Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L

Li, P.; Hulák, Martin; Koubek, Petr; Šulc, Miroslav; Dzyuba, Borys; Boryshpolets, Sergii; Rodina, Marek; Gela, David; Maňásková-Postlerová, Pavla; Pěknicová, Jana; Linhart, Otomar

doi:10.1016/j.theriogenology.2010.02.024

Cited by 77 publications

(58 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Owing to the freezing/thawing processes, antioxidant enzyme levels in the spermatozoa and the seminal plasma decrease, making them more sensitive to spermatozoal lipid peroxidation. As a result permanent damages occur on spermatozoa membranes and all functions of the spermatozoa are negatively affected (19,29).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (Oncorhynchus Mykiss) Semen

Ekici¹,

Baran²,

Yamaner³

et al. 2012

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

“…Spermatozoa are affected by many factors during cryopreservation, such as temperature change in the environment, osmotic stress, toxic effect of cryoprotectants and forming of ice crystals (19,20,29). Oxidative stress is another important stress factor which the spermatozoa are exposed to (18,29).…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (Oncorhynchus Mykiss) Semen

Ekici¹,

Baran²,

Yamaner³

et al. 2012

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

“…Freezing and thawing processes can cause changes in spermatozoa protein profiles that may lead to a decreased in semen quality and affect DNA integrity (LI et al, 2010), compromising the ability of fertilization. This negative effect on sperm could be observed in the present research, which shows a reduction of almost 50% in the rate of motility compared to fresh semen.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Prochilodus lineatus semen: effect of cryoprotectants combination

et al. 2015

View full text Add to dashboard Cite

The study was conducted with the objective of comparing the toxicity and the effect of the combination of intra and extracellular cryoprotectants in curimba Prochilodus lineatus sperm cells subjected to cryopreservation. Semen from 19 males were analyzed and diluted in four solutions comprise of intra and extracellular cryoprotectants in the following combinations: methanol+lactose, methanol+egg yolk, DMSO+lactose and DMSO+egg yolk. A portion of the diluted semen was frozen while the remaining fraction was kept in repose and evaluated after 10 min. For freezing, the diluted samples were packaged in 0.50 mL straws and placed into liquid nitrogen vapor for 24 h and, after this time, submerged into liquid nitrogen for 10 days. The combination of DMSO+lactose was less toxic to the diluted semen, resulting in higher motility rates and durations when compared to the other treatments. After thawing, the highest motility rate and duration were obtained using lactose as extracellular cryoprotectant, regardless of its combination. There was no significant difference between treatments when analyzing sperm morphology after thawing. Considering the effects of the tested treatments, the use of lactose as an extracellular cryoprotectant added with DMSO or methanol is the most suitable, since these combinations presented the highest motility rates and durations and low morphological change rate after thawing.Keywords: genetic preservation; cryobiology; sperm analysis; freezing protocol; toxicity; curimba CRIOPRESERVAÇÃO DO SÊMEN DO Prochilodus lineatus: EFEITO DA COMBINAÇÃO DE CRIOPROTETORES RESUMOO estudo foi realizado com o objetivo de comparar a toxicidade e o efeito da combinação de crioprotetoresintra e extracelulares em espermatozóides de curimba Prochilodus lineatus submetidos à criopreservação. O sêmen de 19 machos foi analisado e diluído em quatro soluções com crioprotetores intra e extracelulares nas seguintes combinações: metanol+lactose, metanol+gema de ovo, DMSO+lactose e DMSO+gema de ovo. Uma porção do sêmen diluído foi congelada enquanto que a fração remanescente foi mantida em repouso e avaliada após 10 min. Para o congelamento, as amostras diluídas foram envasadas em palhetas de 0,50 mL e colocadas em vapor de nitrogênio líquido durante 24 h e, após este tempo, submersas em nitrogênio líquido durante 10 dias. A combinação de DMSO+lactose se mostrou menos tóxica para o sêmen diluído, resultando em taxas mais elevadas de motilidade e duração espermática quando comparadas com os outros tratamentos. Após descongelamento, as maiores taxas de motilidade e duração foram obtidas usando lactose como crioprotector extracelular, independentemente da sua combinação. Não houve diferença significativa entre os tratamentos ao analisar a morfologia espermática após a descongemento. Considerando-se os efeitos dos tratamentos utilizados, o uso de lactose como crioprotector extracelular, adicionada ao DMSO ou metanol, é o mais adequado, uma vez que estas combinações apresentaram maiores taxas de motilidade e duração espe...

show abstract

“…However, failures in terms of sperm motility and viability among studies may be partly attributed to cryoinjury and/or oxidative damage to spermatozoa during cryopreservation, the latter, by generation of reactive oxygen species (Basavaraja & Hegde 2004). Oxidative damage during sperm cryopreservation has been known to induce lipid peroxidation, which affected spermatozoa structure and function and DNA damage (Watson 2000;Chen et al 2010;Li, Hulak, Koubek, Sulc, Dzyuba, Boryshpolets, Rodina, Gela, Manaskova-Postlerova, Peknicova & Linhart 2010). Further research about effect of oxidative stress during long-term storage of cryopreserved B. gonionotus spermatozoa on DNA damage should be explored.…”

Section: Effect Of Storage Period Of Cryopreserved Sperm On Post-thawmentioning

confidence: 99%

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

et al. 2014

View full text Add to dashboard Cite

Effectiveness and efficiency of frozen sperm on fertilization and hatching success of eggs from silver barb was examined in relation to cryoprotectants, freezing rate and storage period. Sperm was diluted in calcium-free Hank's balanced salt solution, equilibrated with dimethylsulphoxide (DMSO), propylene glycol, sucrose or methanol at 5%, 10%, 15% or 20% final concentrations, and frozen in 250-lL straws using a one-step freezing procedure (1, 5 and 8°C min À1 from 25 to À40°C). Highest post-thaw sperm motility was found from a treatment using 10% DMSO and 5°C min À1 (82.2 AE 2.1%), similar to that of 10% DMSO and 8°C min À1 (87.8 AE 3.2%). Post-thaw motility of sperm frozen at 5 or 8°C min À1 was significantly higher than 1°C min À1 . Relative sperm motility declined significantly after 10 months of cryostorage while viability did not change during a 12-month cryostorage. Average fertilization rates of sperm after 1 and 4 months of storage were 64.5 AE 4.6% and 61.3 AE 3.4%, respectively, similar to those of fresh sperm (69.6-72.3%). Hatching rates of cryopreserved sperm (45.4-51.2%) were similar to those of fresh sperm (51.8-57.8%). This study developed suitable methods for cryopreservation of silver barb sperm that can be used to facilitate hatchery operation.

show abstract

Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L

Cited by 77 publications

References 28 publications

Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (Oncorhynchus Mykiss) Semen

Effects of Different Doses of Taurine in the Glucose-Based Extender During Cryopreservation of Rainbow Trout (Oncorhynchus Mykiss) Semen

Cryopreservation of Prochilodus lineatus semen: effect of cryoprotectants combination

Sperm cryopreservation of silver barb (Barbodes gonionotus): cryoprotectants, cooling rate and storage time on sperm quality

Contact Info

Product

Resources

About