<i>Κ‐<scp>CN</scp></i> gene polymorphism in Nili‐ravi buffalo, Achai and Sahiwal cattle of Pakistan

Ghafoor, Abdul; Riaz, Muhammad Naeem; Zahur, Aamer Bin; Yousaf, Muhammad; Shah, Abdullah; Ishaq, Rafaqat; Suleman, Muhammad

doi:10.1111/1471-0307.12146

Cited by 6 publications

(5 citation statements)

References 19 publications

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“…Similar results of monomorphic "BB" were obtained in Nili-Ravi buffaloes using PCR-RFLP analysis with of (443 bp) fragment of PCR product of κ-CN with HindIII restriction enzyme as reported by Ghafoor et al (2015). Also, the restriction digestion analysis of (350 bp) PCR product of κ-CN with HinfI enzyme referred to the presence of only one genotype of "BB" in Nagpuri buffaloes of India as mentioned by Nair et al (2011).…”

Section: Allelic and Genotypic Frequencies Of Studied Genessupporting

confidence: 78%

“…These findings showed that the majority of studied individuals were heterozygous, i.e., these populations depended on outbreeding or mating with migrants. In disagreement with these results, Ghafoor et al (2015) noticed that there were no heterozygotes observed within Nili-ravi buffaloes due to all animals had homozygous genotype "BB".…”

Section: Heterozygosity and Inbreeding Coefficient (F Is ) Of κ-Cn Genementioning

confidence: 73%

“…PCR-RFLP results using AcuI enzyme disagreed with the majority of reviewed PCR-RFLP studies conducted by HindIII , HinfI or MaeII restriction enzymes which concluded that, all Egyptian buffaloes were monomorphic and genotyped as homozygous "BB" animals as reported by Othman (2005); El-Rafey and Darwish (2008); and Abdel Dayem et al (2009). Similar results were also noticed, in Pakistani buffalo (Riaz et al, 2008;Ghafoor et al, 2015), in Indian buffalo (Gangaraj et al, 2008;Shende et al, 2009;Nair et al, 2011;George et al, 2018), in Brazilian buffalo (Otaviano et al, 2005), in Chinese water buffalo (Ren et al, 2011) , in Iraqi buffalo (Jaayid et al, 2013), in Anatolian water buffalo breed (Cinar et al, 2016), in swamp and river Indonesian buffaloes (Margawati et al, 2016), in Cuban buffalo (Marrero et al, 2016), and in Iranian buffalo (Abbasi et al, 2009).…”

Section: Detection Of Polymorphism and Genetic Variants Of κ-Cn And β-Lgmentioning

confidence: 79%

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Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Al-Shawa¹,

El-Zarei²,

Ghazy³

et al. 2020

JAS

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With a view to detecting the genotypes of both κ-CN and β-LG genes in native populations of Egyptian buffalo using PCR-RFLP technique, 80 randomly, individuals were selected from five geographical locations of some Egyptian provinces. Also, to estimate the population genetic parameters such as, allelic and genotypic frequencies, heterozygosity, and inbreeding coefficient (F IS ) of these studied genes. For genotyping, 453 bp PCR product of κ-CN was digested with AcuI and HpyCH4IV (Isoschizomer for MaeII) restriction enzymes while the 247 bp PCR product of β-LG was digested with HaeIII restriction enzyme. PCR-RFLP results discovered polymorphism at the level of κ-CN gene in all studied Egyptian buffaloes with two distinct alleles "A" and "B". PCR-RFLP analysis for κ-CN gene using both restriction enzymes successfully detected that polymorphic status of the studied populations. We recommended using AcuI enzyme which was more capable for differentiating between homozygous (17%) and heterozygous (83%) individuals than HpyCH4IV enzyme which defined only 4% of homozygous individuals and the remaining was heterozygous (96%) individuals. Existence of heterozygosity excess in all studied populations referred to higher degree of genetic variability between individuals within these populations. On contrary, results of PCR-RFLP at the level of β-LG gene revealed a monomorphic pattern of Egyptian buffaloes and genotyped as "AA" animals which signified that PCR-RFLP assay with HaeIII enzyme for β-LG gene failed to discover any evidence of polymorphism in Egyptian buffalo under the circumstances of this study or all studied animals possess only one allele.

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Section: Allelic and Genotypic Frequencies Of Studied Genessupporting

confidence: 78%

Section: Heterozygosity and Inbreeding Coefficient (F Is ) Of κ-Cn Genementioning

confidence: 73%

Section: Detection Of Polymorphism and Genetic Variants Of κ-Cn And β-Lgmentioning

confidence: 79%

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Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Al-Shawa¹,

El-Zarei²,

Ghazy³

et al. 2020

JAS

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“…Similar to our results, Egyptian buffalo were reported to be BB monomorphic (Karima et al 2010). BB monomorphic buffaloes were also reported in Pakistan (Riaz et al 2008;Ghafoor et al 2014), Pandharpuri breed of India (Shende et al 2009), South Kanara and Surti breed of India (Gangaraj et al 2008) and Murrah breed of Brazil and their crossings (Otaviano et al 2008). All investigations, reporting BB monomorphic buffalo, were conducted by PCR-RFLP analyses using restriction enzymes such as HindIII and HinfI used for investigating polymorphism in cattle j-CN.…”

Section: Discussionmentioning

confidence: 99%

Genotyping of the kappa‐casein and beta‐lactoglobulin genes in Anatolian water buffalo by PCR‐RFLP

Çınar

Akyüz

Arslan

et al. 2015

Int J of Dairy Tech

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The objective of this study was to identify allele and genotype frequencies of the j-CN and b-LG genes in Anatolian water buffalo. A total of 126 water buffalos from Turkey were genotyped using the PCR-RFLP method. For gene j-CN, only B allele and BB genotype were observed. And for gene b-LG, two types of alleles (A and B) and three types of genotypes were observed. The genotype frequencies of AA, AB and BB of b-LG in Anatolian water buffalo were 0.254, 0.698 and 0.048, respectively. Surprisingly, the frequency of allele A was higher than that of allele B in contrast to world buffalo breeds.

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“…The kappa casein gene lies at chromosome 6 of bovine and at chromosome 4 of sheep and goat. Casein fraction of milk proteins significantly influences the composition and physic-chemical properties of milk (Grosclaud 1988) and milk production (Ghafoor et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

Margawati

Volkandari²,

Indriawati³

et al. 2017

Proceedings of International Seminar on Livestock Production and Veterinary Technology

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Casein (KCN) is a milk protein with four variants of alpha S1, alpha S2, beta and kappa which has different allel of each. Kappa Casein (CSN3) gene lies at exon 4 associating with fat and protein milk contens. Genetic variation of CSN3 gene influences quality and composition of milk. The aim of this research was to genotype the CSN3 gene of Bufalos from several places in Indonesia. Amount of 29 buffalo samples was used in this research, those consisted of 15 collected from Medan, 3 samples of Banyuwangi, 10 samples of Baluran and one sample of South-East Nusa Tenggara (NTT). A Polymerase Chain Reaction-Restiction Fragment Length Polymorphism (PCR-RFLP) method was used for CSN3 gene analysis. The CSN3 gene was amplified at 56°C of annealing with 30 cycles and resulted a right fragment of 379 bp. The fragments were digested with HindIII at 37°C during for four hours for genotyping analysis. The result showed that BB genotypes were found at the locus of CSN3 gene in all samples. Those BB genotypes merged the pattern of digested fragments with sizes of 255 and 154 bp. This finding suggests that genotyping using HindIII reveals monomorphics of BB types accociated with milk content of casein.

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Κ‐CN gene polymorphism in Nili‐ravi buffalo, Achai and Sahiwal cattle of Pakistan

Cited by 6 publications

References 19 publications

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Genotyping of the kappa‐casein and beta‐lactoglobulin genes in Anatolian water buffalo by PCR‐RFLP

Genotyping of Kappa-Casein Gene of Buffalo in Indonesian

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