Xanthomonas arboricola pv. juglandis and pv. corylina: Brothers or distant relatives? Genetic clues, epidemiology, and insights for disease management

Kałużna, Monika; Saux, Marion Fischer-Le; Pothier, Joël F.; Jacques, Marie‐Agnès; Obradović, Aleksa; Tavares, Fernando; Stefani, Emilio

doi:10.1111/mpp.13073

Cited by 24 publications

(31 citation statements)

References 102 publications

(274 reference statements)

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“…gardneri, X. perforans and X. vesicatoria) still require several steps-observation of disease symptoms, microscopic examination, pathogen isolation, pathogenicity tests and molecular tests [27][28][29][30][31][32][33][34]. Having in mind that only recently X. euroxanthea was proposed as a new species [4], isolated from different plant hosts [7][8][9][10], for which the nature of the bacteria-plant interaction is still unknown, and no distinct symptoms have been described [4], the herein proposed multiplex PCR is the tool available for accurate detection and identification of X. euroxanthea.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, apart from its occurrence in walnuts (Juglans regia), recent evidence was gathered reporting the isolation of X. euroxanthea from distinct plant host species, such as Carya illinoensis (pecan; strains CPBF 761 and CPBF 766), that together with walnut (Juglans regia; strains CPBF 367, CPBF 424 T , CPBF 426 and CFBP 7653) belong to the Juglandaceae family; Solanum lycopersicum (tomato plants; strains BRIP 62409, BRIP 62411, BRIP 62415 and BRIP 62418) a member of the Solanaceae family [7][8][9]; and Phaseolus vulgaris (common bean; strain CFBP 7622, previously misclassified as X. arboricola [10,11]), a Fabaceae plant species.…”

Section: Introductionmentioning

confidence: 99%

“…Altogether, the common ecological niche of these two closely related species, plus their cosmopolitan distribution and co-occurrence in different host plant species resulted in misclassifying some X. euroxanthea isolates as X. arboricola [8,10,11], which calls for the need to develop methods for the detection and genotyping of this bacterial species. Over the years, different diagnostic and molecular typing tools have been developed for a variety of xanthomonads [13], namely for X. arboricola including their most studied pathovars juglandis [14,15] and pruni [16][17][18][19], aiming to address their diversity within a geographic or epidemiological context.…”

Section: Introductionmentioning

confidence: 99%

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DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea

et al. 2022

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Xanthomonas euroxanthea is a bacterial species encompassing both pathogenic and non-pathogenic strains and is frequently found colonizing the same host plants as X. arboricola. This presents the need to develop a detection and genotyping assay able to track these bacteria in microbial consortia with other xanthomonads. Eight X. euroxanthea-specific DNA markers (XEA1-XEA8) were selected by comparative genomics and validated in silico regarding their specificity and consistency using BLASTn, synteny analysis, CG content, codon usage (CAI/eCAI values) and genomic proximity to plasticity determinants. In silico, the selected eight DNA markers were found to be specific and conserved across the genomes of 11 X. euroxanthea strains, and in particular, five DNA markers (XEA4, XEA5, XEA6, XEA7 and XEA8) were unfailingly found in these genomes. A multiplex of PCR targeting markers XEA1 (819 bp), XEA8 (648 bp) and XEA5 (295 bp) was shown to successfully detect X. euroxanthea down to 1 ng of DNA (per PCR reaction). The topology of trees generated with the concatenated sequences of three markers (XEA5, XEA6 and XEA8) and four housekeeping genes (gyrB, rpoD, fyuA and acnB) underlined the equal discriminatory power of these features and thus the suitability of the DNA markers to discriminate X. euroxanthea lineages. Overall, this study displays a DNA-marker-based method for the detection and genotyping of X. euroxanthea strains, contributing to monitoring for its presence in X. arboricola-colonizing habitats. The present study proposes a workflow for the selection of species-specific detection markers. Prospectively, this assay could contribute to unveil alternative host species of Xanthomonas euroxanthea; and improve the control of phytopathogenic strains.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea

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“…The resulting disease susceptibility on the different species was assessed using the percentage of seedlings with cankers on the stems and branches. In a recent review, Kałuzna et al (2021) highlighted the similarities between Xac and Xaj suggesting that inoculation, identification, and management techniques successful on Xaj could also be informative if applied on Xac. The time saved by using this spray inoculation technique would allow for greater population sizes and could be incorporated into mass bacterial blight screening on hazelnut.…”

Section: Discussionmentioning

confidence: 99%

“…(Miller et al, 1940). Bacterial blight symptoms are found on leaves, buds, twigs, trunks, and occasionally nuts, primarily on young hazelnut trees between 1 and 4 years old (Miller et al, 1949;Scortichini et al, 2002;Lamichhane and Varvaro, 2014;Kałuzna et al, 2021). It has been shown that Xac may reside epiphytically on asymptomatic plant tissues including under bud scales for extended periods without inducing symptoms (Pisetta et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Some Corylus avellana L. Cultivars to Xanthomonas arboricola pv. corylina

et al. 2021

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Bacterial blight of hazelnut (Corylus avellana L.) is caused by Xanthomonas arboricola pv. corylina (Xac). In the past, bacterial blight has been a key disease impacting the Oregon hazelnut industry where 99% of the United States hazelnut crop is grown. The disease is re-emerging in young orchards, as acreage of newly released hazelnut cultivars rapidly increases. This increase in hazelnut acreage is accompanied by renewed interest in developing control strategies for bacterial blight. Information on susceptibility of hazelnut cultivars to Xac is limited, partially due to lack of verified methods to quantify hazelnut cultivar response to artificial inoculation. In this research, Xac inoculation protocols were adapted to two hazelnut growing environments to evaluate cultivar susceptibility: in vitro tissue culture under sterile and controlled conditions, and in vivo potted tree conditions. Five hazelnut cultivars were evaluated using the in vitro inoculation protocol and seven hazelnut cultivars were evaluated using the in vivo inoculation protocol. Under in vitro conditions, there were severe bacterial blight symptoms on each cultivar consistent with those seen in the field, but no significant differences in the susceptibility of the newly released cultivars were observed compared to known Xac-susceptible cultivar (“Barcelona”). Under in vivo conditions, the proportion of necrotic buds were significantly higher in “Jefferson” and “Dorris” compared to all of the other tested cultivars, including “Barcelona.” The symptom progression seen in vivo mirrored the timing and symptom progression of bacterial blight reported from field observations. The in vitro conditions significantly reduced the amount of time required to measure the inoculation efficiency compared to the in vivo environment and allowed for greater replication. Further studies on the effects of Xac can use the results of these experiments to establish a dose–response model for bacterial blight, a wider range of germplasm can be tested under in vitro conditions, and management strategies that can be evaluated on large populations of new cultivars using the in vivo methods.

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Population characteristics of Xanthomonas arboricola pv. corylina strains from hazelnut orchards in Turkey

Öztürk

2023

J Plant Dis Prot

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Xanthomonas arboricola pv. juglandis and pv. corylina: Brothers or distant relatives? Genetic clues, epidemiology, and insights for disease management

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 24 publications

References 102 publications

DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea

DNA Markers for Detection and Genotyping of Xanthomonas euroxanthea

Susceptibility of Some Corylus avellana L. Cultivars to Xanthomonas arboricola pv. corylina

Population characteristics of Xanthomonas arboricola pv. corylina strains from hazelnut orchards in Turkey

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