Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation

Wu, Zhidi; Ou, Ling; Wang, Chaopeng; Yang, Li; Wang, Panpan; Liu, Hengrui; Xiong, Yingquan; Sun, Kai; Zhang, Ronghua; Zhu, Xiaofeng

doi:10.1016/j.biopha.2017.07.071

Cited by 75 publications

(44 citation statements)

References 37 publications

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“…As in vivo evidence for activation of p38 MAPK in osteoblast differentiation, several reports have shown that p38 MAPK knock-out transgenic mice have impaired osteoblast differentiation, bone composition, and maintenance [39,40]. As described in the results section of our study ( Figure 3A and 3B), p38 MAPK is phosphorylated by metformin treatment to show stronger binding to Runx2 [20] and OSX [21], which seems to increase their transcriptional activity during osteoblastic differentiation. Previously, Ge et al demonstrated that p38 MAPK phosphorylates Ser319 of Runx2 using a specific phospho-Runx2 antibody and activates the transcriptional activity of Runx2 according to luciferase assay analysis, leading to osteoblast differentiation [41].…”

Section: Agingsupporting

confidence: 81%

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Metformin coordinates osteoblast/osteoclast differentiation associated with ischemic osteonecrosis

Park

Kang

Moon

et al. 2020

Aging

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Ischemic necrosis of the femoral head (INFH) can lead to permanent femoral head deformity, severely compromised hip joint longevity, and premature endstage osteoarthritis as early as the third decade of life. Use of biological agents to preserve the femoral head and avoid joint replacement surgery is currently under investigation. The therapeutic concepts of angiogenesis and secondary osteogenesis have gained considerable attention in terms of preventing development of femoral head deformities after ischemic osteonecrosis [1]. Thus far, several kinds of growth factors have been reported to regulate angiogenesis [2]. Among them, Angiopoietin 1 (Ang1) is a well-known angiogenic factor that plays a pivotal role in stimulating and developing new vasculature for bone formation [3, 4].

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Section: Agingsupporting

confidence: 81%

“…p38 MAPK is reported to interact physically with and transcriptionally activate Runx2 [20] and OSX [21] during osteoblastic differentiation. Thus, we examined whether metformin can induce phosphorylation (activation) of p38 MAPK in U2OS and MG63 cells.…”

Section: Metformin Induces Phosphorylation Of P38mentioning

confidence: 99%

Metformin coordinates osteoblast/osteoclast differentiation associated with ischemic osteonecrosis

Park

Kang

Moon

et al. 2020

Aging

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“…On the other hand, icaritin, a hydrolytic product of icariin from the genus Epimedium, can significantly upregulate ERK and p38 signaling in itsinduced osteogenic differentiation in MC3T3-E1 pre-osteoblasts. Furthermore, p38 antagonist SB203580 and ERK1/2 antagonist PD98059 markedly inhibit the icaritin-induced the mRNA expression of ALP, COL1 (encoding collagen type I), OCN and OPN (encoding osteopontin) (Wu et al, 2017). These results suggest that different ligands may stimulate different patterns of signaling.…”

Section: Discussionmentioning

confidence: 81%

Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces

Sun

Bolscher

et al. 2020

Front. Bioeng. Biotechnol.

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Promoting cell spreading is crucial to enhance bone healing and implant osteointegration. In this study, we investigated the stimulatory effect of human salivary histatin-1 (Hst-1) on the spreading of osteogenic cells in vitro as well as the potential signaling pathways involved. Osteogenic cells were seeded on bio-inert glass slides with or without the presence of Hst1 in dose-dependent or time-course assays. 1 scrambled and 6 truncated Hst1 variants were also evaluated. Cell spreading was analyzed using a well-established point-counting method. Fluorescent microscopy was adopted to examine the cellular uptake of fluorescently labeled Hst1 (F-Hst1) and also the cell spreading on sandblasted and acid etched titanium surfaces. Signaling inhibitors, such as U0126, SB203580, and pertussis toxin (PTx) were used to identify the potential role of extracellular-signal-regulated kinase, p38 and G protein-coupled receptor pathways, respectively. After 60 min incubation, Hst1 significantly promoted the spreading of osteogenic cells with an optimal concentration of 10 µM, while truncated and scrambled Hst1 did not. F-Hst1 was taken up and localized in the vicinity of the nuclei. U0126 and SB2030580, but not PTx, inhibited the effect of Hst1. 10 µM Hst1 significantly promoted the spreading of osteogenic cells on both bio-inert substrates and titanium SLA surfaces, which involved ERK and p38 signaling. Human salivary histatin-1 might be a promising peptide to enhance bone healing and implant osteointegration in clinic.

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“…The broad anticancer activity of PAQR3 is mediated through the alteration of multiple different signaling pathways ( 20 ). PAQR3 overexpression decreased the phosphorylation of ERK1/2 in ESCC cells, without affecting the activation of Akt ( 21 ). PAQR3 has demonstrated the ability to interfere with multiple aspects of tumor biology, including proliferation, migration, invasion, and EMT ( 22 , 23 ).…”

Section: Discussionmentioning

confidence: 99%

Suppressor PAQR3 associated with the clinical significance and prognosis in esophageal squamous cell carcinoma

et al. 2018

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Progestin and adipoQ receptor family member 3 (PAQR3) is a novel tumor suppressor; however, its function in esophageal cancer is not well understood. The present study explored the association between PAQR3, and the survival and clinical phenotype in patients with esophageal squamous cell carcinoma (ESCC). The expression of PAQR3 in 80 cases of ESCC and its corresponding adjacent tissues was detected by reverse transcription-quantitative polymerase chain reaction. The results demonstrated that PAQR3 expression in cancer tissues was significantly lower compared with that in adjacent tissues. Clinicopathological analysis indicated that PAQR3 expression was significantly correlated with ethnicity (P=0.032), tumor length (P=0.019), lymph node metastasis (P=0.011) and local recurrence (P=0.009). Notably, the Kaplan-Meier survival curve demonstrated that a decrease in PAQR3 expression was associated with poor prognosis in patients with ESCC. Multivariate analysis indicated that PAQR3 expression was an independent prognostic indicator for patients with ESCC. PAQR3 may serve an important role in the progress of ESCC and become a potential candidate for ESCC targeted therapy.

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Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation

Cited by 75 publications

References 37 publications

Metformin coordinates osteoblast/osteoclast differentiation associated with ischemic osteonecrosis

Metformin coordinates osteoblast/osteoclast differentiation associated with ischemic osteonecrosis

Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces

Suppressor PAQR3 associated with the clinical significance and prognosis in esophageal squamous cell carcinoma

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