<i>TaRPP13‐3</i>, a CC‐NBS‐LRR‐like gene located on chr 7D, promotes disease resistance to wheat powdery mildew in Brock

Liu, Xiaoying; Zhang, Chaoqun; Zhang, Lulu; Huang, J. S.; Dang, Chen; Xie, Chaojie; Wang, Zhenying

doi:10.1111/jph.12949

Cited by 10 publications

(5 citation statements)

References 46 publications

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“…The R gene RPP13 encodes a protein containing a coiled-coil (CC) domain, nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) region. This gene has been associated with resistance to downy mildew in A. thaliana (Bittner-Eddy and Beynon, 2001) and wheat (Liu et al, 2020b). Most of the intracellular R gene encoding NBS and LRR domain belong to a superfamily of genes that provide resistance to pathogens through point mutations that led to the recognition of specific effectors proteins.…”

Section: Discussionmentioning

confidence: 99%

Association analysis of grain mould resistance in a core collection of NPGS Ethiopian sorghum germplasm

Cuevas,

Prom

2024

Plant Genet. Resour.

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The Ethiopian core collection of the USDA-Agriculture Research Service, National Plant Germplasm System (NPGS) includes 376 accessions and a genomic characterization that revealed 151,210 single nucleotide polymorphisms. This collection, however, lacks phenotypic characterization for several important agriculture traits. A total of 330 accessions from this NPGS Ethiopian core set were evaluated for grain mould resistance response across four tropical environments. Most of the accessions in the NPGS Ethiopian collection showed susceptibility to grain mould based on the low emergence rate and high seed deterioration observed in the seeds. The population structure of the collection was not related to grain mould resistance response suggesting this germplasm originated in regions with low disease pressure. The analysis identified two accessions with high emergence (PI 457867 and PI 454221) and three (PI 455036, PI 455213 and PI 330821) with low seed degradation. Genome-wide association analysis found genomic regions in chromosome 1, 3 and 8 associated with the observed grain mould resistance variation. Candidate gene analysis within these three loci identified diseases resistance genes involved in pathogen recognition and signalling cascades of the plant immunity system. These five NPGS Ethiopian accessions are candidates for use in a pre-breeding germplasm programme to develop improved germplasm with grain mould resistance.

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Section: Discussionmentioning

confidence: 99%

Association analysis of grain mould resistance in a core collection of NPGS Ethiopian sorghum germplasm

Cuevas,

Prom

2024

Plant Genet. Resour.

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“…The homologues of the RPP13 gene identified in other plant studies were found to play a key role in resistance to fungi, bacteria and viruses. RPP13-like genes differ in the position and peptide length of the LRR domains and participate in resistance of tomato to yellow leaf curl virus [ 42 ], grape powdery mildew [ 43 ], sugarcane leaf shedding [ 44 ], wheat powdery mildew [ 45 ] and barley powdery mildew [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5

Toporowska

Sowa

Kilian

et al. 2021

IJMS

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Crown rust, caused by Puccinia coronata f. sp. avenae, is one of the most destructive fungal diseases of oat worldwide. Growing disease-resistant oat cultivars is the preferred method of preventing the spread of rust and potential epidemics. The object of the study was Pc50-5, a race-specific seedling crown rust resistant gene, highly effective at all growth stages, selected from the differential line Pc50 (Avena sterilis L. CW 486-1 × Pendek). A comparison of crown rust reaction as well as an allelism test showed the distinctiveness of Pc50-5, whereas the proportions of phenotypes in segregating populations derived from a cross with two crown rust-susceptible Polish oat cultivars, Kasztan × Pc50-5 and Bingo × Pc50-5, confirmed monogenic inheritance of the gene, indicating its usefulness in oat breeding programs. Effective gene introgression depends on reliable gene identification in the early stages of plant development; thus, the aim of the study was to develop molecular markers that are tightly linked to Pc50-5. Segregating populations of Kasztan × Pc50-5 were genotyped using DArTseq technology based on next-generation Illumina short-read sequencing. Markers associated with Pc50-5 were located on chromosome 6A of the current version of the oat reference genome (Avena sativa OT3098 v2, PepsiCo) in the region between 434,234,214 and 440,149,046 bp and subsequently converted to PCR-based SCAR (sequence-characterized amplified region) markers. Furthermore, 5426978_SCAR and 24031809_SCAR co-segregated with the Pc50-5 resistance allele and were mapped to the partial linkage group at 0.6 and 4.0 cM, respectively. The co-dominant 58163643_SCAR marker was the best diagnostic and it was located closest to Pc50-5 at 0.1 cM. The newly discovered, very strong monogenic crown rust resistance may be useful for oat improvement. DArTseq sequences converted into specific PCR markers will be a valuable tool for marker-assisted selection in breeding programs.

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“…Recent studies have, however, identified Rpp13-like NLR genes associated with powdery mildew resistance in cereals. In wheat, Liu et al 83 showed that silencing of the Rpp13 homologous gene TaRPP13-3 in resistant wheat cv. ‘Brock’ induced susceptibility to powdery mildew.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a novel powdery mildew (Blumeria graminis) resistance locus in rye (Secale cereale L.)

Vendelbo

Mahmood²,

Sarup³

et al. 2021

Sci Rep

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Powdery mildew is one of the most destructive diseases in the world, causing substantial grain yield losses and quality reduction in cereal crops. At present 23 powdery mildew resistance genes have been identified in rye, of which the majority are in wheat-rye translocation lines developed for wheat improvement. Here, we investigated the genetics underlying powdery mildew resistance in the Gülzow-type elite hybrid rye (Secale cereale L.) breeding germplasm. In total, 180 inbred breeding lines were genotyped using the state-of-the-art 600 K SNP array and phenotyped for infection type against three distinct field populations of B. graminis f. sp. secalis from Northern Germany (2013 and 2018) and Denmark (2020). We observed a moderate level of powdery mildew resistance in the non-restorer germplasm population, and by performing a genome-wide association study using 261,406 informative SNP markers, we identified a powdery mildew resistance locus, provisionally denoted PmNOS1, on the distal tip of chromosome arm 7RL. Using recent advances in rye genomic resources, we investigated whether nucleotide-binding leucine-rich repeat genes residing in the identified 17 Mbp block associated with PmNOS1 on recent reference genomes resembled known Pm genes.

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TaRPP13‐3, a CC‐NBS‐LRR‐like gene located on chr 7D, promotes disease resistance to wheat powdery mildew in Brock

Cited by 10 publications

References 46 publications

Association analysis of grain mould resistance in a core collection of NPGS Ethiopian sorghum germplasm

Association analysis of grain mould resistance in a core collection of NPGS Ethiopian sorghum germplasm

Discovery and Chromosomal Location a Highly Effective Oat Crown Rust Resistance Gene Pc50-5

Discovery of a novel powdery mildew (Blumeria graminis) resistance locus in rye (Secale cereale L.)

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