<i>Taxus</i> ingredients in the red arils of <scp><i>Taxus baccata</i></scp> L. determined by HPLC‐MS/MS

Siegle, Lydia; Pietsch, Jörg

doi:10.1002/pca.2748

Cited by 16 publications

(15 citation statements)

References 39 publications

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“…UHPLC fingerprint analysis is currently the best choice ( Siegle and Pietsch, 2018 ). The original fingerprint chromatograms data in format (.lcd) of 19 batches of OH were exported as AIA files (*.cdf) and calculated using professional software [Similarity Evaluation System (SES) for Chromatographic Fingerprint of Traditional Chinese Medicine, composed by the Chinese Pharmacopoeia Committee (SES, Version 2012.130,723)].…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we attempted to detect and characterize the major constituents in OH using ultra high performance liquid chromatography–mass spectrometry (UHPLC-MS) ( Li et al, 2003 ; Li J. et al, 2018 ; Siegle and Pietsch, 2018 ) and UHPLC triple time-of-flight mass spectrometry (UHPLC-Triple-TOF-MS) ( Zhao et al, 2017 ; Huo et al, 2018 ), for identifying the major constituents of OH for the first time. By combining three identification techniques, namely, plant DNA barcoding ( Chen et al, 2010 ; Yao et al, 2010 ), thin-layer chromatography (TLC) ( Mustarichie et al, 2019 ; Ansari et al, 2020 ; Jakinala et al, 2021 ), and microscopic identification ( Li et al, 2015 ), we verified the different batches of OH, both authentic and inauthentic, collected from different provinces.…”

Section: Introductionmentioning

confidence: 99%

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The Analyses of Chemical Components From Oldenlandia hedyotidea (DC.) Hand.-Mazz and Anticancer Effects in vitro

et al. 2021

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Oldenlandia hedyotidea (DC.) Hand.-Mazz (OH), also known as sweet tea, is a valuable functional food with medicinal properties and is used for the treatment of cold, cough, gastroenteritis, heatstroke, herpes zoster, and rheumatoid arthritis. The phytochemicals in plant-based foods are responsible for the occurrence of these diseases to some extent. However, there is a scarcity of information on the chemical components of OH. We, therefore, aimed to investigate the phytochemical components of OH using ultra high-performance liquid chromatography–mass spectrometry (UHPLC-MS) and UHPLC triple time-of-flight mass spectrometry (UHPLC-Triple-TOF-MS). The main component of the OH extract, asperulosidic acid, was additionally quantified using UHPLC with ultraviolet detection (UHPLC-UV). The anticancer activity of the OH extract was assessed by a cell proliferation assay and a scratch assay using an esophageal cancer cell line. Ten compounds were tentatively identified in the aqueous extract of OH, including five iridoids, two anthraquinones, and one phenolic acid. The content of asperulosidic acid in the aqueous extract of OH was approximately 42 μg ml−1, and the extract exerted definite in vitro anticancer effects. The results can be used for quality control and assessment of the OH extract, which can serve as a promising source of functional ingredients for potential use in the food and drug industries.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Analyses of Chemical Components From Oldenlandia hedyotidea (DC.) Hand.-Mazz and Anticancer Effects in vitro

et al. 2021

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“…Not only taxol, but also some other taxoids have exhibited potent anti-tumor activities [10,11]. To date, more and more compounds with the taxane core have been isolated and identified in various Taxaceae trees [12]. Four novel taxane derivatives with high anticancer potency were isolated from the ethanol extract of the whole plant of T. wallichiana [13].…”

Section: Introductionmentioning

confidence: 99%

“…As one of the most important 13-hydroxylated taxoids, Taxol's specific synthetic branch involves 19 steps from the diterpenoid progenitor geranylgeranyl diphosphate (GGPP), which is derived via the plastidial 2-C-methyl-Derythritol phosphate (MEP) pathway, to taxol [9]. To form the taxane skeleton, cyclization of GGPP to taxa-4(5),11 (12)-diene is catalyzed by a slow-starter enzyme, taxadiene synthase (TS) [18]. Then, multiple modification enzymes, particularly from the CYP450, acyltransferase, aroyltransferase and benzoyltransferase families, participate in the structural modification of the skeleton [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Omic analysis of the endangered Taxaceae species Pseudotaxus chienii revealed the differences in taxol biosynthesis pathway between Pseudotaxus and Taxus yunnanensis trees

Zhang

et al. 2021

BMC Plant Biol

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Background Taxol is an efficient anticancer drug accumulated in Taxus species. Pseudotaxus chienii is an important member of Taxaceae, however, the level of six taxoids in P. chienii is largely unknown. Results High accumulation of 10-DAB, taxol, and 7-E-PTX suggested that P. chienii is a good taxol-yielding species for large-scale cultivation. By the omics approaches, a total of 3,387 metabolites and 61,146 unigenes were detected and annotated. Compared with a representative Taxus tree (Taxus yunnanensis), most of the differentially accumulated metabolites and differential expressed genes were assigned into 10 primary and secondary metabolism pathways. Comparative analyses revealed the variations in the precursors and intermediate products of taxol biosynthesis between P. chienii and T. yunnanensis. Taxusin-like metabolites highly accumulated in P. chienii, suggesting a wider value of P. chienii in pharmaceutical industry. Conclusions In our study, the occurrence of taxoids in P. chienii was determined. The differential expression of key genes involved in the taxol biosynthesis pathway is the major cause of the differential accumulation of taxoids. Moreover, identification of a number of differentially expressed transcription factors provided more candidate regulators of taxol biosynthesis. Our study may help to reveal the differences between Pseudotaxus and Taxus trees, and promote resource utilization of the endangered and rarely studied P. chienii.

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“…As one of the chromatographic separation modes, ultra‐performance liquid chromatography (UPLC) coupling with mass spectrometry (MS) has been adopted widely with rapid analysis time and excellent separation efficiency in qualitative and quantitative analysis of TCMs . UPLC‐MS improves analytical efficiency of chemical species because the system enhances the signal‐to‐noise ratio and minimises sample‐matrix effects, including ion suppression . Herein, in this study, the four related components, including isorhamnetin‐3‐ O ‐neohesperidoside, curcumin, peimine and tetrahydropalmatine (the chemical structures are shown in the Supporting Information Figure S1) were chosen for analysis via UPLC‐MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Application of UPLC‐MS/MS to simultaneously detect four bioactive compounds in the tumour‐shrinking decoction (FM1523) for uterine fibroids treatment

Cheng

Leung

et al. 2019

Phytochemical Analysis

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Introduction The Chinese medicine formulation, tumour‐shrinking decoction (TSD, FM1523), which consists of 15 natural medicines, is used for uterine fibroids (UFs) therapy and possesses excellent clinical therapeutic effect. Objective To develop a sensitive and validated analytical method for the simultaneous quantification of four crucial bioactive compounds including isorhamnetin‐3‐O‐neohesperidoside, curcumin, peimine and tetrahydropalmatine in the principal formulation of this decoction. Methods An ultra‐performance liquid chromatography coupled tandem mass spectrometry (UPLC‐MS/MS) with an electrospray ionisation (ESI) source in multiple reaction monitoring (MRM) mode was conducted to investigate these bioactive compounds in the TSD. The chromatographic separation was performed on a C18 column when the flow rate was adjusted at 0.2 mL/min with gradient elution of acetonitrile–water with 0.1% formic acid. Accelerated solvent extraction (ASE) method with higher extraction efficiency was employed for TSD sample pre‐treatment. Results The linearity, limit of detection (LOD) and limit of quantification (LOQ) were determined for this analytical method. The mean recoveries of the compounds were determined between 100.23% and 104.02% with satisfactory relative standard deviation (RSD) in the ranges of 2.65% to 3.81%. The precision was evaluated by intra‐day and inter‐day tests, which revealed RSD within the ranges of 1.21% to 2.14% and 1.24% to 2.32%, respectively. Conclusion The bioactive compounds of TSD samples were successfully quantified via UPLC‐MS/MS with MRM mode. This study could help to evaluate the pharmacokinetic study of TSD during clinical applications and present a facile strategy for quantifying bioactive compounds in traditional Chinese Medicine decoction.

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Taxus ingredients in the red arils of Taxus baccata L. determined by HPLC‐MS/MS

Abstract: The developed quantitative method is reliable and selective and was successfully applied for quantification of selected Taxus ingredients in red arils of Taxus baccata. It was disproved that the red arils of the berries do not contain the selected Taxus compounds.

Cited by 16 publications

References 39 publications

The Analyses of Chemical Components From Oldenlandia hedyotidea (DC.) Hand.-Mazz and Anticancer Effects in vitro

The Analyses of Chemical Components From Oldenlandia hedyotidea (DC.) Hand.-Mazz and Anticancer Effects in vitro

Omic analysis of the endangered Taxaceae species Pseudotaxus chienii revealed the differences in taxol biosynthesis pathway between Pseudotaxus and Taxus yunnanensis trees

Application of UPLC‐MS/MS to simultaneously detect four bioactive compounds in the tumour‐shrinking decoction (FM1523) for uterine fibroids treatment

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