<i>The Challenge</i>: Assessment of risks posed by systemic insecticides to hymenopteran pollinators: New perception when we move from laboratory via (semi-)field to landscape scale testing?

Heimbach, Fred; Schmuck, Richard A.; Grünewald, Bernd; Campbell, Peter J.; Sappington, Keith; Steeger, Thomas; Davies, Leo

doi:10.1002/etc.3631

Cited by 10 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Area-wide spraying of insecticides for insect pests or more specific applications containing an insect growth regulator may be even more harmful to insect pollinator species such as A . mellifera than the amounts of PPF delivered to the environment via an ADAM approach [ 9 , 30 , 31 ]. With any pesticidal approach, there are risks to non-target organisms and the risk may be acceptable in times of mosquito control need, particularly in association with disease outbreaks.…”

Section: Discussionmentioning

confidence: 99%

Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach

et al. 2021

View full text Add to dashboard Cite

The frequency of arboviral disease epidemics is increasing and vector control remains the primary mechanism to limit arboviral transmission. Container inhabiting mosquitoes such as Aedes albopictus and Aedes aegypti are the primary vectors of dengue, chikungunya, and Zika viruses. Current vector control methods for these species are often ineffective, suggesting the need for novel control approaches. A proposed novel approach is autodissemination of insect growth regulators (IGRs). The advantage of autodissemination approaches is small amounts of active ingredients compared to traditional insecticide applications are used to impact mosquito populations. While the direct targeting of cryptic locations via autodissemination seems like a significant advantage over large scale applications of insecticides, this approach could actually affect nontarget organisms by delivering these highly potent long lasting growth inhibitors such as pyriproxyfen (PPF) to the exact locations that other beneficial insects visit, such as a nectar source. Here we tested the hypothesis that PPF treated male Ae. albopictus will contaminate nectar sources, which results in the indirect transfer of PPF to European honey bees (Apis mellifera). We performed bioassays, fluorescent imaging, and mass spectrometry on insect and artificial nectar source materials to examine for intra- and interspecific transfer of PPF. Data suggests there is direct transfer of PPF from Ae. albopictus PPF treated males and indirect transfer of PPF to A. mellifera from artificial nectar sources. In addition, we show a reduction in fecundity in Ae. albopictus and Drosophila melanogaster when exposed to sublethal doses of PPF. The observed transfer of PPF to A. mellifera suggests the need for further investigation of autodissemination approaches in a more field like setting to examine for risks to insect pollinators.

show abstract

Section: Discussionmentioning

confidence: 99%

Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Extrapolation of laboratory data to forecast the situation in the field is still not a standard procedure and will need verification by testing under field conditions. Statistical analysis of data from semi-field and field studies seems to be a weak aspect for this approach [9]. Due to the realistic environmental context and the high complexity of semi-field and field studies, the precision (low variability and high repeatability) known from laboratory studies is difficult, maybe even impossible, to achieve [1,10].…”

Section: Introduction mentioning

confidence: 99%

Which Endpoints Can Be Reliably Assessed in Semi-field Pollinator Species Testing without Estimating False Positive or False Negative? MDD’s and Replicates Issue

Candolfi¹,

Bargen²,

Bocksch³

et al. 2018

JAST-A

View full text Add to dashboard Cite

Statistical power, number of replicates and experiment complexity of semi-field and field studies on Apis and non-Apis bee species has become a major issue after publication of the draft European Food Safety Authority (EFSA) Guidance on risk assessment of plant protection products (PPP) on bees (Apis mellifera, Bombus spp. and solitary bees). According to this guidance document, field studies have to be designed to be able to detect significance differences as low as 7% for certain endpoints such as reduction in colony size. This will require an immense number of replicates which is obviously not feasible. In the present study, key endpoints such as mortality, termination rate and number of brood cells in honeybee studies, cocoon production and flight activity in solitary bee studies and number of gynes in bumble bee studies (just to mention some of the endpoints considered) in semi-field studies were analyzed, with Apis mellifera, Bombus terrestris and Osmia bicornis during the past five years (2013-2017). The results indicate huge differences in the percentage minimal detectable differences (%MDDs) (MDD expressed as median of control value of the endpoint in percent) depending on endpoint and species tested. For honeybee semi-field studies, the lowest %MDDs recorded were between 10% and 15% for the endpoints foraging, number of brood cells and colony strength. The highest %MDDs were observed for the endpoint termination rate, with a %MDD of almost 50%. For the endpoints in bumble bee semi-field studies the %MDDs varied between 17% for bumble bee colony weight and 53% for average mortality during the exposure period in the tunnel. The %MDD for the number of gynes (young queens) was slightly below 25%. For the semi-field solitary bee test system, the %MDDs for the measured endpoints seem to be lower than those for the other two species tested. The %MDDs for the endpoints hatching of offspring, nest occupation and number of cocoons were 8%, 13% and 14%, respectively. Most of the %MDDs were between 10% and 30% indicating clearly that the currently performed experimental design for the semi-field pollinator studies allowed to determine relatively small effects on key study endpoints. The analysis indicated that for all the three bee species tested, the semi-field test design detected low %MDDs for most of the endpoints. It was also observed that detectable differences between the control and PPP treatments were much lower in semi-field test designs than in field studies with these bee species. The "perfect sample size" really does not exist but test design and statistical analysis can be adapted to lower the %MDDs. Measured and simulated (according to Student's t-test-distribution) data and results showed that statistical evaluations parameter selection (e.g., alpha value), data transformation (log10) and the number of replicates had a direct effect on the ability of the test design to detect lower or higher %MDD values. It could show that a change of alpha value from 0.05 to 0.1, increases the ability of the studie...

show abstract

Large-scale monitoring of effects of clothianidin-dressed oilseed rape seeds on pollinating insects in Northern Germany: justification of study design and statistical analysis

et al. 2017

View full text Add to dashboard Cite

The Challenge: Assessment of risks posed by systemic insecticides to hymenopteran pollinators: New perception when we move from laboratory via (semi-)field to landscape scale testing?

Cited by 10 publications

References 29 publications

Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach

Indirect transfer of pyriproxyfen to European honeybees via an autodissemination approach

Which Endpoints Can Be Reliably Assessed in Semi-field Pollinator Species Testing without Estimating False Positive or False Negative? MDD’s and Replicates Issue

Large-scale monitoring of effects of clothianidin-dressed oilseed rape seeds on pollinating insects in Northern Germany: justification of study design and statistical analysis

Contact Info

Product

Resources

About