ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming

Ségura, Elodie; Nicco, Carole; Lombard, Bérangère; Véron, Philippe; Raposo, Graça; Batteux, Frédéric; Amigorena, Sébastian; Théry, Clotilde

doi:10.1182/blood-2005-01-0220

Cited by 523 publications

(526 citation statements)

References 40 publications

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“…To test the potential for exosomes to be used as cell-free vaccines against cancers, DC-derived exosomes have undergone phase I trial and are currently in phase II trial [12,103–105]. In addition to MHC-bound antigens expressed on the exosomal surface, mature DC-derived exosomes also show surface expression of ICAM-1, which is important for the induction of immune responses, such as T-cell activation [106], and the NKG2D ligand, which is a TNF superfamily ligand that binds directly to NK cells to induce their activation/proliferation and cause an anti-tumour immune response [107,108]. …”

Section: Therapeutic Applications Of Surface-modified Evsmentioning

confidence: 99%

Extracellular vesicles as a platform for membrane‐associated therapeutic protein delivery

Yang

Hong

Cho

et al. 2018

J of Extracellular Vesicle

190

148

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Membrane proteins are of great research interest, particularly because they are rich in targets for therapeutic application. The suitability of various membrane proteins as targets for therapeutic formulations, such as drugs or antibodies, has been studied in preclinical and clinical studies. For therapeutic application, however, a protein must be expressed and purified in as close to its native conformation as possible. This has proven difficult for membrane proteins, as their native conformation requires the association with an appropriate cellular membrane. One solution to this problem is to use extracellular vesicles as a display platform. Exosomes and microvesicles are membranous extracellular vesicles that are released from most cells. Their membranes may provide a favourable microenvironment for membrane proteins to take on their proper conformation, activity, and membrane distribution; moreover, membrane proteins can cluster into microdomains on the surface of extracellular vesicles following their biogenesis. In this review, we survey the state-of-the-art of extracellular vesicle (exosome and small-sized microvesicle)-based therapeutics, evaluate the current biological understanding of these formulations, and forecast the technical advances that will be needed to continue driving the development of membrane protein therapeutics.

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Section: Therapeutic Applications Of Surface-modified Evsmentioning

confidence: 99%

Extracellular vesicles as a platform for membrane‐associated therapeutic protein delivery

Yang

Hong

Cho

et al. 2018

J of Extracellular Vesicle

190

148

View full text Add to dashboard Cite

show abstract

“…These exosomes are enriched in membrane proteins involved in antigen presentation, including MHC Class I and II molecules, MHC Class I-like molecule (CD1), co-stimulatory molecules (CD80 and CD86), adhesion molecule (ICAM-1, MFG-E8). [64][65][66][67] Proteomic analysis of DEXs also identified new exosomal proteins, which were mainly cytoskeleton-related molecules (cofilin and profilin I) and membrane transport and signaling factors (annexins, rab 7 and 11, rap1B, and syntenin), suggesting a role of exosomes in transmembrane transport. 64 Furthermore, HSP73 was another exosomal content presented in endocytic compartments of DCs, which could elicit antitumor responses through T cell-dependent or -independent way.…”

Section: Exosome-mediated Activation Of Immune Response Against Tumorsmentioning

confidence: 99%

The exosomes in tumor immunity

2015

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Exosomes are a kind of nanometric membrane vesicles and can be released by almost all kinds of cells, including cancer cells. As the important mediators in intercellular communications, exosomes mediate exchange of protein and genetic material derived from parental cells. Emerging evidences show that exosomes secreted by either host cells or cancer cells are involved in tumor initiation, growth, invasion and metastasis. Moreover, communications between immune cells and cancer cells via exosomes play dual roles in modulating tumor immunity. In this review, we focus on exosome-mediated immunosuppression via inhibition of antitumor responses elicited by immune cells (DCs, NK cells, CD4 C and CD8 C T cells, etc.) and induction of immunosuppressive or regulatory cell populations (MDSCs, Tregs and Bregs). Transfer of cytokines, microRNAs (miRNAs) and functional mRNAs by tumor-derived exosomes (TEXs) is crucial in the immune escape. Furthermore, exosomes secreted from several kinds of immune cells (DCs, CD4 C and CD8 C Tregs) also participate in immunosuppression. On the other hand, we summarize the current application of DCderived and modified tumor-derived exosomes as tumor vaccines. The potential challenges about exosome-based vaccines for clinical application are also discussed.

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“…It has been shown that mast cell derived exosomes are able to induce maturation of DCs by upregulation of MHCII, CD40, CD80 and CD86 17 or exosomes from antigen presenting cells are involved in T-cell stimulation both in vitro 18 and in vivo. 19 However, it is not known how much of this communication pathway is dependent on esRNA? It may be that at least two types of exosomes exist, (1) the immunological active exosomes that are involved in antigen presenting and co-stimulation and (2) the type two exosomes that contain RNA and mediating genetic communication between cells.…”

Section: The Putative Roles Of Exosomal Shuttle Rnamentioning

confidence: 99%

Cell to Cell Signalling via Exosomes Through esRNA

Lötvall¹,

Valadi²

2007

Cell Adhesion & Migration

247

194

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Exosomes are small vesicles of endosomal origin that can be released by many different cells to the microenvironment. Exosomes have been shown to participate in the immune system, by mediating antigen presentation. We have recently shown the presence of both mRNA and microRNA in exosomes, specifically in exosomes derived from mast cells. This RNA can be transferred between one mast cell to another, most likely through fusion of the exosome to the recipient cell membrane. The delivered RNA is functional, as the mRNA can lead to translation of new proteins in a recipient cell. The RNA shuttled between cells via exosomes is called esRNA. We propose that several types of exosomes may exist, and that an additional function of exosomes is to communicate to neighbouring cells through delivery of RNA-signals.

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ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming

Cited by 523 publications

References 40 publications

Extracellular vesicles as a platform for membrane‐associated therapeutic protein delivery

Extracellular vesicles as a platform for membrane‐associated therapeutic protein delivery

The exosomes in tumor immunity

Cell to Cell Signalling via Exosomes Through esRNA

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