TGFβ1genetic variants are associated with an increased risk of acute brucellosis

Abstract

Our findings propose that TGFβ1 + 868 TT genotype and TT/GG diplotype may confer increased risk of brucellosis in the examined population.

Association between TGFβ1 polymorphisms and chronic hepatitis B infection in an Iranian population

“…(-509 C/T, +869C/T, and +11929C/T) Blood samples were collected by withdrawing5mL of venous blood into sterile EDTA-containing tubes. DNA was extracted by the salting-out method, as described previously 21,22 . The isolated DNA was examined by electrophoresis using a 1% agarose gel, quantified spectrophotometrically, and stored at −20 °C until further use.…”

HLA‐G gene 14‐bp deletion variant protects Iranian subjects against chronic hepatitis B infection

“…Blood samples were collected by withdrawal of 5 ml venous blood from each individual involved in this study into sterile EDTA‐containing tubes. DNA was extracted using salting‐out method as described previously Eskandari‐Nasab, Moghadampour, and Sepanj‐Nia (), Sepanjnia, Eskandari‐Nasab, Moghadampour, Tahmasebi, and Dahmardeh (). The quality of the isolated DNA was examined by electrophoresis on 1% agarose gel, quantified spectrophotometrically and stored at −20°C till further use.…”

Relationship between CD14-159C/T gene polymorphism and acute brucellosis risk

“…Demographic characteristics of patients and their clinical complications are shown in Table 1. Brucellosis was diagnosed according to the clinical manifestations (including fever, night sweating, weakness, malaise, weight loss, splenomegaly, lymphadenophathy, myalgia and arthralgia) and positive blood cultures as described previously [29][30][31][32]. The control group included healthy blood donors with no evidence of brucellosis and genetic disorders and matched for age, sex, and geographic area.…”

“…Serological tests, defined as wright titre 1/160 plus mercaptoethanol test 1/ 80 or coomb's wright 1/320 certified the brucellosis infection. The complete details of the organism identification methods have been described previously in papers by this research team [29][30][31][32].…”