The aim of the study was to investigate the molecular epidemiologic of Staphylococcus aureus isolated from seven staphylococcal food-poisoning (SFP) outbreaks between 2006 and 2013 in Xi'an, northwest China. A total of seven S. aureus isolates associated with seven SFP outbreaks were obtained and characterized by determining the multilocus sequence typing, spa typing, pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility, toxin and resistant genes. The results showed that S. aureus ST6-t701 (71.4%) was the most predominant clone, followed by ST5-t002 and ST59-t172 (each 14.3%). Seven different PFGE patterns were obtained. Moreover, resistance was most frequently observed to trimethoprim and penicillin (each 71.4%), followed by erythromycin (28.6%), ampicillin, clindamycin, and tetracycline (each 14.3%). All strains were susceptible to chloramphenicol, cefoxitin, oxacillin, amikacin, and vancomycin. Three of seven strains displayed resistance to three or more antimicrobials. Resistance genes were found as follows: linA/linA' (100%), blaZ (85.7%), tet(K), ermC, ermT, and ermB (each 14.3%). Other resistant genes were not detected. In addition, the most frequently identified exotoxin genes were seu, lukED, hla, hlb, hld (each 100%), followed by hlg and hlgv (each 85.7%), lukPV (71.4%), sea (57.1%), see (42.9%), etd (28.6%), and seb, sec, sed, sej, and sek (each 14.3%). The results indicated that S. aureus ST6-t701, with a high prevalence in the northwest of China, exhibited multidrug resistance and harbored multiple toxin and resistance genes. Therefore, strict hygienic and preventative measures should be taken in order to avoid the contamination of food by S. aureus and toxin production in foods.
This paper addresses the practical problem of apportioning the value of land equitably between joint owners of developed property in China in a logical way. Allocation of land share by existing methods causes unfairness. A new concept, using the ratio of land floor value, is introduced and an alternative approach is thereby established. To find such ratios using market data, related statistical techniques and procedures are given. The discussion suggests that the approach is logical in theory and easy to use in practice.
Purpose: This study was aimed at exploring the trends of health status of medical college freshmen in 10 years (2005-2014), as a guide for improving the physical health conditions of University students. Methods: Freshman physical test data from 2005 to 2014 were collected, and their height, body mass index (BMI), lung capacity and long distance running times were analyzed. Results: Mean height of male students increased significantly from 172.08±5.56 cm in 2005 to 159.36±5.27 cm in 2014 (t=26.028,P<0.001), while that of female students increased significantly from 175.11±5.50 cm in 2005 to 162.66±5.03 cm in 2014 (t=28.291,P<0.001). BMI of male students increased significantly (t=6.224,P<0.001) within the same period, in contrast to that of the female students (t=7.458,P<0.001). In addition, mean BMI of male students were higher than that of female students (Z=29.760,P<0.001). Lung capacity showed a decline for both groups (male: t=12.944,P<0.001; female: t=29.489,P<0.001). Long–distance running times increased (male: t=21.833,P<0.001; female: t=18.717,P<0.001). The percentage of overweight and obese male students was significantly higher than that in female students (male: 12.73%; female: 4.84%), and both showed increasing trends (male: Z=9.056,P<0.001; female: Z=3.643,P<0.001). Non-compliance of lung capacity with long-distance running time increased over the years (male: Z=12.892,P<0.001; female: Z=19.148,P<0.001; male: Z=14.596,P<0.001; female: Z=8.209,P<0.001). Conclusion: Height of college freshmen showed an upward trend, but their physical health condition showed a downtrend within the period of study.
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