Icariin prevents ovariectomy-induced bone loss and lowers marrow adipogenesis

Li, Guanwu; Zheng, Xu; Chang, Shixin; Ni, Hua; Wang, Xiaoyan; Qin, Lin-Di

doi:10.1097/gme.0000000000000201

Cited by 41 publications

(31 citation statements)

References 51 publications

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“…20) For phytoestrogens, icariin prevented ovariectomy-induced bone loss and lowered marrow adipogenesis, comparabley with estrogen. 19) Also, puerarin increased adipocyte differentiation, 23) Our data was different from all the above. We found that the OVX-induced adipocyte-increase and adiponectinincrease in supernatant was suppressed significantly by intake of puerarin.…”

Section: Fig 2 Microtomographic Histomorphometry Of the Proximal Tibiacontrasting

confidence: 49%

“…19) A reciprocal association between osteoblasts and adipocytes deriving from bone marrow stromal cells prompted us to explore the effect of puerarin on adipogenesis at the same time. Unfortunately, our data indicated that the reciprocal changes in bone and adiposity were not directly coupled with one another.…”

Section: Fig 2 Microtomographic Histomorphometry Of the Proximal Tibiamentioning

confidence: 99%

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Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats

Liu

Jia

2014

Biological & Pharmaceutical Bulletin

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We investigated the effect of puerarin on bone mass and marrow adiposity in ovariectomy (OVX)-induced osteoporosis. The rats were divided into four groups: control; OVX; OVX estradiol (OVX-E); and OVX puerarin treatment (OVX-GE). In vivo, bone mineral density (BMD) and histomorphometry were measured under microCT. The mechanical properties of tibia were obtained in 3-point bending test. Plasma osteocalcin and adiponectin were determined using enzyme-linked immunosorbent assay (ELISA). Alkaline phosphatase (ALP) were measured using biochemical methods. In vitro, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Oil Red O staining were used to compare osteoblast proliferation and adipocyte differentiation, respectively. Osteocalcin and adiponectin in culture supernatants were determined using ELISA. The results showed that puerarin significantly enhanced bone volume density and trabecular number compared with OVX and OVX-E groups (p<0.05, p<0.05, respectively). Puerarin increased energy to ultimate load, plasma osteocalcin and ALP (p<0.01). However, BMD in OVX-GE group was less than that in control (p<0.01) and OVX-E groups (p<0.05). The culture supernatants from OVX-GE group showed increased osteocalcin compared with those from OVX ( p<0.01) and OVX-E groups ( p<0.05). Puerarin lowered adiponectin in culture supernatant compared with supernatant from OVX group and inhibited the increase in adipocytes caused by OVX (p<0.01). However, the amount of lipids did not differ between OVX-GE and OVX groups. These findings suggest that puerarin likely enhances bone formation by stimulating the proliferation and differentiation of osteoblasts while slightly inhibiting the adipotic differentiation.Key words puerarin; bone mass; bone formation; adiposity; osteoporosis Puerariae radix, the root of Pueraria lobata (WILD.) OTRWI, a wild creeper leguminous plant, is one of the earliest and most important crude herbs used in Chinese medicine for various medicinal purposes. For the near decade, it has been reported using osteoporosis animal model that total isoflavones of puerariae isoflavone could protect osteoporosis induced by ovariectomized rats 1,2) and could inhibit the secondary osteoporosis induced by dexamethasone in rats.3) As a kind of phytoestrogen, puerarin is regarded as one of isoflavone extract which exerting preventive effect on bone loss in ovariectomized rats.4) However, all the related animal experiment used total isoflavones of Pueraria lobata (WILD.) OTRWI. Their results were the comprehensive effects of daidzein, genistein and puerarin. We couldn't judge the independent effect of puerarin on bone gain.Puerarin is an active component extracted from the Chinese traditional medicine Pueraria lobata (WILD.) OTRWI. Structurely, puerarin is similar to estrogen. It has three -OH, where they combine with estradiol receptors and then exhibit estrogenic action. Therefore, it's believed to exhibit beneficial effect on bone tissue by mimicking the biological responses of estrogens.5,6) Previously, we...

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Section: Fig 2 Microtomographic Histomorphometry Of the Proximal Tibiacontrasting

confidence: 49%

Section: Fig 2 Microtomographic Histomorphometry Of the Proximal Tibiamentioning

confidence: 99%

Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats

Liu

Jia

2014

Biological & Pharmaceutical Bulletin

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“…Furthermore, biomechanics allow us to identify the biological effects of bone under force or stress [23]. Previous studies have proved that although low bone mass in osteoporotic bones is a major risk factor for the occurrence of a fracture, the presence of adequate trabecular microstructure can preserve the biomechanical properties of the bone [25,26].…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Amphimas pterocarpoides Plant Extract on Bone Mineral Density and Strength in Estrogen Deficient Ovariectomized Wistar Rats

Patsaki¹,

Tchoumtchoua²,

Passali³

et al. 2016

Med Aromat Plants

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“…It has been reported that ICA can improve bone mineral density (BMD) and bone strength in ovariectomized rats [20]. Moreover, Li et al reported that ICA prevented ovariectomyinduced bone loss and lowered marrow adipogenesis [21]. Meanwhile our previous study demonstrated that ICA might have a beneficial effect on the BMSCs of patients with steroidassociated osteonecrosis by demethylation of the adenosine triphosphate-binding cassette (ABC) B1-promoter [14].…”

Section: Introductionmentioning

confidence: 99%

Icariin Protects against Glucocorticoid-Induced Osteonecrosis of the Femoral Head in Rats

Huang

Cheng

Cao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Glucocorticoid (GC)-related osteonecrosis of the femoral head (ONFH) is a common complication following administration of steroids to treat many diseases. Our previous study demonstrated that icariin (ICA) might have a beneficial effect on the bone marrow mesenchymal stem cells (BMSCs) of patients with steroid-associated osteonecrosis. In this study, we investigated the underlying mechanisms of ICA associated with the potential enhancement of osteogenesis and anti-adipogenesis in GC-related ONFH. Methods: In vitro cell proliferation was evaluated by CCK-8 assay. Alizarin red S and alkaline phosphatase (ALP) activity were used to measure osteogenic differentiation, while adipogenic differentiation was revealed by oil red O staining and TG content assay. The expression level of osteogenesis-associated genes and PPARγ was evaluated by RT-qPCR, western blotting and immunofluorescence. A total of 30 female SD rats were randomly separated into three groups: a control group, a methylprednisolone (MPS) group and a MPS + ICA group. Serum ALP and TG (triglyceride), micro-CT scanning, histological and immunohistochemical analyses were performed in the animal model. Results: In the in vitro study, ICA promoted proliferation, improved osteogenic differentiation and suppressed adipogenic differentiation of BMSCs treated with MPS. The group treated with MPS and 10^-6 M ICA expressed higher levels of Runx2, ALP, bone morphogenetic protein (BMP) 2, and OC and lower expression of PPARγ than the MPS group. In the in vivo study, ICA prevented bone loss in a rat model of GC-related ONFH as shown by micro-CT scanning, histological and immunohistochemical analyses. Conclusions: ICA is an effective compound for promoting bone repair and preventing or delaying the progression of GC-associated ONFH in rats. This effect can be explained by its ability to improve the balance between adipogenesis and osteogenesis, indicating that ICA is an effective candidate for management of GC-associated ONFH.

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Icariin prevents ovariectomy-induced bone loss and lowers marrow adipogenesis

Abstract: Early icariin treatment restores marrow adiposity in the estrogen-deficient rat model.

Cited by 41 publications

References 51 publications

Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats

Puerarin Enhances Bone Mass by Promoting Osteoblastogenesis and Slightly Lowering Bone Marrow Adiposity in Ovariectomized Rats

The Protective Effect of Amphimas pterocarpoides Plant Extract on Bone Mineral Density and Strength in Estrogen Deficient Ovariectomized Wistar Rats

Icariin Protects against Glucocorticoid-Induced Osteonecrosis of the Femoral Head in Rats

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