Ice-Nucleating Active Bacteria Reduce the Cold-Hardiness of the Freeze-Intolerant Colorado Potato Beetle (Coleoptera: Chrysomelidae)

Lee, Richard; Costanzo, Jon P.; Kaufman, Phillip E.; Lee, Marcia R.; Wyman, J. A.

doi:10.1093/jee/87.2.377

Cited by 28 publications

(25 citation statements)

References 6 publications

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“…Castrillo et al (2000) reported that the presence of certain ice-nucleating bacterial strains in the gut of diapausing Colorado potato beetles, Leptinotarsa decemlineata (Say), signiÞcantly elevated their supercooling points. Lee et al (1994) reported the presence of a particular strain of bacteria, Pseudomonas syringae, in the Colorado potato beetle gut elevated the insectÕs supercooling point as much as 4ЊC. Authors in both of these studies noted that the magnitude of elevation was related to the quantity of bacteria present in the gut.…”

Section: Resultsmentioning

confidence: 99%

Influence of Feeding Status and Physiological Condition on Supercooling Points of Adult Boll Weevils (Coleoptera: Curculionidae)

2002

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Section: Resultsmentioning

confidence: 99%

Influence of Feeding Status and Physiological Condition on Supercooling Points of Adult Boll Weevils (Coleoptera: Curculionidae)

2002

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“…For example, freeze-avoidant species may be susceptible to conditions that promote ice formation, such as the application of ice nucleators (e.g. Fields, 1993;Lee et al, 1994). …”

Section: Cold Tolerance Strategymentioning

confidence: 99%

An invitation to measure insect cold tolerance: Methods, approaches, and workflow

Sinclair

Alvarado

Ferguson

2015

Journal of Thermal Biology

313

326

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Insect performance is limited by the temperature of the environment, and in temperate, polar, and alpine regions, the majority of insects must face the challenge of exposure to low temperatures. The physiological response to cold exposure shapes the ability of insects to survive and thrive in these environments, and can be measured, without great technical difficulty, for both basic and applied research. For example, understanding insect cold tolerance allows us to predict the establishment and spread of insect pests and biological control agents. Additionally, the discipline provides the tools for drawing physiological comparisons among groups in wider studies that may not be focused primarily on the ability of insects to survive the cold. Thus, the study of insect cold tolerance is of a broad interest, and several reviews have addressed the theories and advances in the field. Here, however, we aim to clarify and provide rationale for common practices used to study cold tolerance, as a starter's guide for newcomers to the field, students, and those wishing to incorporate cold tolerance into a broader study. We cover the 'tried and true' measures of insect cold tolerance, the equipment necessary for these measurement, and summarize the ecological and biological significance of each. Additionally, we provide a suggested framework and workflow for measuring cold tolerance and low temperature performance in insects.

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“…There are diverse data concerning the supercooling ability (from −3 to −20 °C) of Colorado potato beetles estimated by different authors (Lee et al, 1994;Boiteau, Coleman, 1996;Hiiesaar et al, 2001). Still these earlier studies have demonstrated that the supercooling ability of many insects is not completely associated with their cold tolerance because of the prefreeze mortality above the supercooling points (Nedvěd, 2000;Koštal et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Cold tolerance of Colorado potato beetle (Leptinotarsa decemlineata Say) adults and eggs

Hiiesaar

Karise²,

Williams³

et al. 2014

Zemdirbyste-Agriculture

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The cold tolerance of different seasonal groups (pre-diapause, diapauses and post-diapause) of Colorado potato beetle (Leptinotarsa decemlineata Say) was assessed by exposing them either to a constant temperature of -3°C for 48 to 624 h, or to -3, −4, −5, −6 or −7 °C for a constant 24 h. Cold survival was affected by the length of exposure, by the temperature rates and by seasonality. The least cold-tolerant were pre-diapause beetles in August with a mean lethal temperature (Ltemp50) of −4.4°C for 24 h exposure and with a lethal time (Ltime50) of 106.8 h at −3°C. Cold tolerance was significantly higher in the diapause group in January and in the post-diapause group in March; it did not differ significantly between these groups of beetle for 24 h exposure, the lethal temperature being −5.8°C for both groups. Seasonality had a greater influence on survival of these groups after longer exposure to constant mild sub-zero temperature (−3°C); in diapause group, all the beetles survived 624 h exposure, but in post-diapause group the survival limit decreased to 216 h and lethal time was 153.9 h. Colorado potato beetle eggs tolerated 24 h exposure to sub-zero temperatures from −3°C to −5°C or longer exposure at −3°C for 72 h without a reduction in hatchability. The mean lethal time for 50% mortality of eggs was 76.5 h and mean lethal temperature was −5.8°C. Thus, we conclude that night frosts common in northern regions do not damage Colorado potato beetle adults or eggs to the extent that might threaten the survival of the population.

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Ice-Nucleating Active Bacteria Reduce the Cold-Hardiness of the Freeze-Intolerant Colorado Potato Beetle (Coleoptera: Chrysomelidae)

Cited by 28 publications

References 6 publications

Influence of Feeding Status and Physiological Condition on Supercooling Points of Adult Boll Weevils (Coleoptera: Curculionidae)

Influence of Feeding Status and Physiological Condition on Supercooling Points of Adult Boll Weevils (Coleoptera: Curculionidae)

An invitation to measure insect cold tolerance: Methods, approaches, and workflow

Cold tolerance of Colorado potato beetle (Leptinotarsa decemlineata Say) adults and eggs

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