<i>Testin</i> was regulated by circRNA3175‐miR182 and inhibited endometrial epithelial cell apoptosis in pre‐receptive endometrium of dairy goats

Zhang, Lei; Liu, Xiaorui; Ma, Xin; Liu, Yuexia; Che, Sicheng; Cui, Jiuzeng; An, Xiaopeng; Cao, Binyun; Song, Yuxuan

doi:10.1002/jcp.26614

Cited by 12 publications

(17 citation statements)

References 50 publications

(63 reference statements)

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“…Circular RNAs (circRNAs) can act as posttranscriptional regulators by sponging miRNAs (Memczak et al, 2013), and some circRNAs may have important roles in CEEC and RE establishment (L. L. Zhang, Liu, Ma, et al, 2018).…”

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confidence: 99%

miR‐34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ‐8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways

Liu

Zhang

Yang

et al. 2020

Journal Cellular Physiology

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microRNAs (miRNAs) and circular RNAs (circRNAs) are important for endometrial receptivity establishment and embryo implantation in mammals. miR‐34a and miR‐34c are highly expressed in caprine receptive endometrium (RE). Herein, the functions and mechanisms of miR‐34a/c in caprine endometrial epithelial cell (CEEC) apoptosis and RE establishment were investigated. miR‐34a/c downregulated the expression level of centrosomal protein 55 (CEP55) and were sponged by circRNA8073 (circ‐8073), thereby exhibiting a negative interaction in CEEC. miR‐34a/c induced CEEC apoptosis by targeting circ‐8073/CEP55 through the regulation of the RAS/RAF/MEK/ERK and phosphoitide 3‐kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathways. Positive and negative feedback loops and cross‐talk were documented between the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways. miR‐34a/c regulated the levels of RE marker genes, including forkhead box M1, vascular endothelial growth factor, and osteopontin (OPN). These results suggest that miR‐34a/c not only induce CEEC apoptosis by binding to circ‐8073 and CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways, but may also regulate RE establishment in dairy goats.

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confidence: 99%

miR‐34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ‐8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways

Liu

Zhang

Yang

et al. 2020

Journal Cellular Physiology

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“…Recently, circRNAs have also been suggested to constitute a subpopulation of ceRNAs that can act similar to a sponge and absorb miRNAs by base complementary pairing, thereby regulating the expression of miRNA target genes. For example, Zhang et al (2018) found in the prereceptive endometrium of dairy goats that ciR3175 can reduce the level of miR-182 by acting as a sponge of miRNA, and miR-182 can downregulate TES in endometrial epithelial cells (EECs) through the target site, as predicted, in vitro . In this way, ciR3175 acts as a ceRNA that isolates miR-182, thereby protecting TES transcripts from miR-182-mediated EEC inhibition in vitro .…”

Section: Competitive Endogenous Rna Mechanisms Of Actionmentioning

confidence: 77%

Progress on the Regulation of Ruminant Milk Fat by Noncoding RNAs and ceRNAs

Chen

et al. 2021

Front. Genet.

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Milk fat is not only a key factor affecting the quality of fresh milk but also a major target trait forbreeding. The regulation of milk fat involves multiple genes, network regulation and signal transduction. To explore recent discoveries of pathway regulation, we reviewed the published literature with a focus on functional noncoding RNAs and epigenetic regulation in ruminants. Results indicate that miRNAs play key roles in the regulation of milk fat synthesis and catabolism in ruminants. Although few data are available, merging evidence indicates that lncRNAs and circRNAs act on milk fat related genes through indirect action with microRNAs or RNAs in the ceRNA network to elicit positive effects on transcription. Although precise regulatory mechanisms remain unclear, most studies have focused on the regulation of the function of target genes through functional noncoding RNAs. Data to help identify factors that can regulate their own expression and function or to determine whether self-regulation involves positive and/or negative feedback are needed. Despite the growing body of research on the role of functional noncoding RNA in the control of ruminant milk fat, most data are still not translatable for field applications. Overall, the understanding of mechanisms whereby miRNA, lncRNA, circRNA, and ceRNA regulate ruminant milk fat remains an exciting area of research.

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“…Primary dairy goat endometrial epithelial cells (gEECs) were isolated and purified by digestion of trypsin, centrifugation, and difference in tempo adherence (L. Zhang et al, , ) and observed by a light microscope (X. Qi et al, ; X.F. Qi et al, ).…”

Section: Methodsmentioning

confidence: 99%

LncRNA882 regulates leukemia inhibitory factor (LIF) by sponging miR‐15b in the endometrial epithelium cells of dairy goat

Zhang

Liu

Cui

et al. 2018

Journal Cellular Physiology

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Despite the fact that long noncoding RNAs (lncRNAs) play roles in almost all biological processes, little is known about their biological function in the endometrium during the formation of endometrial receptivity. In this study, a comprehensive analysis of lncRNAs in goat endometrial tissues on Day 5 (prereceptive endometrium, PE) and Day 15 (receptive endometrium, RE) of pregnancy was performed by using RNA-Seq. As a result, 668 differentially expressed lncRNAs (DELs) were found between the PE and RE. Further study showed that lncRNA882, regulated by estrogen (E2) and progestin (P4), could act as competing endogenous RNAs (ceRNAs) for miR-15b, which inhibited the expression of transforming growth factor-b-activated kinase 1 binding protein 3 (TAB3) and then indirectly regulated the level of leukemia inhibitory factor (LIF). This was helpful for the formation of endometrial receptivity in dairy goats. In conclusion, we elucidated the endometrium lncRNA profiles of PE and RE in dairy goats; lncRNA882 acted as a ceRNA for miR-15b and then indirectly regulated the level of LIF in goat endometrial epithelium cells. Thus, this study helped us to better understand the molecular regulation of endometrial receptivity in dairy goats. K E Y W O R D Sleukemia inhibitory factor (LIF), endometrial epithelium cells (EECs), long noncoding RNA 882 (lncRNA882), miR-15b

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Testin was regulated by circRNA3175‐miR182 and inhibited endometrial epithelial cell apoptosis in pre‐receptive endometrium of dairy goats

Cited by 12 publications

References 50 publications

miR‐34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ‐8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways

miR‐34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ‐8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways

Progress on the Regulation of Ruminant Milk Fat by Noncoding RNAs and ceRNAs

LncRNA882 regulates leukemia inhibitory factor (LIF) by sponging miR‐15b in the endometrial epithelium cells of dairy goat

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