2005
DOI: 10.1158/0008-5472.can-04-2657
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Survivin-Responsive Conditionally Replicating Adenovirus Exhibits Cancer-Specific and Efficient Viral Replication

Abstract: Although a conditionally replicating adenovirus (CRA) exhibiting cancer-selective replication and induction of cell death is an innovative potential anticancer agent, current imperfections in cancer specificity and efficient viral replication limit the usefulness of this technique. Here, we constructed survivin-responsive CRAs (Surv.CRAs), in which expression of the wild-type or mutant adenoviral early region 1A (E1A) gene is regulated by the promoter of survivin, a new member of the inhibitor of apoptosis gen… Show more

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Cited by 57 publications
(83 citation statements)
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“…7,13,19,20 Reverse transcription-polymerase chain reaction analysis At 2 days after culturing cells with media in the presence or absence of 50 nM VD 3 (Sigma-Aldrich, Tokyo, Japan), total RNA was extracted from cells and reverse transcription-polymerase chain reaction analyses were performed using the primer sets and annealing temperatures that were described previously. 7,14,21 The osteocalcin/hypoxantine-phosphoribosyl-transferase (a representative housekeeping gene) mRNA ratio was calculated to compare the relative effects of VD 3 in individual cells and standardize the unavoidable variability in the polymerase chain reaction data as described. 21 …”
Section: Cells and Cell Culturementioning
confidence: 99%
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“…7,13,19,20 Reverse transcription-polymerase chain reaction analysis At 2 days after culturing cells with media in the presence or absence of 50 nM VD 3 (Sigma-Aldrich, Tokyo, Japan), total RNA was extracted from cells and reverse transcription-polymerase chain reaction analyses were performed using the primer sets and annealing temperatures that were described previously. 7,14,21 The osteocalcin/hypoxantine-phosphoribosyl-transferase (a representative housekeeping gene) mRNA ratio was calculated to compare the relative effects of VD 3 in individual cells and standardize the unavoidable variability in the polymerase chain reaction data as described. 21 …”
Section: Cells and Cell Culturementioning
confidence: 99%
“…[4][5][6] In the second category, E1 expression is altered in a cancer-specific manner because the promoter driving E1, particularly E1A, is replaced. 7 Although previous studies demonstrated that both strategies are efficacious, it is possible that a single cancer-specific factor will be insufficient to strictly target cancer, potentially leading to adverse side effects in future clinical trials. 8 A combination of multiple cancerspecific factors may be a promising approach to increase the safety of CRAs; 1,9,10 however, it is largely unclear whether and to what degree the anticancer effects are decreased by introducing multiple cancer-specific factors.…”
Section: Introductionmentioning
confidence: 99%
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