<i>γ/δ</i> Cells in Fetal, Neonatal, and Adult RatLymphoid Organs

Kühnlein, P.; Vicente, Ángeles; Varas, Alberto; Hünig, Thomas; Zapata, A.

doi:10.1155/1995/73127

Cited by 22 publications

(7 citation statements)

References 11 publications

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“…Monoclonal Antibodies. The identity of hepatic infiltrating cells was determined using mouse anti-rat ␥␦ T-cell receptor [22][23][24] (Pharmingen, San Diego, CA) and mouse antirat T-cell antigen, W3/25 (CD4), antibodies. 25 The monoclonal antibody OX22, which reacts with CD45 isoforms containing exon C-encoded sequences (CD45RC), 26 also was utilized to identify the infiltrating cells.…”

Section: Pretreatment Of Recipientsmentioning

confidence: 99%

Cd45rc- γδ+ T–Cell Infiltration Is Associated With Immunologic Unresponsiveness Induced by Prior Donor–Specific Blood Transfusion in Rat Hepatic Allografts

et al. 2001

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Little is known regarding the role of ␥␦ ؉ T cells in organ transplantation. We previously reported that immunologic unresponsiveness is induced by prior donor-specific blood transfusion (DST) in rat hepatic allografts. We investigated the phenotype and distribution of ␥␦ ؉ T cells in the hepatic allograft, spleen, and peripheral blood of recipient rats with immunologic unresponsiveness induced by DST. ␥␦ ؉ T cells were enumerated in allograft livers and spleens by immunostaining and in blood by flow cytometric analysis. The phenotype of ␥␦ ؉ T cells was determined using CD45RC isoforms derived from alternative mRNA splicing. The great majority of mature human T lymphocytes express the ␣␤ T-cell receptor (TCR). A small population, which represents 0.5% to 16% of T lymphocytes in peripheral blood and lymphoid tissues, express the ␥␦ TCR. 1 Despite the fact that ␥␦ ϩ T cells are mature lymphocytes and have several phenotypic similarities to ␣␤ ϩ T cells, the biologic role of this cell population is still poorly understood. 2 ␥␦ ϩ T cells recognize class I and class II major histocompatibility complex (MHC) antigens. 3,4 However, non-MHC restricted cytotoxicity of ␥␦ ϩ T cells has been reported. 5 Thus, the preferential ligands for ␥␦ ϩ T cells have not yet been identified.Alloreactive ␥␦ ϩ T cells can trigger a rejection response, 6 and in fact, ␥␦ ϩ T cells are known to be involved in human renal allograft rejection 7 and in graft vascular disease. 8 However, ␥␦ ϩ T cells also may participate in cardiac allograft tolerance. 9,10 ␥␦ ϩ T cells from mice or rats selectively depleted of ␣␤ T cells seem to be unable to mount an alloreactive response, 11 but the role of ␥␦ ϩ T cells in organ transplantation is not clear.Leukocyte common antigen (LCA), CD45, has many isoforms derived from alternative mRNA splicing. 12 Six different CD45 isoforms have been identified from cDNA clones (usage of ABC, AB, BC, B, C, and O exons), and another is inferred (A exon alone) from hybridization studies. 13 Yang et al., 14 demonstrated functional maturation of recent thymic emigrants in the periphery, showing that the development of alloreactivity correlates with cyclic expression of CD45RC isoforms. LCA isoforms are associated with functional T-cell subsets including memory, activated, and alloreactive T cells. 15 In the rat, ␥␦ ϩ T-cell subsets are distinguished by membrane expression of the high (CD45RC ϩ ) and low (CD45RC Ϫ ) molecular weight isoforms of CD45. 16 We previously showed that persistent infiltration of CD45RC Ϫ CD4 ϩ T cells, Th2-like effector cells, was associated with immunologic unresponsiveness in rat hepatic allografts when animals were pretreated with donor-specific transfusion (DST) before transplantation. 17 However, little is known about the phenotype of ␥␦ ϩ T cells based on CD45RC expression in transplant immunology. The present study was undertaken to investigate the phenotype and distribution of ␥␦ ϩ T cells in rat hepatic allografts during acute rejection and their possible role in immunologic unrespons...

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Section: Pretreatment Of Recipientsmentioning

confidence: 99%

Cd45rc- γδ+ T–Cell Infiltration Is Associated With Immunologic Unresponsiveness Induced by Prior Donor–Specific Blood Transfusion in Rat Hepatic Allografts

et al. 2001

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“…On the other hand, one cannot exclude that the early stage of medulla organization may be controlled by signals mediated by TcR γδ (Farr et al 1990). In the rat thymus, such a mechanism may play a role in medulla organization between GD 16 and GD 17 (Kuhnlein et al 1995). Therefore, the organization of the medullary area, observed in this study between GD 15 and GD 16, might be linked rather with local effects of DCs on m-TECs, preceding the appearance of thymocytes at the appropriate stage of differentiation.…”

Section: Discussionmentioning

confidence: 68%

Homing of hemopoietic precursor cells to the fetal rat thymus: intercellular contact-controlled cell migration and development of the thymic microenvironment

Brelińska

Malińska

2005

Cell Tissue Res

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Colonization of rat thymic anlage by the first wave of hemopoietic precursor cells (HPc) was investigated by means of transmission electron microscopy and immunocytochemistry. HPc began migration into the thymic anlage between 13 and 13.5 gestation days (GD), terminated colonization at about GD 16, and migrated sequentially through the two compartments of the thymic anlage under the control of typical populations of stromal cells. First, HPc migrated through the external compartment of the perithymic mesenchyme, tightly interconnected with fibroblasts. The type of junctions between the cells indicated that the fibroblasts played a role in the control of HPc trafficking and in their entrance to the epithelial compartment. The second stage of colonization was initiated by the entrance of HPc to the epithelial compartment and their interaction with thymic epithelial cells (TECs). Based on morphological criteria, two populations of HPc were distinguished that colonized the anlage at various stages of its development. The predominant population with ultrastructural traits common to thymocytes "homed" into the epithelial type primordium. A small number of HPc, identified by protein S-100 expression and by Birbeck's granules as precursors of dendritic cells, colonized lymphoepithelial anlage in which subsets of cortical and medullary TECs could be distinguished. Thymocyte migration and their reciprocal interactions with cortical TECs differed from the trafficking of dendritic cells toward the medulla. The results demonstrated the influence of maturing thymocytes on the development of cortical epithelial cells and the dynamic organization of the medullary microenvironment with direct involvement of dendritic cells.

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“…Rat +ˇT cells bear strong similarities to mouse +ˇT cells with respect to V genes, ontogeny and tissue distribution ( [12][13][14][15][16], and this present study), but differ considerably from mouse +ˇT cells concerning coreceptor expression. A varying proportion of intraepithelial +ˇT cells in the gut and a consistently high proportion of +ˇT cells in lymphoid organs express the CD8 § g heterodimer [12,14].…”

Section: Introductionmentioning

confidence: 55%

Expression of functional CD8α β heterodimer on rat γ δ T cells does not correlate with the CDR3 length of the TCRδ chain predicted for MHC class I-restricted antigen recognition

Sträube¹,

Herrmann²

2000

Eur. J. Immunol.

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In accordance with their lack of MHC restriction, most mouse and human gamma delta T cells express neither the CD4 nor CD8(alpha beta) coreceptor. In striking contrast, up to 80% of splenic rat gamma delta T cells express the CD8alpha beta isoform of CD8, which for the alpha beta T cell subset serves as a marker for MHC class I-restricted cells. We compared CD8 on alpha beta and gamma delta T cells with regard to co-stimulatory function and correlation of CD8 expression with TCRDV usage and CDR3delta length. In both subsets, CD8 acted as a co-stimulatory molecule in vitro and was found to bind the kinase lck efficiently. No differences between the CDR3delta length spectra of CD8+ and CD8- gamma delta T cells or between unselected thymic and peripheral gamma delta T cells were found. As seen in man and mice, CDR3delta were rather long, a structural feature which can be expected to interfere with an alpha beta TCR-like mode of MHC class I binding. In summary, CD8 expressed by rat gamma delta T cells is a molecule with the potential to act as a coreceptor, but its expression gives no indication for antigen recognition analogous to that of MHC class I-restricted alpha beta T cells.

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γ/δ Cells in Fetal, Neonatal, and Adult RatLymphoid Organs

Cited by 22 publications

References 11 publications

Cd45rc- γδ+ T–Cell Infiltration Is Associated With Immunologic Unresponsiveness Induced by Prior Donor–Specific Blood Transfusion in Rat Hepatic Allografts

Cd45rc- γδ+ T–Cell Infiltration Is Associated With Immunologic Unresponsiveness Induced by Prior Donor–Specific Blood Transfusion in Rat Hepatic Allografts

Homing of hemopoietic precursor cells to the fetal rat thymus: intercellular contact-controlled cell migration and development of the thymic microenvironment

Expression of functional CD8α β heterodimer on rat γ δ T cells does not correlate with the CDR3 length of the TCRδ chain predicted for MHC class I-restricted antigen recognition

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