Id: 158

Pfefferkorn, Cathleen; Kallfass, Carsten; Lienenklaus, Stefan; Spanier, Julia; Kalinke, Ulrich; Rieder, Martina; Conzelmann, Karl‐Klaus; Michiels, Thomas; Staeheli, Peter

doi:10.1016/j.cyto.2015.08.178

Cited by 1 publication

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“…In other cases, experimental ablation of microglia exacerbated inflammation and worsen neural injuries (Lalancette-Hebert et al, 2007;Rubino et al, 2018). On the other hand, astrocytes have been reported as the main cell type for IFNβ production upon virus infection in vivo (Lindqvist et al, 2016;Pfefferkorn et al, 2016;Chhatbar et al, 2018). However, due to the complexity of the interaction between microglia and astrocytes, it would be very difficult to identify the exact cell type from which the immune responses were originated if the microglia and astrocytes were not completely separated from each other.…”

Section: Introductionmentioning

confidence: 99%

A novel histone deacetylase inhibitor-based approach to eliminate microglia and retain astrocyte properties in glial cell culture

Huang

et al. 2021

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The close association between astrocytes and microglia causes great difficulties to distinguish their individual roles in innate immune responses in central nervous system. Current chemical-based methods to eliminate microglia in glial cell culture introduce various molecular and functional alterations to astrocytes. Here, we describe a novel two-step approach to achieve a complete elimination of microglia without affecting the biological properties of co-cultured astrocytes by temporal treatment of histone deacetylase inhibitor trichostatin A (TSA). We verify TSA as a potent inducer for microglial-specific cell death, which also causes comprehensive gene expression changes in astrocytes. However, withdrawal of TSA not only ensures no microglia repopulation, but also restores all the gene expression changes in terms of astrocyte functions, including neurotrophic factors, glutamate and potassium transporters, and reactive astrocyte subtypes. By contrast, withdrawal of PLX5622, the commonly used colony-stimulating factor 1 receptor inhibitor neither prevents microglia repopulation nor restores the gene expression changes mentioned above. Using this method, we are able to discriminate differential roles of microglia and astrocytes in the induced expression of antiviral and pro-inflammatory cytokines upon various pathological stimuli including the spike protein of SARS-CoV-2. This simple and efficient method can be customized for the understanding of microglia-astrocyte interaction and the development of epigenetic therapies that target over-activated microglia in neuroinflammation-related diseases.

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Section: Introductionmentioning

confidence: 99%

A novel histone deacetylase inhibitor-based approach to eliminate microglia and retain astrocyte properties in glial cell culture

Huang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

Id: 158

Cited by 1 publication

References 0 publications

A novel histone deacetylase inhibitor-based approach to eliminate microglia and retain astrocyte properties in glial cell culture

A novel histone deacetylase inhibitor-based approach to eliminate microglia and retain astrocyte properties in glial cell culture

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