Yr58: A New Stripe Rust Resistance Gene and Its Interaction with Yr46 for Enhanced Resistance

Abstract

The quantitative trait loci QYr.sun-3BS and QYr.sun-4DL were identified in the W195/BTSS recombinant inbred line (RIL) population in a previous study. QYr.sun-3BS explained 34 to 59% phenotypic variation in stripe rust response. We evaluated parental genotypes at different growth stages and temperature regimes to detect the critical stage for expression of QYr.sun-3BS. W195 expressed low infection type (IT) ;1C at the fourth leaf stage, when incubated at 21 ± 2°C and the alternate parent BTSS was susceptible (IT 3). Monogenic segregation for stripe rust response was observed among the RIL population at the fourth leaf stage and the underlying locus was temporarily named YrW195. YrW195 corresponded to QYr.sun-3BS. Since no previously designated stripe rust resistance genes that expresses at and after the fourth leaf stage was mapped in this region, YrW195 was formally named Yr58. Genotyping with Yr46-linked markers indicated the presence of Yr46 in W195, which corresponded to QYr.sun-4DL. The RILs carrying Yr58 and Yr46 singly produced IT 23C and IT 3, respectively, and those carrying both genes produced IT ;1C indicating the enhancement of Yr58 expression by Yr46. The absence of Yr58-linked alleles of markers sun533 and sun476 in 74 of the 76 wheat cultivars demonstrated their usefulness for marker-assisted selection.

Genome-wide association mapping for resistance to leaf rust, stripe rust and tan spot in wheat reveals potential candidate genes

“…On chromosome 3B, two markers were significant but their positions could not be obtained. The catalogued rust resistance genes in this chromosome are Yr4 from common wheat (Bansal et al 2009 ) , Yr30/Sr2 that occurs in a high frequency in CIMMYT germplasm (Singh et al 2005 ) , Yr57 (Randhawa et al 2015 ) and Yr58 (Chhetri et al 2016 ). On chromosome 7DS, a marker at 3.7 cM was significant and Yr18/Lr34 is the catalogued gene in this chromosome which is present in a significant frequency in the CIMMYT germplasm.…”

Analysis of the primary sources of quantitative adult plant resistance to stripe rust in U.S. soft red winter wheat germplasm

“…The SSR marker cfb3530 was found to be closely linked to Yr4 at a distance of 2.9 ± 1.3 cM. The resistance gene Yr58 was identified in a doubled‐haploid population derived from resistant Indian spring wheat landrace W195 crossed with susceptible South Australian cultivar BTSS, in which it interacted with Yr46 on chromosome 4D to offer enhanced resistance (Chhetri, Bariana, Kandiah, & Bansal, 2016). Marker positions for Yr58 were defined using coordinates from the earlier contig‐level Wheat Chromosome Survey Sequence.…”

QTL Mapping of Wheat Stripe Rust Resistance in Relation to Epidermal Flavonoids Measured Using a Leaf‐Clip, Optical Sensor

“…The short arm of chromosome 3B harbors a number of Yr genes, including Yr4, Yr57, and Yr58. Waagan was reported by Chhetri et al (2016) to possess the Yr58 gene, and this would likely be the candidate gene, as the SNP marker IWA7918 is located close (?8.8 cM) to the barc75 marker identified for Yr58. Additional Waagan-derived resistance alleles were detected on chromosomes 2A, 4B, 5A, and 7D (Table 3, Fig.…”