ICE
            <i>Sp1116</i>
            , the Genetic Element Responsible for
            <i>erm</i>
            (B)-Mediated, Inducible Resistance to Erythromycin in Streptococcus pyogenes

Brenciani, Andrea; Tiberi, Erika; Morici, Eleonora; Oryaşin, Erman; Giovanetti, Eleonora; Varaldo, Pietro E.

doi:10.1128/aac.01494-12

Cited by 11 publications

(5 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…gallolyticus strain DSM 16831 was increased after adhesion to collagen. One region consists of transposon genes, and has similarities to ICE Sp 1116 of Streptococcus pyogenes [ 60 , 61 ]. The phage genes whose expression was upregulated through collagen adhesion (the second region) have similarities to the streptococcal bacteriophage P9 [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

et al. 2018

View full text Add to dashboard Cite

Streptococcus gallolyticus subsp. gallolyticus is a commensal bacterium of the human gastrointestinal tract, and a pathogen causing infective endocarditis and other biofilm-associated infections via exposed collagen. This study focuses on the characterization of the biofilm formation and collagen adhesion of S. gallolyticus subsp. gallolyticus under different conditions. In this study, it has been observed that the isolate UCN 34 is resistant to 20 mg/ ml lysozyme in BHI medium, whereas the strain BAA-2069 builds more biofilm in the presence of lysozyme compared to in a control of BHI without lysozyme. A transcriptome analysis with whole genome microarrays of these two isolates in BHI medium with lysozyme compared to control without lysozyme revealed changes in gene expression levels. In the isolate BAA-2069, 67 genes showed increased expression in the presence of lysozyme, while in the isolate UCN 34, 165 genes showed increased expression and 30 genes showed decreased expression through lysozyme treatment. Products of genes which were higher expressed are in involved in transcription and translation, in cell-wall modification, in hydrogen peroxide resistance and in bacterial immunity. Furthermore, the adhesion ability of different strains of S. gallolyticus subsp. gallolyticus to collagen type I and IV was analyzed. Thereby, we compared the adhesion of 46 human isolates with 23 isolates from animals. It was shown that the adhesion ability depends significantly on whether the isolate was isolated from human or animal. For example, high adhesion ability was observed for strain UCN 34 isolated from an infective endocarditis patient, whereas strain DSM 16831 isolated from koala feces adhered only marginally to collagen. Full genome microarray analysis of these two strains revealed strain-dependent gene expression due to adhesion. The expression of 25 genes of a transposon and 15 genes of a phage region in strain DSM 16831 were increased, which corresponds to horizontal gene transfer. Adherence to collagen in strain UCN 34 led to higher expression of 27 genes and lower expression of 31 genes. This was suggestive of a change in nutrient uptake.

show abstract

Section: Discussionmentioning

confidence: 99%

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

et al. 2018

View full text Add to dashboard Cite

show abstract

“…for which at least one complete genome was available, with S. suis being the most "colonized" species (184,185). In human clinical streptococcal isolates, erm and tet resistance genes were recurrently reported on ICEs, such as erm(B) on ICESp1116 and erm(TR)-tet(O) on ICESp2905 in S. pyogenes (186,187), erm(TR) on ICESagTR7 in S. agalactiae (188), and erm(B) and tet(O) on ICESsD9 in S. suis (189). Interestingly, resistance genes can also be mobilized by coresident ICEs, as demonstrated by the mobilization of an erm(T)-carrying plasmid in S. dysgalactiae subsp.…”

Section: Role Of the Ices In The Evolution Of Resistancementioning

confidence: 99%

Antimicrobial Resistance inStreptococcusspp

2018

View full text Add to dashboard Cite

The genus includes Gram-positive organisms shaped in cocci and organized in chains. They are commensals, pathogens, and opportunistic pathogens for humans and animals. Most species of veterinary relevance have a specific ecological niche, such as , which is almost exclusively an environmental pathogen causing bovine mastitis. In contrast, can be considered as a true zoonotic pathogen, causing specific diseases in humans after contact with infected animals or derived food products. Finally, species such as can be sporadically zoonotic, even though they are pathogens of both humans and animals independently. For clarification, a short taxonomical overview will be given here to highlight the diversity of streptococci that infect animals. Several families of antibiotics are used to treat animals for streptococcal infections. First-line treatments are penicillins (alone or in combination with aminoglycosides), macrolides and lincosamides, fluoroquinolones, and tetracyclines. Because of the selecting role of antibiotics, resistance phenotypes have been reported in streptococci isolated from animals worldwide. Globally, the dynamic of resistance acquisition in streptococci is slower than what is experienced in , probably due to the much more limited horizontal spread of resistance genes. Nonetheless, transposons or integrative and conjugative elements can disseminate resistance determinants among streptococci. Besides providing key elements on the prevalence of resistance in streptococci from animals, this article will also largely consider the mechanisms and molecular epidemiology of the major types of resistance to antimicrobials encountered in the most important streptococcal species in veterinary medicine.

show abstract

“…In addition, mef(A) can be associated with other MGEs in GAS such as ΦM46.1, which also harbors the tet(O) gene (Brenciani et al, 2010). Numerous other ICEs (or plasmids) present in GAS harbor additional antibiotic resistance genes including tet(M), erm(A) and erm(B) (Banks et al, 2004a; Beres and Musser, 2007; Brenciani et al, 2012; Giovanetti et al, 2012; Tse et al, 2012; Varaldo et al, 2009; Willems et al, 2011; Woodbury et al, 2008). It was estimated that resistance to macrolides was acquired by GAS in at least 49 independent genetic events (Robinson et al, 2006), and tetracycline resistance has arisen ≥80 times (Ayer et al, 2007); both values may be gross underestimates.…”

Section: Mobile Genetic Elementsmentioning

confidence: 99%

Molecular epidemiology and genomics of group A Streptococcus

Bessen

McShan

Nguyen

et al. 2015

Infection, Genetics and Evolution

View full text Add to dashboard Cite

Streptococcus pyogenes (group A streptococcus; GAS) is a strict human pathogen with a very high prevalence worldwide. This review highlights the genetic organization of the species and the important ecological considerations that impact its evolution. Recent advances are presented on the topics of molecular epidemiology, population biology, molecular basis for genetic change, genome structure and genetic flux, phylogenomics and closely related streptococcal species, and the long- and short-term evolution of GAS. The application of whole genome sequence data to addressing key biological questions is discussed.

show abstract

ICE Sp1116 , the Genetic Element Responsible for erm (B)-Mediated, Inducible Resistance to Erythromycin in Streptococcus pyogenes

Cited by 11 publications

References 35 publications

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

Biofilm formation and transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in response to lysozyme

Antimicrobial Resistance inStreptococcusspp

Molecular epidemiology and genomics of group A Streptococcus

Contact Info

Product

Resources

About