<i>Trichoderma harzianum</i> cerato‐platanin enhances hydrolysis of lignocellulosic materials

Pennacchio, Anna; Pitocchi, Rossana; Varese, Giovanna Cristina; Giardina, Paola; Piscitelli, Alessandra

doi:10.1111/1751-7915.13836

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…Studies demonstrated that CPs could bind to and weaken lignocellulosic materials, acting similarly to plant expansins and facilitating access to cellulolytic enzymes [50,51]. This characteristic has been reported in the CP of Ceratocystis populicola [51], Moniliophthora perniciosa [52], Fusarium graminearum [53], and Trichoderma harzianum [54]. In T. reesei, CPs were found in the secretome during fermentation processes in sugarcane bagasse [55].…”

Section: Discussionmentioning

confidence: 81%

The Cerato-Platanin EPL2 from Trichoderma reesei Is Not Directly Involved in Cellulase Formation but in Cell Wall Remodeling

Silva,

Oshiquiri,

de Morais Costa de Jesus

et al. 2023

Microorganisms

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Trichoderma reesei is a saprophytic fungus that produces large amounts of cellulases and is widely used for biotechnological applications. Cerato-platanins (CPs) are a family of proteins universally distributed among Dikarya fungi and have been implicated in various functions related to fungal physiology and interaction with the environment. In T. reesei, three CPs are encoded in the genome: Trire2_111449, Trire2_123955, and Trire2_82662. However, their function is not fully elucidated. In this study, we deleted the Trire2_123955 gene (named here as epl2) in the wild-type QM6aΔtmus53Δpyr4 (WT) strain and examined the behavior of the Δepl2 strain compared with WT grown for 72 h in 1% cellulose using RNA sequencing. Of the 9143 genes in the T. reesei genome, 760 were differentially expressed, including 260 only in WT, 214 only in Δepl2, and 286 in both. Genes involved in oxidative stress, oxidoreductase activity, antioxidant activity, and transport were upregulated in the Δepl2 mutant. Genes encoding cell wall synthesis were upregulated in the mutant strain during the late growth stage. The Δepl2 mutant accumulated chitin and glucan at higher levels than the parental strain and was more resistant to cell wall stressors. These results suggest a compensatory effect in cell wall remodeling due to the absence of EPL2 in T. reesei. This study is expected to contribute to a better understanding of the role of the EPL2 protein in T. reesei and improve its application in biotechnological fields.

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Section: Discussionmentioning

confidence: 81%

The Cerato-Platanin EPL2 from Trichoderma reesei Is Not Directly Involved in Cellulase Formation but in Cell Wall Remodeling

Silva,

Oshiquiri,

de Morais Costa de Jesus

et al. 2023

Microorganisms

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“…These data are of particular interest to optimize the pretreatment conditions, whose impact on enzymatic hydrolysis is not merely related to the degree of delignification [4]. In some cases, positive impact of pretreatment method is due to the superficial alteration that some solvent/catalysts (e.g., surfactants) can provide to the lignin fraction of the biomass [5,6].…”

mentioning

confidence: 99%

Effect of enzymes adsorption on enzymatic hydrolysis of coffee silverskin: Kinetic characterization and validation

Russo¹,

Procentese

Montagnaro

et al. 2022

Biochemical Engineering Journal

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Enzymatic hydrolysis of bacterial cellulose in the presence of a non‐catalytic cerato‐platanin protein

Rovera

Luti

Pazzagli

et al. 2021

J of Applied Polymer Sci

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In this work, the effect of an expansin-like cerato-platanin (CP) protein as a pre-treatment for the enzymatic hydrolysis of bacterial cellulose (BC) is investigated. To this scope, cellulases from Trichoderma reesei are used as hydrolyzing agent using different enzyme/BC formulations. Turbidity experiments reveal that for the higher enzyme concentrations (formulations 0.5:1 and 1:1) the enzymatic hydrolysis of BC show similar hydrolysis kinetics and is not dependent of the CP. However, at higher BC concentrations (formulations 0.25:1 and 0.33:1), the hydrolysis of BC is hindered by the non-catalytic protein, as confirmed by the lower content of cellobiose and glucose in the presence of CP. Light scattering experiments show that the addition of CP led to an increase of the BC particle size from (445-630 nm) to (890-1.26 μm) for the formulation 1:1, which is also corroborated by atomic force microscopy and transmission electron microscopy analyses. These results suggest that CP did not positively affect the hydrolysis of BC, in contrast to what was previously observed for plant-derived cellulose. This work for the first time investigates the anomalous behavior of cerato platanin family members with regard to its loosening activity on the structure of bacterial cellulose.

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Trichoderma harzianum cerato‐platanin enhances hydrolysis of lignocellulosic materials

Cited by 6 publications

References 27 publications

The Cerato-Platanin EPL2 from Trichoderma reesei Is Not Directly Involved in Cellulase Formation but in Cell Wall Remodeling

The Cerato-Platanin EPL2 from Trichoderma reesei Is Not Directly Involved in Cellulase Formation but in Cell Wall Remodeling

Effect of enzymes adsorption on enzymatic hydrolysis of coffee silverskin: Kinetic characterization and validation

Enzymatic hydrolysis of bacterial cellulose in the presence of a non‐catalytic cerato‐platanin protein

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