<i>spn-F</i>encodes a novel protein that affects oocyte patterning and bristle morphology in<i>Drosophila</i>

Bar, Dikla; Schüpbach, Trudi

doi:10.1242/dev.02319

Cited by 35 publications

(91 citation statements)

References 43 publications

(52 reference statements)

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“…Similarly, mutants with defective microtubule organization have been shown to affect the translation of grk mRNA. In these mutants, grk mRNA is inefficiently translated (Abdu et al 2006;Klattenhoff et al 2007;Pane et al 2007). It is therefore possible that the aberrant microtubule organization present in these Egl-depleted egg chambers underlies the defective translational regulation of osk and grk mRNAs.…”

Section: Egl and Translation Regulationmentioning

confidence: 99%

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

et al. 2016

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The Drosophila egg chamber provides a useful model for examining mechanisms by which cell fates are specified and maintained in the context of a complex tissue. The egg chamber is also an excellent model for understanding the mechanism by which cytoskeletal filaments are organized and the critical interplay between cytoskeletal organization, polarity establishment, and cell fate specification. Previous work has shown that Egalitarian (Egl) is required for specification and maintenance of oocyte fate. Mutants in egl either completely fail to specify an oocyte, or if specified, the oocyte eventually reverts back to nurse cell fate. Due to this very early role for Egl in egg chamber maturation, it is unclear whether later stages of egg chamber development also require Egl function. In this report, we have depleted Egl at specific stages of egg chamber development. We demonstrate that in early-stage egg chambers, Egl has an additional role in organization of oocyte microtubules. In the absence of Egl function, oocyte microtubules completely fail to reorganize. As such, the localization of microtubule motors and their cargo is disrupted. In addition, Egl also appears to function in regulating the translation of critical polarity determining messenger RNAs (mRNAs). Finally, we demonstrate that in midstage egg chambers, Egl does not appear to be required for microtubule organization, but rather for the correct spatial localization of oskar, bicoid, and gurken mRNAs.

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Section: Egl and Translation Regulationmentioning

confidence: 99%

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

et al. 2016

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“…RNA in situ hybridization on ovaries and antibody staining of cells and ovaries was carried out as described previously (Abdu et al, 2006;DubinBar et., 2008). For bristle immunostaining, tissues were treated as described by Bitan et al (Bitan et al, 2010).…”

Section: Immunostaining and In Situ Hybridizationmentioning

confidence: 99%

“…For bristle immunostaining, tissues were treated as described by Bitan et al (Bitan et al, 2010). The following primary antibodies were used: rabbit anti-Jvl (1:100), mouse anti-Spn-F (1:10, clone 8C10) (Abdu et al, 2006), mouse anti-Grk (1:10, clone 1D12) (Queenan et al, 1999), rabbit anti-Oskar (1:3000) (Vanzo and Ephrussi, 2002), mouse anti--tubulin (1:200, Sigma), mouse anti -Gal (1:500, Promega), mouse antiOrb 4H8 and 6H4 (1:10) (Lantz et al, 1992), rat anti HA (1:200, Roche Diagnostics) and rabbit anti Jvl (1:100). The secondary antibodies goat anti-mouse Cy2 and Cy3, and goat anti-rabbit Cy3 (Jackson ImmunoResearch) were used at a dilution of 1:100.…”

Section: Immunostaining and In Situ Hybridizationmentioning

confidence: 99%

“…Previously, we and others have shown that mutations in spindle-F (spn-F) and in the Drosophila IKK homologue ik2, affect both egg chamber polarity and bristle development (Abdu et al, 2006;Shapiro and Anderson, 2006). It has been demonstrated that the defects in egg chamber development in spn-F and ik2 are attributed to their effect on mRNA localization by regulating oocyte cytoskeleton organization.…”

Section: Introductionmentioning

confidence: 99%

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Drosophila javelin-like encodes a novel microtubule-associated protein and is required for mRNA localization during oogenesis

et al. 2011

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SUMMARYAsymmetrical localization of mRNA transcripts during Drosophila oogenesis determines the anteroposterior and dorsoventral axes of the Drosophila embryo. Correct localization of these mRNAs requires both microtubule (MT) and actin networks. In this study, we have identified a novel gene, CG43162, that regulates mRNA localization during oogenesis and also affects bristle development. We also showed that the Drosophila gene javelin-like, which was identified based on its bristle phenotype, is an allele of the CG43162 gene. We demonstrated that female mutants for jvl produce ventralized eggs owing to the defects in the localization and translation of gurken mRNA during mid-oogenesis. Mutations in jvl also affect oskar and bicoid mRNA localization. Analysis of cytoskeleton organization in the mutants reveal defects in both MT and actin networks. We showed that Jvl protein colocalizes with MT network in Schneider cells, in mammalian cells and in the Drosophila oocyte. Both in the oocyte and in the bristle cells, the protein localizes to a region where MT minus-ends are enriched. Jvl physically interacts with SpnF and is required for its localization. We found that overexpression of Jvl in the germline affects MT-dependent processes: oocyte growth and oocyte nucleus anchoring. Thus, our results show that we have identified a novel MT-associated protein that affects mRNA localization in the oocyte by regulating MT organization.

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“…Previous genetic screens based on female sterility and the spindle phenotype also identified DV polarity genes with functions other than DSB repair Wieschaus 1989, 1991;Morris et al 2003;Staeva-Vieira et al 2003). These included genes required for grk mRNA transport (Swan and Suter 1996;Swan et al 1999;Brendza et al 2000;1 Huynh and St Johnston 2000; Navarro et al 2004;Abdu et al 2006), genes required for Grk processing (Styhler et al 1998;Miura et al 2006), and genes regulating the stability and trafficking of Grk protein (Saunders and Cohen 1999;Kennerdell et al 2002;Findley et al 2003;Wilhelm et al 2005;Bokel et al 2006).…”

Section: A S Cells Divide Checkpoints Delay the Transition Tomentioning

confidence: 99%

A Maternal Screen for Genes Regulating Drosophila Oocyte Polarity Uncovers New Steps in Meiotic Progression

2007

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Meiotic checkpoints monitor chromosome status to ensure correct homologous recombination, genomic integrity, and chromosome segregation. In Drosophila, the persistent presence of double-strand DNA breaks (DSB) activates the ATR/Mei-41 checkpoint, delays progression through meiosis, and causes defects in DNA condensation of the oocyte nucleus, the karyosome. Checkpoint activation has also been linked to decreased levels of the TGFa-like molecule Gurken, which controls normal eggshell patterning. We used this easy-toscore eggshell phenotype in a germ-line mosaic screen in Drosophila to identify new genes affecting meiotic progression, DNA condensation, and Gurken signaling. One hundred eighteen new ventralizing mutants on the second chromosome fell into 17 complementation groups. Here we describe the analysis of 8 complementation groups, including Kinesin heavy chain, the SR protein kinase cuaba, the cohesin-related gene dPds5/cohiba, and the Tudor-domain gene montecristo. Our findings challenge the hypothesis that checkpoint activation upon persistent DSBs is exclusively mediated by ATR/Mei-41 kinase and instead reveal a more complex network of interactions that link DSB formation, checkpoint activation, meiotic delay, DNA condensation, and Gurken protein synthesis.

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spn-Fencodes a novel protein that affects oocyte patterning and bristle morphology inDrosophila

Cited by 35 publications

References 43 publications

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber

Drosophila javelin-like encodes a novel microtubule-associated protein and is required for mRNA localization during oogenesis

A Maternal Screen for Genes Regulating Drosophila Oocyte Polarity Uncovers New Steps in Meiotic Progression

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