<i>Staphylococcus aureus</i> ST6-t701 Isolates from Food-Poisoning Outbreaks (2006–2013) in Xi'an, China

Li, Guanghui; Shou-zhi, WU; Wen, Luo; Su, Yulan; Luan, Yang; Wang, Xin

doi:10.1089/fpd.2014.1850

Cited by 36 publications

(29 citation statements)

References 10 publications

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“…The hybridization pattern of the isolate was identical to the predicted hybridization pattern for clinical CC6-MSSA isolates 394_SAUR (GenBank JVIV) and C9 (GenBank LDVH). It harbored the beta-lactamase operon as well as the IEC genes sea , sak and scn .CC6 has been recovered occasionally from humans including butchers [117] and it has been implicated in episodes of food-poisoning [118]. It was previously identified in camels from Dubai, where it carried a different sea allele [21], as well as in non-human primates and various domestic animals [4,119].…”

Section: Resultsmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST). The isolates were assigned to 29 clonal complexes and singleton sequence types (CC1, CC5, CC6, CC7, CC8, CC9, CC12, CC15, CC22, CC25, CC30, CC49, CC59, CC88, CC97, CC130, CC133, CC398, ST425, CC599, CC692, CC707, ST890, CC1956, ST2425, CC2671, ST2691, CC2767 and ST2963), some of which (ST2425, ST2691, ST2963) were not described previously. Resistance rates in wildlife strains were rather low and mecA-MRSA isolates were rare (n = 6). mecC-MRSA (n = 8) were identified from a fox, a fallow deer, hares and hedgehogs. The common cattle-associated lineages CC479 and CC705 were not detected in wildlife in the present study while, in contrast, a third common cattle lineage, CC97, was found to be common among cervids. No Staphylococcus argenteus or Staphylococcus schweitzeri-like isolates were found. Systematic studies are required to monitor the possible transmission of human- and livestock-associated S. aureus/MRSA to wildlife and vice versa as well as the possible transmission, by unprotected contact to animals. The prevalence of S. aureus/MRSA in wildlife as well as its population structures in different wildlife host species warrants further investigation.

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Section: Resultsmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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“…The involvement of see in food poisoning has rarely been reported in China. However, three cases of food poisoning caused by SEE were reported in USA, France (Bergdoll et al, 1971; Morris et al, 1972) and Shaanxi, China (Li et al, 2015). Similarly, Ostyn et al (2010) reported that 90 ng of SEE can lead to food poisoning.…”

Section: Discussionmentioning

confidence: 99%

“…Fibronectin-binding proteins have been shown to have important roles in the establishment of infections and persistent infections; α -hemolysin leads to disruption of the epithelial barrier and homeostasis, which in turn lead to necrotic cell death and systemic infection (Kong et al, 2016); and PVL is a pore-forming cytotoxin. Notably, the hlα gene was reported to be responsible for an S. aureus food poisoning outbreak in Xi’an, China (Li et al, 2015). Indeed, approximately 90.0% of S. aureus induced necrotizing pneumonia is associated with isolation of S. aureus strains carrying the pvl gene (Bhatta et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Prevalence, Virulence Genes, Antimicrobial Susceptibility, and Genetic Diversity of Staphylococcus aureus from Retail Aquatic Products in China

Rong

et al. 2017

Front. Microbiol.

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Staphylococcus aureus is an important food-borne opportunistic pathogen that frequently causes severe blood and tissue infections or even fatal illnesses. Although S. aureus has been extensively studied in livestock and poultry foods in China, limited information has been reported in aquatic products. Accordingly, in this study, we aimed to characterize S. aureus in aquatic products purchased from retail markets in China. In total, 320 aquatic food samples were collected from 32 provincial capitals in China. The results showed that 119 samples (37.2%, 119/320) were positive for S. aureus by both qualitative and quantitative analyses. The contamination levels of 78.2% of samples ranged from 0.3 to 10 MPN/g, and six samples exceeded 110 MPN/g. A total of 119 S. aureus isolates from positive samples were selected to evaluate virulence factors, antibiotic resistance, and molecular characteristics. All S. aureus isolates were evaluated for the presence of 11 virulence genes by multiplex polymerase chain reaction, and α-hemolysin (hlα, 84.9%), fibronectin-binding protein A (fnbA, 79.0%), S. aureus enterotoxin E (see, 53.8%), and Panton-Valentine leucocidin (pvl, 50.4%) were identified as the major genes. These genes formed 56 different profiles, with the major profile identified as pvl-hlα-fnbA (28.6%). The antimicrobial susceptibility of all isolates was analyzed through the disk diffusion method, and the results showed high resistance to β-lactams, macrolides and tetracyclines, but susceptibility to linezolid and vancomycin. In addition, 26 sequence types (STs) were obtained via multilocus sequence typing, including seven novel STs, among which ST1 (20.2%), ST15 (18.5%), and ST188 (13.4%) were the most common STs. All the isolates were mecC negative, but nine isolates carrying mecA were evaluated by staphylococcal cassette chromosome mec (SCCmec) typing, all of which were SCCmecIII or SCCmecIV types. Isolates of SCCmecIII showed a high prevalence and were multidrug resistant. Our results showed that aquatic products could be a vehicle for transmission of virulence genes and multidrug-resistant S. aureus, representing a potential public health risk. The STs identified in this study indicated the genetic diversity of S. aureus, thereby providing important basic data for the dissemination of S. aureus in aquatic products.

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“…Out of 60 isolates, 9 (15%) were positive for the PCR amplicon of 310 bp for hlb gene and 7 (11.67%) were positive for the amplicon of 938 bp for hlg (Figure 3). Li et al (2015) reported that food poisoning outbreaks in China were caused by hla and hld genes.…”

Section: Resultsmentioning

confidence: 99%

In Silico Virulence and Resistance Profile Analysis of Staphylococcus species

Nahar¹,

Rashid²,

Kabir³

et al. 2017

Bangla Pharma J

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In silico studies of the genes of Staphylococcus spp. might establish some correlations with multiple pathological factors. Sixty isolates of Staphylococcus spp. have been studied here targeting virulence and antibiotic resistance genes through in silico tools. Here, in silico PCR (polymerase chain reaction) amplification detected both virulence and antibiotic resistance genes. Study revealed that most of the isolates harboured either cap5 (40%) or cap8 (31.67%) locus gene. Staphylococcal enterotoxin was detected in 63.33% of the isolates. The sea gene, responsible for food poisoning, was detected in 26.67% of the isolates. The tst positive isolates (5%), responsible for toxic shock syndrome, were present in only genotype 8. No exfoliative toxin was detected. The icaA gene, responsible for intracellular adherence, appeared in 80% of the isolates. Alpha hemolysin gene, hla, was detected in 63.33% of the isolates. Sixty-five percent of the isolates harboured the mecA genes. Both β-lactamase (blaZ) and erythromycin resistance, ermA genes were available in 38.33% of the isolates. In silico pulsed field gel electrophoresis (PFGE) digestion was able to divide isolates into 23 genotypes. Genotype 8 and 11 harboured tetracycline resistance genes, tetM and tetK. The tetM gene (18.33%) was more prevalent than tetK gene (11.67%). Genotype 1 and 11 were considered more virulent than others. Genotype 11 also carried six antibiotic resistance genes but did not carry the genes msrA, msrB, ermB and ermC. The data generated here might aid in the prediction of the virulence and resistance profile based on genotyping as well as contribute in vaccine development.

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Staphylococcus aureus ST6-t701 Isolates from Food-Poisoning Outbreaks (2006–2013) in Xi'an, China

Cited by 36 publications

References 10 publications

Diversity of Staphylococcus aureus Isolates in European Wildlife

Diversity of Staphylococcus aureus Isolates in European Wildlife

Prevalence, Virulence Genes, Antimicrobial Susceptibility, and Genetic Diversity of Staphylococcus aureus from Retail Aquatic Products in China

In Silico Virulence and Resistance Profile Analysis of Staphylococcus species

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