<i>Zfy1</i> encodes a nuclear sequence‐specific DNA binding protein

doi:10.1016/0014-5793(95)00141-u

Cited by 13 publications

(2 citation statements)

References 33 publications

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“…The full-length proteins encoded by Zfy1 and Zfy2 are predicted to be zinc-finger transcription factors that bind the same DNA target sequences (Grants et al, 2010; Taylor-Harris et al, 1995); this suggests that Zfy1 may contribute to the impaired fertility of XY females. We therefore introduced into XO females either a single copy of a Y genomic BAC containing Zfy1 and Uba1y that had inserted on the X chromosome ( Zfy1 lo ) or 13-14 copies of the same BAC inserted on an autosome ( Zfy1 hi ) (Royo et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility

Vernet

Szot

Mahadevaiah³

et al. 2014

Development

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Outbred XYSry- female mice that lack Sry due to the 11 kb deletion Srydl1Rlb have very limited fertility. However, five lines of outbred XYd females with Y chromosome deletions YDel(Y)1Ct-YDel(Y)5Ct that deplete the Rbmy gene cluster and repress Sry transcription were found to be of good fertility. Here we tested our expectation that the difference in fertility between XO, XYd-1 and XYSry- females would be reflected in different degrees of oocyte depletion, but this was not the case. Transgenic addition of Yp genes to XO females implicated Zfy2 as being responsible for the deleterious Y chromosomal effect on fertility. Zfy2 transcript levels were reduced in ovaries of XYd-1 compared with XYSry- females in keeping with their differing fertility. In seeking the biological basis of the impaired fertility we found that XYSry-, XYd-1 and XO,Zfy2 females produce equivalent numbers of 2-cell embryos. However, in XYSry- and XO,Zfy2 females the majority of embryos arrested with 2-4 cells and almost no blastocysts were produced; by contrast, XYd-1 females produced substantially more blastocysts but fewer than XO controls. As previously documented for C57BL/6 inbred XY females, outbred XYSry- and XO,Zfy2 females showed frequent failure of the second meiotic division, although this did not prevent the first cleavage. Oocyte transcriptome analysis revealed major transcriptional changes resulting from the Zfy2 transgene addition. We conclude that Zfy2-induced transcriptional changes in oocytes are sufficient to explain the more severe fertility impairment of XY as compared with XO females.

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Section: Resultsmentioning

confidence: 99%

The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility

Vernet

Szot

Mahadevaiah³

et al. 2014

Development

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“…The transcription factors encoded by Zfy1 and Zfy2 , together with their X-linked homologue Zfx , are predicted to bind the same DNA sequence [ 26 – 28 ]. In most eutherian mammals Zfx and Zfy are widely expressed, and Zfx is exempt from X dosage compensation, suggesting a constraining dosage requirement in somatic tissues [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Mouse Y-Encoded Transcription Factor Zfy2 Is Essential for Sperm Head Remodelling and Sperm Tail Development

et al. 2016

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A previous study indicated that genetic information encoded on the mouse Y chromosome short arm (Yp) is required for efficient completion of the second meiotic division (that generates haploid round spermatids), restructuring of the sperm head, and development of the sperm tail. Using mouse models lacking a Y chromosome but with varying Yp gene complements provided by Yp chromosomal derivatives or transgenes, we recently identified the Y-encoded zinc finger transcription factors Zfy1 and Zfy2 as the Yp genes promoting the second meiotic division. Using the same mouse models we here show that Zfy2 (but not Zfy1) contributes to the restructuring of the sperm head and is required for the development of the sperm tail. The preferential involvement of Zfy2 is consistent with the presence of an additional strong spermatid-specific promotor that has been acquired by this gene. This is further supported by the fact that promotion of sperm morphogenesis is also seen in one of the two markedly Yp gene deficient models in which a Yp deletion has created a Zfy2/1 fusion gene that is driven by the strong Zfy2 spermatid-specific promotor, but encodes a protein almost identical to that encoded by Zfy1. Our results point to there being further genetic information on Yp that also has a role in restructuring the sperm head.

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ZFX and ZFY

Gazin

2002

Wiley Encyclopedia of Molecular Medicine

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Zfy1 encodes a nuclear sequence‐specific DNA binding protein

Cited by 13 publications

References 33 publications

The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility

The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility

Mouse Y-Encoded Transcription Factor Zfy2 Is Essential for Sperm Head Remodelling and Sperm Tail Development

ZFX and ZFY

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