The fauna of ichneumonid wasps (Hymenoptera: Ichneumonidae) from Isfahan province (Iran) is studied in this paper. In total 28 species from 24 genera and 6 subfamilies (Campopleginae, Cryptinae, Ichneumoninae, Ophioninae, Pimplinae and Tryphoninae) were collected and identified. Encrateola laevigata (Ratzeburg, 1848), Mesoleptus laticintus (Walker, 1874) (Cryptinae), Hepiopelmus melanogaster (Gmelin, 1790) (Ichneumonidae), and Apechthis quadridentata (Thomson, 1877) (Pimplinae) are new records for the fauna of Iran.