Identificação de alguns constituintes químicos de Indigofera hirsuta Linn. (Fabaceae) por CLAE-IES-EM (TOF) e avaliação da atividade antirradicalar

Moura, Adriana Candido da Silva; Vilega, Wagner; Santos, Lourdes Campaner dos

doi:10.1590/s0100-40422011000700006

Cited by 19 publications

(11 citation statements)

References 21 publications

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“…Structure elucidation. The structures of purified compounds were identified by comparing their NMR data with the literature (20,21). Nuclear magnetic resonance spectra were recorded with a Bruker DRX-400 spectrometer at 300 MHz ( 1 H, COSY) and 75.5 MHz ( 13 C, DEPT) using DMSO-d 6 as the solvent.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Photoprotective Potential and Percutaneous Penetration by Photoacoustic Spectroscopy of the Schinus terebinthifolius Raddi Extract

Bulla

Hernandes

Baesso

et al. 2015

Photochem & Photobiology

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Schinus terebinthifolius is a plant rich in phenolic compounds, which have antioxidant properties and can provide new opportunities for treatment and prevention of diseases mediated by ultraviolet radiation like photoaging and skin cancer. The aim of this study was to evaluate the photoprotective potential and ex vivo percutaneous penetration of the crude extract of Schinus terebinthifolius leaves. The extract was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. The sun protection factor was also evaluated. The ex vivo skin permeation of the emulsion and gel formulations were assayed. Fractionation of the extract resulted in gallic acid, ethyl gallate and a mixture of flavonoids, suggesting derivatives of quercetin and myricetin. The phenolic content of the extract was 384.64 ± 2.60 mg GAE g(-1) extract. The antioxidant activity was superior to butylated hydroxytoluene, in DPPH method, and ascorbic acid and rutin, in β-carotene bleaching assay. The extract showed UV absorption with photoprotector potential in the UVB region. The photoacoustic spectroscopy measurements confirmed absorption in the UV region and topical application of the formulations caused no histological changes in the rats' skin. These results suggest that the crude extract of Schinus terebinthifolius leaves may be a promising natural sunscreen product.

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Section: Methodsmentioning

confidence: 99%

Evaluation of Photoprotective Potential and Percutaneous Penetration by Photoacoustic Spectroscopy of the Schinus terebinthifolius Raddi Extract

Bulla

Hernandes

Baesso

et al. 2015

Photochem & Photobiology

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“…The chromatographic fractionation of ethanol extract (2.0 g) yielded the mixture of trogonelline and betaine amino acids (15.0 mg), uracil (9 mg) and uridine (4.0 mg) (Figures ). These substances are being reported for the first time for the leaves of E. velutina; however, they were previously described in others plant species ( Balanites aegyptiaca , Indigofera hirsuta and Sinorhizobium meliloti ) and marine mussel ( Mytilus edulis ) …”

Section: Resultsmentioning

confidence: 99%

Standardized extract of Erythrina velutina Willd. attenuates schizophrenia-Like behaviours and oxidative parameters in experimental animal models

Dias

Almeida

Vasconcelos

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives To study the effects of the standardized extract from the leaves of Erythrina velutina in behavioural and oxidative parameters in the ketamine‐induced schizophrenia model. Methods Mice received ketamine (KET) or saline for 7 days. From 8th to 14th day, the animals received Erythrine (Eryt) (100, 200 or 400 mg/kg) or olanzapine (Olanz), 1 h after KET administration. At 14th day, 30 min after the last administration of KET, the open‐field and pre‐pulse inhibition (PPI) tests were performed. Then, the animals were sacrificed and the prefrontal cortex (PFC), hippocampus (HC) and striatum (ST) were dissected for the oxidative tests. Key findings Ketamine increased spontaneous locomotor activity and grooming. KET decreased the PPI, which was reversed by combining it with Eryt or olanzapine. KET decreased GSH concentration in PFC and ST this was reversed by Eryt. KET increased MDA concentration in PFC and HC this was reversed by Eryt. Eryt and Olanzapine reduced MDA concentration in ST when compared to KET group. Nitrite concentration was reduced by administration of KET in the PFC. Conclusions These results demonstrate that the standardized extract of E. velutina can prevent behavioural symptoms and oxidative stress induced by repeated doses of KET.

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“…[52][53] Para as substâncias 17 -21 além dos dados obtidos nos espectros de RMN de 1 H e 13 C foram utilizadas informações obtidas na análise dos dados de UV, LC/MS, EM e/ou comparação com dados da literatura. [54][55][56] 54 A análise do espectro de HMBC mostrou a correlação entre d H 3,81 (s) e d C 169,18 (C-7) indicando a presença de função éster em uma das substâncias da mistura. 54 Com base nas informações obtidas com os espectros de LC/EM-ESI, RMN de 1 H e 13 C, aliada à comparação com dados espectrométricos descritos na literatura, foi possível identificar as substâncias como o ácido gálico (17) e galato de metila (19) na proporção 1:2.…”

Section: Determinação Estrutural Das Substânciasunclassified

“…[54][55][56] 54 A análise do espectro de HMBC mostrou a correlação entre d H 3,81 (s) e d C 169,18 (C-7) indicando a presença de função éster em uma das substâncias da mistura. 54 Com base nas informações obtidas com os espectros de LC/EM-ESI, RMN de 1 H e 13 C, aliada à comparação com dados espectrométricos descritos na literatura, foi possível identificar as substâncias como o ácido gálico (17) e galato de metila (19) na proporção 1:2. 54 A mistura de flavonoides (20 + 21) se apresentou como um pó amorfo amarelado, solúvel em MeOH, que ao ser analisado por CCDA apresentou-se como duas manchas com Rf distintos de coloração amarelada e verde escuro quando revelada com vanilina sulfúrica e FeCl 3 , respectivamente.…”

Section: Determinação Estrutural Das Substânciasunclassified

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Phytochemical Profile and Antioxidant activity of a Lecythis pisonis Cambess. Specimen (Lecythidaceae)

Barreto¹,

Moreira²,

Gomes³

et al. 2020

Rev. Virtual Quim.

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The species Lecythis pisonis (L. pisonis) popularly known as "sapucaia" or "cumbuca de macaco", is a plant belonging to the family Lecythidaceae. This family has interesting pharmacological activities that have driven researchers to explore their species. Observing the biological potential of the species L. pisonis, this work aimed to trace the chemical profile of its leaves and to evaluate the in vitro antioxidant (DPPH and ABTS •+) and cytotoxic activity against Artemia salina Leach larvae. Qualitative tests identified the presence of triterpenes, steroids, saponins, flavonoids, and phenolics in general. The phytochemical investigation of the leaves resulted in the identification of the mixture of triterpenes: epifriedelanol and friedelin (1+2), α-amyrin and β-amyrin (4+5), squalene (3) and ursolic acid (16); the mixture of steroids: stigmasterol and β-sitosterol (6+7); α-tocopherol (8); of the mixture of esters: methyl palmitate, ethyl palmitate, ethyl 9-octadecenoate, 9,12,15-methyl octadecanoate, butyl palmitate, ethyl octadecanoate and butyl octadecanoate (9-15); the mixture of isoquercitrin and astragalin flavonoids (20+21); gallic acid and methyl gallate (17+19); gallic acid (17) and ellagic acid (18). This the first report of the identification of substances 8-15, 17-21 in the leaves of the species L. pisonis. The evaluation of the antioxidant activity of polar extracts revealed a great antioxidant potential, which may be associated with high levels of phenols and total flavonoids. The quantification of proanthocyanidin in the extract in MeOH showed a content of 8.65 mg EC/g of crude extract. The bioassay carried out with A. salina MeOH and MeOH:H 2 O extracts showed mild toxicity. A espécie Lecythis pisonis (L. pisonis), conhecida popularmente como sapucaia ou cumbuca de macaco, é uma planta pertencente à família Lecythidaceae. Esta família possui atividades farmacológicas interessantes que têm impulsionado pesquisadores a explorar suas espécies. Observando o potencial biológico da espécie L. pisonis, este trabalho teve como objetivo traçar o perfil químico de suas folhas, avaliar a atividade antioxidante in vitro (DPPH e ABTS •+) e citotóxica contra larvas de Artemia salina Leach. Os testes qualitativos identificaram a presença de triterpenos, esteroides, saponinas, flavonoides e outros compostos fenólicos. A investigação fitoquímica das folhas resultou na identificação da mistura de triterpenos: epifriedelanol e friedelina (1+2), α-amirina e β-amirina (4+5), esqualeno (3) e ácido ursólico (16); da mistura dos esteroides: estigmasterol e β-sitosterol (6+7); α-tocoferol (8); da mistura dos ésteres: palmitato de metila, palmitato de etila, 9-octadecenoato de etila, 9,12,15-octadecanoato de metila, palmitato de butila, octadecanoato de etila e octadecanoato de butila (9-15); da mistura dos flavonoides isoquercitrina e astragalina (20+21); do ácido gálico e galato de metila (17+19); do ácido gálico (17) e ácido elágico (18). Sendo este o primeiro relato da identificação das substâncias 8-15, 17-21 nas folh...

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Identificação de alguns constituintes químicos de Indigofera hirsuta Linn. (Fabaceae) por CLAE-IES-EM (TOF) e avaliação da atividade antirradicalar

Cited by 19 publications

References 21 publications

Evaluation of Photoprotective Potential and Percutaneous Penetration by Photoacoustic Spectroscopy of the Schinus terebinthifolius Raddi Extract

Evaluation of Photoprotective Potential and Percutaneous Penetration by Photoacoustic Spectroscopy of the Schinus terebinthifolius Raddi Extract

Standardized extract of Erythrina velutina Willd. attenuates schizophrenia-Like behaviours and oxidative parameters in experimental animal models

Phytochemical Profile and Antioxidant activity of a Lecythis pisonis Cambess. Specimen (Lecythidaceae)

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