice versa). Deletion at the NKG2C locus was associated with a gene dose-dependent reduction in proportions of CD94 1 and CD57 1 NK cells. Importantly, anti-HCMV IgG titers were significantly elevated in NKG2C 2/2 children, suggesting that lack of expression of NKG2C may be associated with altered control

show abstract

NKG2C gene deletion in the Mexican population and lack of association to respiratory viral infections

Cited by 17 publications

References 22 publications

Analysis of risk factors associated to cytomegalovirus infection in dentistry students.

Analysis of risk factors associated to cytomegalovirus infection in dentistry students.

Altered Natural Killer Cell Function in HIV-Exposed Uninfected Infants

Rapid NK cell differentiation in a population with near-universal human cytomegalovirus infection is attenuated by NKG2C deletions

Contact Info

Product

Resources

About