<i>Xenopus frizzled‐7</i> morphant displays defects in dorsoventral patterning and convergent extension movements during gastrulation

Sumanas, Saulius; Ekker, Stephen C.

doi:10.1002/gene.1044

Cited by 41 publications

(30 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inhibition of canonical Wnt signalling by a dominant-negative form of Tcf3 does not inhibit neural crest migration (F. Romero and R.M., unpublished). There is convincing experimental evidence that shows that canonical Wnt signalling is involved in neural crest induction and cell differentiation (Dorsky et al, 1998;Garcia-Castro et al, 2002;LaBonne and Bronner-Fraser, 1998;Lee et al, 2004 (CarreiraBarbosa et al, 2003;Djiane et al, 2000;Kuhl et al, 2000;Medina et al, 2000;Sumanas and Ekker, 2001;Wang and Malbon, 2004;Winklbauer et al, 2001). We have shown that Fz7 is expressed in the premigratory neural crest just before migration, as well as in the migrating neural crest.…”

Section: Discussionmentioning

confidence: 50%

See 1 more Smart Citation

Essential role of non-canonical Wnt signalling in neural crest migration

et al. 2005

View full text Add to dashboard Cite

Migration of neural crest cells is an elaborate process that requires the delamination of cells from an epithelium and cell movement into an extracellular matrix. In this work, it is shown for the first time that the non-canonical Wnt signalling [planar cell polarity (PCP) or Wnt-Ca2+] pathway controls migration of neural crest cells. By using specific Dsh mutants, we show that the canonical Wnt signalling pathway is needed for neural crest induction, while the non-canonical Wnt pathway is required for neural crest migration. Grafts of neural crest tissue expressing non-canonical Dsh mutants, as well as neural crest cultured in vitro, indicate that the PCP pathway works in a cell-autonomous manner to control neural crest migration. Expression analysis of non-canonical Wnt ligands and their putative receptors show that Wnt11 is expressed in tissue adjacent to neural crest cells expressing the Wnt receptor Frizzled7 (Fz7). Furthermore, loss- and gain-of-function experiments reveal that Wnt11 plays an essential role in neural crest migration. Inhibition of neural crest migration by blocking Wnt11 activity can be rescued by intracellular activation of the non-canonical Wnt pathway. When Wnt11 is expressed opposite its normal site of expression, neural crest migration is blocked. Finally, time-lapse analysis of cell movement and cell protrusion in neural crest cultured in vitro shows that the PCP or Wnt-Ca2+ pathway directs the formation of lamellipodia and filopodia in the neural crest cells that are required for their delamination and/or migration.

show abstract

Section: Discussionmentioning

confidence: 50%

“…Although no specific Wnt11 receptor has been identified, there is some evidence that suggests that PCP Wnt signalling involves Fz7 (Carreira-Barbosa et al, 2003;Djiane et al, 2000;Medina et al, 2000;Sumanas and Ekker, 2001;Winklbauer et al, 2001). We examined the distribution of the Fz7 receptor in neural crest cells.…”

Section: Resultsmentioning

confidence: 99%

Essential role of non-canonical Wnt signalling in neural crest migration

et al. 2005

View full text Add to dashboard Cite

show abstract

“…In the accompanying paper, we report a role for another Frizzled homolog, Xenopus frizzled-7, in regulating convergent extension as well as dorsoventral axis induction (Sumanas and Ekker, 2001). Zebrafish frizzled-2 is a distinct homolog from fz7 by expression pattern and sequence homology.…”

Section: Fig 1 Zebrafish Frizzled-2 Sequence Analysis and Expressiomentioning

confidence: 90%

Zebrafish frizzled‐2 morphant displays defects in body axis elongation

Sumanas

Kim

Hermanson

et al. 2001

Genesis

Self Cite

View full text Add to dashboard Cite

“…For instance, Xenopus Fz7 has been shown to mediate multiple intracellular responses. Fz7 affects convergent extension movements in a β-catenin/TCF-independent manner that involves Dishevelled, Syndecan 4 and Cdc42, but it is also required for dorsoventral mesoderm specification upstream of β-catenin/TCF (Medina et al, 2000;Munoz et al, 2006;Sumanas and Ekker, 2001;Sumanas et al, 2000). Similarly, many studies have demonstrated a role for Ror2 in the transmission of a Wnt5A signal that ultimately inhibits β-catenin/TCF signaling.…”

Section: Context-specific Functions For Cell Surface Receptorsmentioning

confidence: 99%

Towards an integrated view of Wnt signaling in development

2009

View full text Add to dashboard Cite

Wnt signaling is crucial for embryonic development in all animal species studied to date. The interaction between Wnt proteins and cell surface receptors can result in a variety of intracellular responses. A key remaining question is how these specific responses take shape in the context of a complex, multicellular organism. Recent studies suggest that we have to revise some of our most basic ideas about Wnt signal transduction. Rather than thinking about Wnt signaling in terms of distinct, linear, cellular signaling pathways, we propose a novel view that considers the integration of multiple, often simultaneous, inputs at the level of both Wnt-receptor binding and the downstream, intracellular response.

show abstract

Xenopus frizzled‐7 morphant displays defects in dorsoventral patterning and convergent extension movements during gastrulation

Cited by 41 publications

References 22 publications

Essential role of non-canonical Wnt signalling in neural crest migration

Essential role of non-canonical Wnt signalling in neural crest migration

Zebrafish frizzled‐2 morphant displays defects in body axis elongation

Towards an integrated view of Wnt signaling in development

Contact Info

Product

Resources

About