ICP-MS trace element analysis in serum and whole blood

Laur, Nico; Kinscherf, Ralf; Pomytkin, Karolina; Kaiser, Lars; Knes, Otto; Deigner, Hans‐Peter

doi:10.1371/journal.pone.0233357

Cited by 92 publications

(57 citation statements)

References 33 publications

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“…The ID analysis was based on the isotope shift of the electronic transition 2 2 P←2 2 S, which exhibits two spinorbit components for each isotope, as we previously described [21]. The nonlinear fit parameters were optimized using the spectra of 7 Li-and 6 Li-enriched materials. The wavelength of the spectra was corrected by using La as an internal spectral standard for the electronic transition 5d2(3F)6p←5d2(3F)6s as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Since the four peaks reflect only two doublet transitions of the two isotopes, the ratio between two respective areas (e.g., A1 and A2) is constant. Namely, the spectral displacement between c1 and c3 is the isotopic shift of 15.80 pm between 7 Li and 6 Li [22]. The distance between c1 and c2 or c3 and c4 is the spin-orbit splitting of 15.08 pm.…”

Section: Discussionmentioning

confidence: 99%

“…Some attractive alternatives to ID-MS techniques are based on the isotope shift in atomic and molecular electronic spectra. For example, ID-AAS was proposed by Brost et al to monitor the absorption coefficient of naturally occurring Li in human plasma and enriched isotopic materials using natural and 6 Li-enriched hollow cathode lamps. The bias for Li recovery in plasma ranged from -2.8% to 0.6% [15].…”

Section: Several Analytical Methods For the Quantification Of LI In Bmentioning

confidence: 99%

“…Each sample was measured ten times, and 150 spectra were recorded during each atomization. The same procedure was applied for the 6 Li spike solution and the spiked standard solution (used for reverse ID) and for the 7 Li solution. Between each sample, a blank sample was measured five times.…”

Section: Atomic Absorption Spectrometry Measurementsmentioning

confidence: 99%

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Determination of Lithium in Human Serum by Isotope Dilution Atomic Absorption Spectrometry

Winckelmann¹,

Morcillo²,

Richter³

et al. 2021

Preprint

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The therapeutic dose of lithium (Li) compounds, which are widely used for the treatment of psychiatric and hematologic disorders, is close to its toxic level; therefore, drug monitoring protocols are mandatory. Herein, we propose a fast, simple, and low-cost analytical procedure for the traceable determination of Li concentration in human serum, based on the monitoring of the Li isotope dilution through the partially resolved isotope shift in its electronic transition around 670.80 nm using a commercially available high-resolution continuum source graphite furnace atomic absorption spectrometer. With this technique, serum samples only require acidic digestion before analysis. The procedure requires three measurements—an enriched 6Li spike, a mixture of a certified standard solution and spike, and a mixture of the sample and spike with a nominal 7Li/6Li ratio of 0.82. Lanthanum has been used as an internal spectral standard for wavelength correction. The spectra are described as the linear superposition of the contributions of the respective isotopes, each consisting of a spin-orbit doublet, which can be expressed as Gaussian components with constant spectral position and width and different relative intensity, reflecting the isotope ratio in the sample. Both, the spectral constants and the correlation between isotope ratio and relative band intensity have been experimentally obtained using commercially available materials enriched with Li isotopes. The procedure has been validated using five human serum certified reference materials. The results are metrologically comparable and compatible to the certified values. The measurement uncertainties are comparable to those obtained by the more complex and expensive technique, isotope dilution mass spectrometry.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Several Analytical Methods For the Quantification Of LI In Bmentioning

confidence: 99%

Section: Atomic Absorption Spectrometry Measurementsmentioning

confidence: 99%

See 2 more Smart Citations

Determination of Lithium in Human Serum by Isotope Dilution Atomic Absorption Spectrometry

Winckelmann¹,

Morcillo²,

Richter³

et al. 2021

Preprint

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“…6,7 For trace element analysis, instrumented approaches such as inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectroscopy (ICP-OES) are suitable and go-to tools. [8][9][10] Amongst the two techniques, ICP-MS is the more sensitive approach, and is able to detect, with precision, parts per billion (ppb) concentration of many metals and metalloids in the Periodic table. On the other hand, ICP-OES is a more robust and less maintenance intensive instrument for routine detection of elements in the parts per million (ppm) range in more difficult matrixes.…”

Section: Introductionmentioning

confidence: 99%

ICP-MS analysis of metal and metalloid concentrations of common microbiological growth media reveals presence of heavy metals

2020

Preprint

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Trace elements such as cobalt and manganese play important roles as cofactors of enzymes, and thus, they collectively impact on biochemistry and cellular metabolism. Hence, it is of importance to gain an understanding of the trace metal and metalloid concentrations of growth medium in order to fully account for the growth performance of the bacterium. But, this aspect of microbial cell cultivation is usually neglected. Advent of instrumented techniques for metals and metalloid analysis such as inductively coupled plasma mass spectrometry (ICP-MS) has significantly facilitated trace element analysis at the parts per billion (ppb) to parts per million (ppm) level in aqueous matrixes free of organic interference. In this work, ICP-MS was utilized as the principal tool for profiling the range of metals and metalloids in different microbiological growth medium ranging from minimal salts medium to complex chemically undefined medium. Growth media examined include: LB Lennox, LB Lennox + 2 g/L glucose, LB Lennox (buffered, 89 mM phosphate), LB Lennox (buffered, 89 mM phosphate) + 6 g/L glucose, formulated medium + 6 g/L glucose, Tryptic Soy Broth, M9 medium, and M9 + 1 g/L yeast extract medium. Results reveal detection of elevated concentrations of chromium, copper and cadmium in different versions of LB Lennox and Tryptic Soy Broth. Concentrations of the above heavy metals in the tens of ppm range meant that the growth media could only support the growth of environmental bacteria with some resistance to heavy metal toxicity. On the other hand, no heavy metals were detected in M9 minimal salts medium or a modified version with supplementation of 1 g/L yeast extract. This indicated that minimal salts media may have less of a problem with heavy metal contamination compared to chemically undefined microbiological growth media. Collectively, the results highlight the essentiality of conducting a comprehensive profiling experiment for detecting different metals and metalloids at trace levels in microbiological growth medium. Such data would in addition to offering a deeper understanding of some peculiar growth behaviour from some microorganisms, may also help identify contamination issue during manufacture that preclude use of the media in cultivating many laboratory domesticated microorganisms.Subject areasbiochemistry, biotechnology, cell biology, microbiology, biochemical engineering,

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