Icariin triggers osteogenic differentiation of bone marrow stem cells by up-regulating miR-335–5p

Bian, Si-Shan; Kong, Ping; Chen, Yun-Gang

doi:10.1016/j.yexcr.2022.113085

Cited by 15 publications

(12 citation statements)

References 33 publications

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“…Traditional Chinese medicine has played an important role in the treatment of OP. Multiple studies have confirmed the effect of different herbal medicines on the osteogenic differentiation of BM-MSCs (Chen et al, 2019;Fei et al, 2022;Huang et al, 2020;Song et al, 2022;Teng et al, 2022). However, the mechanism by which AR alleviates OP by promoting osteogenesis differentiation has not been adequately studied to date.…”

Section: Discussionmentioning

confidence: 99%

Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation

Tian,

Li,

Wang

et al. 2023

Chem Biol Drug Des

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The present study used network pharmacology and molecular docking to predict the active ingredients and mechanisms of action of Astragalus radix (AR) to promote osteogenic differentiation of bone marrow mesenchymal stem cells (BM‐MSCs), and cell experiments were conducted for verification. First, network pharmacology was used to predict the effective components, targets, and mechanisms of action of AR to promote osteogenic differentiation. The effective components and corresponding target proteins of AR, and the target proteins of osteogenic differentiation were collected through the database. The intersection targets of the two were used for the construction and analysis of a protein–protein interaction (PPI) network. Gene Oncology (GO) and Kyoto Encyclopedia of Genes, and Genomes (KEGG) enrichment analyses were conducted. Next, molecular docking technology was carried out to verify the interaction between the active ingredient and the target protein, and to select the appropriate effective active ingredient. Finally, the results of network pharmacology analysis were verified by in vitro experiments. A total of 95 potential targets were retrieved by searching the intersection of AR and osteogenic differentiation targets. PPI network analysis indicated that RAC‐α‐serine‐threonine‐protein kinase (Akt1) was considered to be the most reliable target for AR to regulate osteogenic differentiation. GO enrichment analysis included 21 biological processes, 21 cellular components and 100 molecular functions. KEGG enrichment analysis indicated that the class I phosphatidylinositol‐3 kinase (PI3K)‐serine‐threonine kinase (Akt) signaling pathway may play an important role in promoting osteogenic differentiation. The results of molecular docking showed that quercetin's performance was improved compared with that of kaempferol. In vitro experiments showed that quercetin promoted the expression of osteogenic marker proteins (including collagen I, Runt‐related transcription factor 2 and osteopontin) in BMSCs and activated the PI3K/Akt signaling pathway. AR acted on Akt1 targets through its main active component quercetin, and promoted the osteogenic differentiation of BM‐MSCs by activating the PI3K/Akt signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation

Tian,

Li,

Wang

et al. 2023

Chem Biol Drug Des

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“…One study confirmed that icariin has robust bone-promoting activity, making it an excellent candidate for postmenopausal OP therapy ( Wang et al, 2017 ). Icariin modulates bone differentiation by elevating miR-335-5p levels ( Teng et al, 2022 ). Furthermore, Icariin restores bone loss and suppresses OP development and progression by reducing miR-34c levels ( Liu J et al, 2016 ).…”

Section: Progress In Op Therapy Via Regulation Of Epigenetic Inherita...mentioning

confidence: 99%

Therapeutic approach of natural products that treat osteoporosis by targeting epigenetic modulation

Zhang

Liu

et al. 2023

Front. Genet.

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Osteoporosis (OP) is a metabolic disease that affects bone, resulting in a progressive decrease in bone mass, quality, and micro-architectural degeneration. Natural products have become popular for managing OP in recent years due to their minimal adverse side effects and suitability for prolonged use compared to chemically synthesized products. These natural products are known to modulate multiple OP-related gene expressions, making epigenetics an important tool for optimal therapeutic development. In this study, we investigated the role of epigenetics in OP and reviewed existing research on using natural products for OP management. Our analysis identified around twenty natural products involved in epigenetics-based OP modulation, and we discussed potential mechanisms. These findings highlight the clinical significance of natural products and their potential as novel anti-OP therapeutics.

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“…104,105 To our excitement, besides, microRNA also plays a crucial role in mediating the promotion of ICA to osteoblast differentiation. 106 An in vitro investigation shown that ICA had a potent influence on osteoblast development in MC3T3-E1 cells via the miR-153/ Runx2 pathway. 107 Even though MC3T3-E1 cells are not MSCs, this result gave rise to the possibility that ICA might improve MSCs' ability to differentiate into osteoblasts through a microRNA-mediated mechanism.…”

Section: Icariin Regulates Migration Proliferation and Differentiatio...mentioning

confidence: 99%

Icariin: A Potential Alternative Against Osteoporosis

Long

Wang

Lijun

et al. 2022

Natural Product Communications

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Osteoporosis is a metabolic skeletal disorder characterized by increased fragility and fracture risk as s result of reduced bone mineral density and microstructural destruction and caused a heavy burden on families and society. Current medicines, on the other hand, have some limitations, with side effects and doubts regarding long-term efficacy being highlighted. Studies seeking for natural constituents as potential treatment options therefore come into focus. Icariin is a phytochemical derived from a traditional Chinese medicine, Herba epimedium, that has been used to treat orthopedic disorders in ancient China for thousands of years, including osteoporosis, osteoarthritis, and fracture. Icariin belongs to a category of prenylated flavonoids and has been shown to help reduce osteoporosis bone loss while having relatively low side effects. Icariin's anti-osteoporosis properties manifest in a variety of ways, like promoting osteogenesis, suppressing osteoclastogenesis and bone resorption, regulating migration, proliferation, and differentiation of mesenchymal stem cells, enhancing angiogenesis, anti-inflammation, and antioxidation. These procedures entail a slew of critical signaling pathways, such as PPARγ, ERα/AKT/β-catenin, and MAPK. Therefore, icariin can be an applicable alternative to improve osteoporosis although the underlying mechanisms have yet to be fully understood. In this study, we searched using the terms “icariin” and “osteoporosis,” and included 64 articles meeting the inclusion criteria and reviewed the research of icariin in anti-osteoporosis over the last 10 years, and discussed new prospects for future study. Therefore, this review may provide some references for further studies.

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Icariin triggers osteogenic differentiation of bone marrow stem cells by up-regulating miR-335–5p

Cited by 15 publications

References 33 publications

Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation

Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation

Therapeutic approach of natural products that treat osteoporosis by targeting epigenetic modulation

Icariin: A Potential Alternative Against Osteoporosis

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