<i>Tribolium</i>embryo morphogenesis

Benton, Matthew A.; Pavlopoulos, Anastasios

doi:10.4161/bioa.27815

Cited by 10 publications

(4 citation statements)

References 45 publications

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“…More systematic probing of the mechanical properties of gastrulating Tribolium embryos will be required to understand the source of the forces acting on the serosa. We expect that the condensation of the adjoining embryonic primordium 13 , 14 , together with additional forces generated by the attachment of the blastoderm to the vitelline envelope 30 , the yolk 13 , active crawling of the serosa on the vitelline envelope 12 , and regulated changes in the shape and stiffness of the serosal cells 17 , 34 , 35 , may also contribute to the serosal epiboly.…”

Section: Discussionmentioning

confidence: 99%

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Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation

et al. 2020

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Many animal embryos pull and close an epithelial sheet around the ellipsoidal egg surface during a gastrulation process known as epiboly. The ovoidal geometry dictates that the epithelial sheet first expands and subsequently compacts. Moreover, the spreading epithelium is mechanically stressed and this stress needs to be released. Here we show that during extraembryonic tissue (serosa) epiboly in the insect Tribolium castaneum, the non-proliferative serosa becomes regionalized into a solid-like dorsal region with larger non-rearranging cells, and a more fluid-like ventral region surrounding the leading edge with smaller cells undergoing intercalations. Our results suggest that a heterogeneous actomyosin cable contributes to the fluidization of the leading edge by driving sequential eviction and intercalation of individual cells away from the serosa margin. Since this developmental solution utilized during epiboly resembles the mechanism of wound healing, we propose actomyosin cable-driven local tissue fluidization as a conserved morphogenetic module for closure of epithelial gaps.

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Section: Discussionmentioning

confidence: 99%

“…1b ). The spreading serosal tissue expands over the posterior pole and eventually closes ventrally over the contracting embryo in a process known as serosa window closure 12 – 14 . However, it is not understood how the leading serosal cells at the rim of the serosa window achieve final compaction.…”

Section: Introductionmentioning

confidence: 99%

Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation

et al. 2020

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“…Tribolium castaneum has also been a popular model for studying developmental and evolutionary biology (18,34,85,131). Like other coleopterans, T. castaneum develops pleuropodia, A1 appendages that have been conserved among most insect orders, but lacks larval appendages on the more posterior abdominal segments A2-A8 (84).…”

Section: Geneticsmentioning

confidence: 99%

Tribolium castaneum: A Model Insect for Fundamental and Applied Research

Campbell

Athanassiou

Hagstrum

et al. 2022

Annu. Rev. Entomol.

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Tribolium castaneum has a long history as a model species in many distinct subject areas, but improved connections among the genetics, genomics, behavioral, ecological, and pest management fields are needed to fully realize this species’ potential as a model. Tribolium castaneum was the first beetle whose genome was sequenced, and a new genome assembly and enhanced annotation, combined with readily available genomic research tools, have facilitated its increased use in a wide range of functional genomics research. Research into T. castaneum’s sensory systems, response to pheromones and kairomones, and patterns of movement and landscape utilization has improved our understanding of behavioral and ecological processes. Tribolium castaneum has also been a model in the development of pest monitoring and management tactics, including evaluation of insecticide resistance mechanisms. Application of functional genomics approaches to behavioral, ecological, and pest management research is in its infancy but offers a powerful tool that can link mechanism with function and facilitate exploitation of these relationships to better manage this important food pest. Expected final online publication date for the Annual Review of Entomology, Volume 67 is January 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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“…When GBE occurs in Drosophila , the germband stays at the surface of the egg and the amnioserosa largely remains in place. In Tribolium , on the other hand, GBE begins with a process called embryo condensation, during which the embryonic ectoderm and presumptive amnion (together termed the ‘germ rudiment’) form the germband (see Fig 1 ; for a detailed description, see [ 14 , 28 ]). Several concurrent morphogenetic events underlie embryo condensation.…”

Section: Introductionmentioning

confidence: 99%

A revised understanding of Tribolium morphogenesis further reconciles short and long germ development

Benton

2018

PLoS Biol

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In Drosophila melanogaster, the germband forms directly on the egg surface and solely consists of embryonic tissue. In contrast, most insect embryos undergo a complicated set of tissue rearrangements to generate a condensed, multilayered germband. The ventral side of the germband is embryonic, while the dorsal side is thought to be an extraembryonic tissue called the amnion. While this tissue organisation has been accepted for decades and has been widely reported in insects, its accuracy has not been directly tested in any species. Using live cell tracking and differential cell labelling in the short germ beetle Tribolium castaneum, I show that most of the cells previously thought to be amnion actually give rise to large parts of the embryo. This process occurs via the dorsal-to-ventral flow of cells and contributes to germband extension (GBE). In addition, I show that true ‘amnion’ cells in Tribolium originate from a small region of the blastoderm. Together, my findings show that development in the short germ embryos of Tribolium and the long germ embryos of Drosophila is more similar than previously proposed. Dorsal-to-ventral cell flow also occurs in Drosophila during GBE, and I argue that the flow is driven by a conserved set of underlying morphogenetic events in both species. Furthermore, the revised Tribolium fate map that I present is far more similar to that of Drosophila than the classic Tribolium fate map. Lastly, my findings show that there is no qualitative difference between the tissue structure of the cellularised blastoderm and the short/intermediate germ germband. As such, the same tissue patterning mechanisms could function continuously throughout the cellularised blastoderm and germband stages, and easily shift between them over evolutionary time.

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Triboliumembryo morphogenesis

Cited by 10 publications

References 45 publications

Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation

Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation

Tribolium castaneum: A Model Insect for Fundamental and Applied Research

A revised understanding of Tribolium morphogenesis further reconciles short and long germ development

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