Ibervillea sonorae (Cucurbitaceae) induces the glucose uptake in human adipocytes by activating a PI3K-independent pathway

Zapata-Bustos, Rocio; Alonso-Castro, Ángel Josabad; Gómez-Sánchez, Maricela; Salazar‐Olivo, Luis A.

doi:10.1016/j.jep.2014.01.041

Cited by 29 publications

(19 citation statements)

References 26 publications

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“…In a previous study, it was reported that coyote melon (Ibervillea sonorae) extract induces glucose uptake in adipocytes without insulin and that this effect is exerted by activation of a PI3 K-independent pathway [41]. This finding raises the possibility that the effect of 13-oxo-OTA is also exerted by a similar mechanism.…”

Section: Discussionmentioning

confidence: 96%

13‐Oxo‐9(Z),11(E),15(Z)‐octadecatrienoic Acid Activates Peroxisome Proliferator‐Activated Receptor γ in Adipocytes

Takahashi

Hara

Goto

et al. 2014

Lipids

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Peroxisome proliferator-activated receptor (PPAR)γ is expressed in adipose tissue and plays a key role in the regulation of adipogenesis. PPARγ activators are known to have potent antihyperglycemic activity and are used to treat insulin resistance associated with diabetes. Therefore, many natural and synthetic agonists of PPARγ are used in the treatment of glucose disorders. In the present study, we found that 13-oxo-9(Z),11(E),15(Z)-octadecatrienoic acid (13-oxo-OTA), a linolenic acid derivative, is present in the extract of tomato (Solanum lycopersicum), Mandarin orange (Citrus reticulata), and bitter gourd (Momordica charantia). We also found that 13-oxo-OTA activated PPARγ and induced the mRNA expression of PPARγ target genes in adipocytes, thereby promoting differentiation. Furthermore, 13-oxo-OTA induced secretion of adiponectin and stimulated glucose uptake in adipocytes. To our knowledge, this is the first study to report that 13-oxo-OTA induces adipogenesis through PPARγ activation and to present 13-oxo-OTA as a valuable food-derived compound that may be applied in the management of glucose metabolism disorders.

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Section: Discussionmentioning

confidence: 96%

13‐Oxo‐9(Z),11(E),15(Z)‐octadecatrienoic Acid Activates Peroxisome Proliferator‐Activated Receptor γ in Adipocytes

Takahashi

Hara

Goto

et al. 2014

Lipids

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“…Pharmacological studies show that plant root extracts display hypoglycemic and antiinflammatory (Alarcon-Aguilar et al, 2005;Jard on-Delgado et al, 2014;Rivera-Ramírez et al, 2011;Zapata-Bustos et al, 2014), antioxidant (Estrada-Zuñiga et al, 2012), antimicrobial (Robles-Zepeda et al, 2011), and antifungal activities (Ruiz-Bustos et al, 2009). A recent study conducted on human preadipocyte cells showed that aqueous root extracts from I. sonorae stimulate glucose uptake by a PI3K independent pathway (Zapata-Bustos et al, 2014). This evidence supports the antidiabetic properties attributed to I. sonorae roots in traditional medicine.…”

mentioning

confidence: 82%

“…It produces secondary metabolites such as alkaloids, tannins, saponins (Alarcon-Aguilar et al, 2005), flavonoids, phenols, (Zapata-Bustos et al, 2014) and cucurbitacins (Achenbach et al, 1993;Jard on-Delgado et al, 2014). Pharmacological studies show that plant root extracts display hypoglycemic and antiinflammatory (Alarcon-Aguilar et al, 2005;Jard on-Delgado et al, 2014;Rivera-Ramírez et al, 2011;Zapata-Bustos et al, 2014), antioxidant (Estrada-Zuñiga et al, 2012), antimicrobial (Robles-Zepeda et al, 2011), and antifungal activities (Ruiz-Bustos et al, 2009). A recent study conducted on human preadipocyte cells showed that aqueous root extracts from I. sonorae stimulate glucose uptake by a PI3K independent pathway (Zapata-Bustos et al, 2014).…”

mentioning

confidence: 99%

Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

Arciniega-Carreón

Oliver-Salvador

Ramírez-Sotelo

et al. 2017

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Ibervillea sonorae is a medicinal plant mainly used to treat diabetes, ulcers, and other metabolic disorders. A regeneration protocol using internode segments containing axillary buds grown on Gamborg medium (B5) supplemented with 0.5 mg·L−1 α-naphthalene-acetic acid (NAA), 0.5 mg·L−1 N6-benzyladenine (BA), and 1.0 mg·L−1 indole-3-acetic acid (IAA) successfully regenerated shoots in I. sonorae explants. The induction of organogenic calli attained 100% efficiency. The highest percent shoot production was 87.5% with a mean of 9.17 shoots per explant on day 15, and the maximum length of 5.8 cm was observed on day 21. Regenerated shoots induced roots in B5 medium supplemented with 0.5–3.0 mg·L−1 indole-3-butyric acid (IBA). The maximum rooting frequency observed in the medium containing 2.0 mg·L−1 IBA was 83.3% which promoted long, thick roots on day 21. The plantlets with emerging roots grown at the culture facility attained 50% survival after acclimatization for 30 d. The account describes a simple and efficient protocol for in vitro plant regeneration, and this micropropagation procedure offers an alternative for preservation of this medicinal plant.

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“…Ibervillea sonorae has been used mainly to treat diabetes due to its hypoglycemic activity [41]- [43]. RuizBustos et al [44] evaluated the antimicrobial activity of I. sonorae against fungi as well as Gram-positive and Gram-negative bacteria.…”

Section: Ibervillea Sonoraementioning

confidence: 99%

Cytotoxic Effect of Methanol Extracts and Partitions of Two Mexican Desert Plants against the Murine Lymphoma L5178Y-R

Quintanilla-Licea¹,

Gómez-Flores²,

Samaniego-Escamilla³

et al. 2016

AJPS

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Pachycereus marginatus (DC.) Britton & Rose and Ibervillea sonorae (S. Watson) Greene have been used in the Mexican traditional medicine for the treatment of various diseases, including cancer. The present study aims to investigate the cytotoxic activity of these plants against a murine lymphoma. Soxhlet extraction of dried and powdered plant material was performed with methanol. Also, a further partitioning of these methanolic extracts with hexane and ethyl acetate was achieved. The in vitro cytotoxic activity against the murine lymphoma L5178Y-R cell line was assessed via the colorimetric MTT assay. The methanol extract from P. marginatus exhibited high cytotoxic activity (up to 94%) at concentrations ranging from 3.9 to 500 µg/mL; however, hexane and ethyl acetate partitions from this methanolic extract showed lower but significant (p < 0.05) concentration-dependent cytotoxicity (hexane partition up to 94% at 500 µg/mL; ethyl acetate partition up to 94% at 65.5 µg/mL). The methanolic extract and partitions derived from I. sonorae also showed significant (p < 0.05) and concentration-dependent cytotoxicity against L5178Y-R cells at concentrations ranging from 7.81 to 500 µg/mL (methanolic extract up to 63% at 500 µg/mL; hexane partition up to 76% at 250 µg/mL; ethyl acetate partition up to 73% at 500 µg/mL). These results demonstrate that the methanol extracts and partitions from P. marginatus and I. sonorae possess significant cytotoxic activity against the murine lymphoma L5178Y-R and validate the ethnobotanical use of these plants for the treatment of diseases consistent with cancer symptomatology. Previous scientific reports describe the isolation of isoquinoline alkaloids of P. margi-* Corresponding author. R. Quintanilla-Licea et al. 1522natus as well as cucurbitacins from I. sonorae, phytochemicals that could be responsible for their observed cytotoxic activity in this research. The direct extraction with methanol of medicinal plants allows extracting of both high and low-polarity compounds, contrary to the simple extraction with water that only allows obtaining compounds of high polarity. The subsequent partition of the methanol extract with a solvent of low polarity (hexane) and another of medium polarity (ethyl acetate) allows making a preliminary fractionation of the bioactive molecules present in the plant that will facilitate the bioguided chromatographic isolation of the pure compounds responsible for the biological activity of the plant.

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Ibervillea sonorae (Cucurbitaceae) induces the glucose uptake in human adipocytes by activating a PI3K-independent pathway

Cited by 29 publications

References 26 publications

13‐Oxo‐9(Z),11(E),15(Z)‐octadecatrienoic Acid Activates Peroxisome Proliferator‐Activated Receptor γ in Adipocytes

13‐Oxo‐9(Z),11(E),15(Z)‐octadecatrienoic Acid Activates Peroxisome Proliferator‐Activated Receptor γ in Adipocytes

Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

Cytotoxic Effect of Methanol Extracts and Partitions of Two Mexican Desert Plants against the Murine Lymphoma L5178Y-R

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