Icariin regulates PRMT/ADMA/DDAH pathway to improve endothelial function

Xiao, Hong-Bo; Liu, Zi-Kui; Lu, Xiangyang; Deng, Chun-Na; Luo, Zhigang

doi:10.1016/j.pharep.2015.04.017

Cited by 19 publications

(13 citation statements)

References 37 publications

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“…The TD and icariin group were fed same diet as control group but with the addition of tetramethylthiuram disulfide (thiram) 50 mg/kg of feed from days 3 to 7 posthatch to induce TD. After induction of TD on day 8, the icariin group was separated and fed with standard normal diet with the addition of 10 mg/kg/day icariin ( Xiao et al, 2015 ) through drinking water until the end of experiment, while TD group was given standard normal diet without adding thiram, just like control group.…”

Section: Methodsmentioning

confidence: 99%

Icariin Ameliorate Thiram-Induced Tibial Dyschondroplasia via Regulation of WNT4 and VEGF Expression in Broiler Chickens

Zhang

Mehmood

et al. 2018

Front. Pharmacol.

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Tibial dyschondroplasia (TD) is main bone problem in fast growing poultry birds that effect proximal growth plate (GP) of tibia bone. TD is broadly defined as non-vascularized and non-mineralized, and enlarged GP with tibia bone deformation and lameness. Icariin (Epimedium sagittatum) is a traditional Chinese medicine, which is commonly practiced in the treatment of various bone diseases. Recently, many researcher reports about the beneficial effects of icariin in relation to various types of bone conditions but no report is available about promoting effect of icariin against TD. Therefore, current study was conducted to explore the ameliorating effect of icariin in thiram-induced TD chickens. A total of 180 broiler chicks were equally distributed in three groups; control, TD induced by thiram (50 mg/kg), and icariin group (treated with icariin @10 mg/kg). All groups were administered with normal standard diet ad libitum regularly until the end of experiment. The wingless-type member 4 (WNT4) and vascular endothelial growth factor (VEGF) genes and proteins expression were analyzed by quantitative real-time polymerase chain reaction and western blot analysis respectively. Tibial bone parameters, physiological changes in serum, antioxidant enzymes, and chicken growth performance were determined to assess advantage and protective effect of the medicine in broiler chicken. The expression of WNT4 was decreased while VEGF increased significantly (P < 0.05) in TD affected chicks. TD enhanced the GP, lameness, and irregular chondrocytes, while reduced the liver function, antioxidant enzymes in liver, and performance of chickens. Icariin treatment up-regulated WNT4 and down-regulated VEGF gene and protein expressions significantly (P < 0.05), restored the GP width, increased growth performance, corrected liver functions and antioxidant enzymes levels in liver, and mitigated the lameness in broiler chickens. In conclusion, icariin administration recovered GP size, normalized performance and prevented lameness significantly. Therefore, icariin treatments are encouraged to reduce the incidence of TD in broiler chickens.

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Section: Methodsmentioning

confidence: 99%

Icariin Ameliorate Thiram-Induced Tibial Dyschondroplasia via Regulation of WNT4 and VEGF Expression in Broiler Chickens

Zhang

Mehmood

et al. 2018

Front. Pharmacol.

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“…Previous studies have suggested that Icariin attenuated the development of atherosclerosis in several animal models, including ApoE -/- mice ( Xiao et al, 2015 , 2017 ; Wang et al, 2016 ), rats ( Hu et al, 2015b ), and rabbits ( Zhang et al, 2013 ). The atheroprotective mechanisms of Icariin are exerted through anti-oxidative, anti-inflammatory, anti-thrombotic, and lipid-modulatory effects.…”

Section: Anti-atherosclerotic Effects Of Icariinmentioning

confidence: 99%

“…Furthermore, Icariin also increase eNOS activity by blocking eNOS uncoupling through decreasing the level of ROS and asymmetric dimethylarginine (ADMA) by increasing the level of dimethylarginine dimethylaminohydrolase (DDAH) in ApoE -/- mice. The resulted effect is increasing endothelium-dependent vasorelaxation ( Xiao et al, 2015 ). Recent evidence has also suggested that Icariin reduces oxLDL-induced monocyte adhesion to endothelial cells, the initiating event in atherosclerosis.…”

Section: Anti-atherosclerotic Effects Of Icariinmentioning

confidence: 99%

Icariin, an Anti-atherosclerotic Drug from Chinese Medicinal Herb Horny Goat Weed

Fang

Zhang

2017

Front. Pharmacol.

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Icariin is a major bioactive pharmaceutical constituent isolated from Chinese medicine Horny Goat Weed (Ying Yang Huo) with potent cardiovascular protective functions. Emerging evidence in the past decade has shown that Icariin possesses multiple atheroprotective functions, through multiple mechanisms, including attenuating DNA damage, correcting endothelial dysfunction, inhibiting the proliferation and migration of smooth muscle cells, repressing macrophage-derived foam cell formation and inflammatory responses, as well as preventing platelet activation. All of these protective effects, combined with its lipid-modulatory effects, contribute to the broad atheroprotective effects of Icariin. In this review, we will summarize the anti-atherosclerotic properties of Icariin and highlight future perspectives in developing Icariin as a promising anti-atherosclerotic drug.

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“…For one thing, DDAH activity is negatively regulated by lysophosphatidylcholine, homocysteine, and oxidized low‐density lipoprotein. For another thing, DDAH is positively modulated by antioxidant such as icariin, all‐trans‐Retinoic acid, pyrrolidine dithiocarbamate, and xanthone . Additional investigations reports that elevated productions of ROS reduce the level of bioactive NO .…”

Section: Discussionmentioning

confidence: 99%

Procyanidin B2 from lotus seedpod regulate NO/ADMA/DDAH pathway to treat insomnia in rats

Wang

Liang

et al. 2019

Fundamemntal Clinical Pharma

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Recent studies show that nitric oxide/asymmetric dimethylarginine/dimethylarginine dimethylaminohydrolase (NO/ADMA/DDAH) pathway may contribute to the development of sleep disorder. The objective of this study was to explore the inhibitory effect of procyanidin B2 from lotus seedpod (LSPC), a naturally occurring catechin compound, on insomnia and the mechanisms involved. The experiments were performed in brain from Sprague‐Dawley rat control and insomniac rats treated or not with LSPC (15, 30, and 45 mg/kg, intragastrically) for 7 days. LSPC treatment reduced walking time and forelimb lifting‐up frequency, cerebral levels of noradrenaline, glutamic acid, ADMA, sleep latency, and 8‐isoprostane; increased sleep duration, cerebral concentrations of 5‐hydroxytryptamine, γ‐aminobutyric acid, and NO concomitantly with upregulated cerebral expression of DDAH 1, DDAH2, and neuronal NO synthases in insomniac rats. The present results suggest that LSPC may regulate NO/ADMA/DDAH pathway by inhibiting oxidative stress to treat insomnia in rats when sleep evaluation was achieved on the basis of behavioral criteria.

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Icariin regulates PRMT/ADMA/DDAH pathway to improve endothelial function

Abstract: The present results suggest that icariin regulates PRMT/ADMA/DDAH pathway to improve endothelial function.

Cited by 19 publications

References 37 publications

Icariin Ameliorate Thiram-Induced Tibial Dyschondroplasia via Regulation of WNT4 and VEGF Expression in Broiler Chickens

Icariin Ameliorate Thiram-Induced Tibial Dyschondroplasia via Regulation of WNT4 and VEGF Expression in Broiler Chickens

Icariin, an Anti-atherosclerotic Drug from Chinese Medicinal Herb Horny Goat Weed

Procyanidin B2 from lotus seedpod regulate NO/ADMA/DDAH pathway to treat insomnia in rats

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