Vibrio bacteria isolated from black tiger shrimp, Penaeus monodon Fabricius

Abstract

White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.

Vibrio alginolyticus associated with white spot disease of Penaeus monodon

“…It has previously been reported that shrimp affected by WSSV become susceptible to invasion by environmental Vibrio species including V. alginolyticus (Karunasagar et al 1997). V. alginolyticus has also been reported as a pathogen associated with shrimp diseases including 'black splinter disease' in Thailand (Ruangpan & Kitao 1991). According to Ramasamy et al (1995), mortality in Penaeus monodon infected with monodon baculovirus (MBV) was hastened by secondary bacteria.…”

The Potential of Galangal (Alpinia galanga Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei)

“…vulnificus, V. alginolyticus, V. mimicus, and V. harveyi were isolated from diseased Pacific white shrimp (L. vannamei) obtained from grow-out ponds in Chanthaburi Province, using the Ruangpan & Kitao (1991) method. The isolate was streaked on Thiosulfate Citrate-Bilesalt Sucrose Agar (TCBS Agar, Difco USA) for selective isolation of Vibrio, and cultivated at 30 ºC for 18-24 h. The Vibrio species was identified according to a scheme developed by Colwell (1984) using API 20E kits (ATB System, BioMérieux, France) and the PCR technique for the detection of V. parahaemolyticus (EMS/AHPND) (Joshi et al, 2014).…”

Evaluation of the vaccine potential of a cytotoxic protease and a protective immunogen from a pathogenic Vibrio harveyi strain

“…For aquaculture industries, V. harveyi is considered as a severe opportunistic pathogen that can infect a wide range of marine species, including both vertebrates and invertebrates [1,2]. Penaeid shrimp is particularly susceptible to V. harveyi, and, upon V. harveyi infection, develops a disease called luminous vibriosis that can result in heavy economic losses [3][4][5][6][7][8]. In addition to shrimp, fish, lobster, and abalone are also known to be affected by V. harveyi-related vibriosis [9][10][11][12].…”