Short Communication:Maintenance of Glycoprotein-Determined Phenotype in an HIV Type 1 (pNL43)envGene-Cassetting System

Abstract

Here we report the construction and use of a full-length env gene-cassetting system, C2, based on the HIV-1 infectious molecular clone NL43. C2 produces virus with the same phenotype as NL43 but with 2-fold lower growth kinetics. The latter probably relates to alteration in the vpu and/or nef genes. C2-env chimeras of macrophage-tropic and T cell-tropic laboratory strains and primary HIV-1 isolates retain the glycoprotein-determined phenotypes of their parent viruses. The cassette will assist studies of HIV-1 pathogenesis.

Structural features and anti-HIV-1 activity of novel polysaccharides from red algae Grateloupia longifolia and Grateloupia filicina

“…HIV-1 PE106 (PE106, subtype B, X4, PI) that had undergone a single passage in PBMCs in which it induces syncytia [16], was used to evaluate the antiviral activity of the sulphated polysaccharides. Virus stocks were prepared in CD8-depleted PBMC previously stimulated with PHA (5 g/ml, Sigma) for 3 days as described [17].…”

Neutralization activity in a geographically diverse East London cohort of human immunodeficiency virus type 1-infected patients: clade C infection results in a stronger and broader humoral immune response than clade B infection

“…Construction of patient gp160-derived infectious chimaeric molecular clones. K530(6) (Zimbabwe, clade C, CCR5) and L120 (Congo, CRF02_AG, CCR5) are molecular clones where the gp160 genes were amplified from genomic DNA from patient blood (infected for .1 year) using nested PCR, as described previously (Zheng & Daniels, 2001), with some modifications (inner forward 59-AGC-TCGAGCAGAAGACAGTGGCAATGA-39 and inner reverse 59-CTA-TGGAATTCTTCGACCACTTGCCCCCATTT-39). The PCR products were subcloned into TopoTA vector (Invitrogen) and transferred into the C2/NL43 vector (Zheng & Daniels, 2001) between the XhoI and EcoRI sites.…”

An HIV Type 1 Subtype B Founder Effect in Korea: gp160 Signature Patterns Infer Circulation of CTL-Escape Strains at the Population Level

“…19 The coreceptor usage of the progeny viruses was assessed in Ghost cells expressing human CD4 and one of a range of chemokine coreceptors. 20…”