<i>Trichoderma virens</i> colonization of maize roots triggers rapid accumulation of 12-oxophytodienoate and two ᵧ-ketols in leaves as priming agents of induced systemic resistance

Wang, Ken-Der; Gorman, Zachary; Huang, Pei‐Cheng; Kenerley, Charles M.; Kolomiets, Michael V.

doi:10.1080/15592324.2020.1792187

Cited by 22 publications

(24 citation statements)

References 22 publications

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“…Despite that we noted the enhanced resistance of maize stems to GSR by exogenously applied MeJA, this effect is likely due to the induction of oxylipins other than JA. MeJAinducible resistance to GSR is likely independent of COI1a, or due to the yet unknown JA-independent defense pathways induced by MeJA, such as 9-LOX signaling pathways as shown recently for a 9-oxylipin alfa-ketol KODA of gamma-ketols [27,28]. Another candidate molecule is probably 12-OPDA that was recently shown to induce resistance to other hemibiotrophic pathogens [27] (Figure 7).…”

Section: Maize Susceptibility To F Graminearum Is Promoted By Ja and Coi1amentioning

confidence: 79%

“…[18] and Fusarium verticillioides [20], but resistant to anthracnose leaf blight and stalk rot, caused by a hemibiotrophic pathogen Colletotrichum graminicola [21]. Although underappreciated previously, 12-OPDA has been increasingly drawing attention as having functions distinct from those of JAs [22][23][24][25][26][27]. Particularly, a recent study revealed that 12-OPDA and α-ketol of octadecadienoic acid (KODA), but not JA, serve as the mobile signals in Trichodema-elicited induced systemic resistance (ISR) [28].…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, a recent study revealed that 12-OPDA and α-ketol of octadecadienoic acid (KODA), but not JA, serve as the mobile signals in Trichodema-elicited induced systemic resistance (ISR) [28]. Moreover, while exogenous treatment of maize with MeJA results in increased susceptibility to C. graminicola [21], application of 12-OPDA results in increased resistance to this pathogen [27].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Characterization of Jasmonates Signaling Components Reveals the Essential Role of ZmCOI1a-ZmJAZ15 Action Module in Regulating Maize Immunity to Gibberella Stalk Rot

Sun

Ruan

et al. 2021

IJMS

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Gibberella stalk rot (GSR) by Fusarium graminearum causes significant losses of maize production worldwide. Jasmonates (JAs) have been broadly known in regulating defense against pathogens through the homeostasis of active JAs and COI-JAZ-MYC function module. However, the functions of different molecular species of JAs and COI-JAZ-MYC module in maize interactions with Fusarium graminearum and regulation of diverse metabolites remain unknown. In this study, we found that exogenous application of MeJA strongly enhanced resistance to GSR. RNA-seq analysis showed that MeJA activated multiple genes in JA pathways, which prompted us to perform a genome-wide screening of key JA signaling components in maize. Yeast Two-Hybrid, Split-Luciferase, and Pull-down assays revealed that the JA functional and structural mimic coronatine (COR) functions as an essential ligand to trigger the interaction between ZmCOIa and ZmJAZ15. By deploying CRISPR-cas9 knockout and Mutator insertional mutants, we demonstrated that coi1a mutant is more resistant, whereas jaz15 mutant is more susceptible to GSR. Moreover, JA-deficient opr7-5opr8-2 mutant displayed enhanced resistance to GSR compared to wild type. Together, these results provide strong evidence that ZmJAZ15 plays a pivotal role, whereas ZmCOIa and endogenous JA itself might function as susceptibility factors, in maize immunity to GSR.

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Section: Maize Susceptibility To F Graminearum Is Promoted By Ja and Coi1amentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Characterization of Jasmonates Signaling Components Reveals the Essential Role of ZmCOI1a-ZmJAZ15 Action Module in Regulating Maize Immunity to Gibberella Stalk Rot

Sun

Ruan

et al. 2021

IJMS

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“…Only 13-hydroxy-10-oxo-(11E,15Z)-octadecadienoic acid (13,10-KODA) did not come close to statistical difference at this timepoint, but was significantly higher in PLV-treated plants at 4 dpi. Importantly, we have previously shown that exogenous treatments with several of these ketols, including the α-ketol 9,10-KODA (Wang et al, 2020a), and the γ-ketols, 9,12-KOMA and 9,12-KODA (Wang et al, 2020b), strongly increase maize resistance to C. graminicola.…”

Section: Plv-mediated Resistance To C Graminicola Correlates With Increased Synthesis Of Oxylipin Hydroxides and αAnd γ-Ketolsmentioning

confidence: 99%

The Synthesis of Pentyl Leaf Volatiles and Their Role in Resistance to Anthracnose Leaf Blight

et al. 2021

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Volatiles are important airborne chemical messengers that facilitate plant adaptation to a variety of environmental challenges. Lipoxygenases (LOXs) produce a bouquet of non-volatile and volatile oxylipins, including C6 green leaf volatiles (GLVs), which are involved in a litany of plant physiological processes. GLVs are emitted by a diverse array of plant species, and are the best-known group of LOX-derived volatiles. Five-carbon pentyl leaf volatiles (PLVs) represent another widely emitted group of LOX-derived volatiles that share structural similarity to GLVs, however, relatively little is known about their biosynthesis or biological activity. In this study, we utilized PLV-deficient mutants of maize and Arabidopsis and exogenous PLV applications to elucidate the biosynthetic order of individual PLVs. We further measured PLVs and GLVs after tissue disruption of leaves by two popular methods of volatile elicitation, wounding and freeze-thawing. Freeze-thawing distorted the volatile metabolism of both GLVs and PLVs relative to wounding, though this distortion differed between the two groups of volatiles. These results suggest that despite the structural similarity of these two volatile groups, they are differentially metabolized. Collectively, these results have allowed us to produce the most robust PLV pathway to date. To better elucidate the biological activity of PLVs, we show that PLVs induce maize resistance to the anthracnose pathogen, Colletotrichum graminicola, the effect opposite to that conferred by GLVs. Further analysis of PLV-treated and infected maize leaves revealed that PLV-mediated resistance is associated with early increases of oxylipin α- and γ-ketols, and later increases of oxylipin ketotrienes, hydroxytrienes, and trihydroxydienes. Ultimately, this study has produced the most up-to-date pathway for PLV synthesis, and reveals that PLVs can facilitate pathogen resistance through induction of select oxylipins.

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“…Furthermore, Trichoderma spp. induce the expression of genes related to defense, such as the plant defensin 1.2 gene (PDF1.2) and the pathogenesis-related 1a gene (PR-1a), which are used as markers for ISR-priming and SAR, respectively [19,[22][23][24][25]. In addition, some proteins of Trichoderma spp.…”

Section: Introductionmentioning

confidence: 99%

Secretome Analysis of Arabidopsis–Trichoderma atroviride Interaction Unveils New Roles for the Plant Glutamate:Glyoxylate Aminotransferase GGAT1 in Plant Growth Induced by the Fungus and Resistance against Botrytis cinerea

González-López

Jijón-Moreno

Dautt-Castro

et al. 2021

IJMS

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The establishment of plant–fungus mutualistic interaction requires bidirectional molecular crosstalk. Therefore, the analysis of the interacting organisms secretomes would help to understand how such relationships are established. Here, a gel-free shotgun proteomics approach was used to identify the secreted proteins of the plant Arabidopsis thaliana and the mutualistic fungus Trichoderma atroviride during their interaction. A total of 126 proteins of Arabidopsis and 1027 of T. atroviride were identified. Among them, 118 and 780 were differentially modulated, respectively. Bioinformatic analysis unveiled that both organisms’ secretomes were enriched with enzymes. In T. atroviride, glycosidases, aspartic endopeptidases, and dehydrogenases increased in response to Arabidopsis. Additionally, amidases, protein-serine/threonine kinases, and hydro-lyases showed decreased levels. Furthermore, peroxidases, cysteine endopeptidases, and enzymes related to the catabolism of secondary metabolites increased in the plant secretome. In contrast, pathogenesis-related proteins and protease inhibitors decreased in response to the fungus. Notably, the glutamate:glyoxylate aminotransferase GGAT1 was secreted by Arabidopsis during its interaction with T. atroviride. Our study showed that GGAT1 is partially required for plant growth stimulation and on the induction of the plant systemic resistance by T. atroviride. Additionally, GGAT1 seems to participate in the negative regulation of the plant systemic resistance against B. cinerea through a mechanism involving H2O2 production.

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Trichoderma virens colonization of maize roots triggers rapid accumulation of 12-oxophytodienoate and two ᵧ-ketols in leaves as priming agents of induced systemic resistance

Abstract: Trichodermavirens colonization of maize roots triggers rapid accumulation of 12-oxophytodienoate and two ᵧ-ketols in leaves as priming agents of induced systemic resistance,

Cited by 22 publications

References 22 publications

Genome-Wide Characterization of Jasmonates Signaling Components Reveals the Essential Role of ZmCOI1a-ZmJAZ15 Action Module in Regulating Maize Immunity to Gibberella Stalk Rot

Genome-Wide Characterization of Jasmonates Signaling Components Reveals the Essential Role of ZmCOI1a-ZmJAZ15 Action Module in Regulating Maize Immunity to Gibberella Stalk Rot

The Synthesis of Pentyl Leaf Volatiles and Their Role in Resistance to Anthracnose Leaf Blight

Secretome Analysis of Arabidopsis–Trichoderma atroviride Interaction Unveils New Roles for the Plant Glutamate:Glyoxylate Aminotransferase GGAT1 in Plant Growth Induced by the Fungus and Resistance against Botrytis cinerea

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