Identificação sorológica e relação filogenética de Salmonella spp. de origem suína

Melo, Roberta Torres de; Guimarães, Adélia Rodrigues; Mendonça, Eliane Pereira; Coelho, Lísias; Monteiro, Guilherme Paz; Fonseca, Belchiolina Beatriz; Rossi, D. A.

doi:10.1590/s0100-736x2011001200001

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…are considered the major cause of foodborne disease in humans, and Salmonella Enteritidis is the most frequently isolated serovar in Europe, South America and Asia (Vieira et al 2009). Phylogenetic analyses show the influence of different factors in the existence and persistence of Salmonella spp in animals, such as cross-contamination among animals, environment and feed (Mello et al 2011). The virulence of Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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RESUMO.-[Detecção de genes associados à virulência em cepas de Salmonella Enteritidis isoladas de frangos na região sul do Brasil.] Salmonella spp. estão entre os principais agentes causadores de doenças transmitidas por alimentos, e o sorovar Salmonella Enteritidis é o mais frequentemente isolado no mundo. A virulência de Salmonella spp. e a sua interação com o hospedeiro são processos complexos que envolvem fatores de virulência para sobreviver às defesas do hospedeiro. O objetivo deste estudo foi detectar genes de virulência em cepas de S. Enteritidis isoladas a partir de fontes avícolas no sul do Brasil. Ensaios de PCR foram desenvolvidos para a detecção de nove genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH are considered the main agents of foodborne disease and Salmonella Enteritidis is one of the most frequently isolated serovars worldwide. The virulence of Salmonella spp. and their interaction with the host are complex processes involving virulence factors to overcome host defenses. The purpose of this study was to detect virulence genes in S. Enteritidis isolates from poultry in the South of Brazil. PCR-based assays were developed in order to detect nine genes (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH and spvC) associated with the virulence in eighty-four isolates of S. Enteritidis isolated from poultry. The invA, hilA, sivH, sefA and avrA genes were present in 100% of the isolates; lpfA and sopE were present in 99%; agfA was present in 96%; and the spvC gene was present in 92%. It was possible to characterize the isolates with four different genetic profiles (P1, P2, P3 and P4), as it follows: P1, positive for all genes; P2, negative only for spvC; P3, negative for agfA; and P4, negative for lpfA, spvC and sopE. The most prevalent profile was P1, which was present in 88% of the isolates. Although all isolates belong to the same serovar, it was possible to observe variations in the presence of these virulence-associated genes between different isolates. The characterization of the mechanisms of virulence circulating in the population of Salmonella Enteritidis is important for a better understanding of its biology and pathogenicity. The frequency of these genes and the establishment of genetic profiles can be used to determine patterns of virulence. These patterns, associated with in vivo studies, may help develop tools to predict the ability of virulence of different strains.

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Section: Introductionmentioning

confidence: 99%

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

et al. 2013

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“…O processo de identificação de sorovares também é bastante complexo e variável, pois os lotes geralmente são infectados por múltiplos tipos, sendo a ração, origem dos animais, tempo numa região, sistema de produção ou granja e transporte fatores que refletem a frequência de isolamento e o perfil (Schwarz et al, 2009). Para demonstrar a variabilidade dos perfis de sorovares presentes nos suínos abatidos pode-se comparar os estudos de Schwarz et al (2009) e Melo et al (2011) Lima et al (2016), encontraram em sua pesquisa Typhimurium, Derby, Enteritidis, Panama, Infantis e Anatum, como os sorovares de maior prevalência, demonstrando a grande variedade e quantidade dos tipos existentes.…”

Section: Resultsunclassified

Prevalência de salmonella spp. em produtos de origem suína

Araújo

Moura

Luz

et al. 2020

RSD

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The genus Salmonella spp. It belongs to the Enterobacteriaceae family and are bacteria that have a worldwide distribution, being present, especially, in animal production environments. About 1.9% of the cases of foodborne diseases caused by Salmonella are transmitted by pork meat and its derivatives, according to data from the Ministry of Health. In view of the above, this article aimed to investigate Salmonella in swine animals from growing meat trade and concern for public health. This study is an integrative review, covering scientific articles, monographs, theses and dissertations published in the databases: CAPES, SciELO and Google Scholar. Through studies, it is found that Salmonella spp. It is one of the main agents involved in foodborne diseases and pigs are considered important vehicles of these bacteria, acting as a source of infection, directly or indirectly, to humans. Finally, it can be concluded that the main objective of the study can be achieved, emphasizing the importance of reporting the cases of Salmonella contamination to the control departments, in order to direct the sanitary hygienic control measures, in order to ensure the safety of food of porcine origin and its derivatives, and the health of consumers.

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“…In the following steps such as handling, packaging, storage and transport of animal meal at room temperature, there were risks of contamination and recontamination by microorganisms that were distributed in the industry environment. It is important to consider that these studies were carried out in greases that used as raw material by-products generated from slaughtering under inspection of animal products (federal, state or municipal) (Larsen et al, 2014;Maciorowski et al, 2007;Melo et al, 2011;Moura et al, 2014;Pellegrini et al, 2015). In the European Union, Larsen et al (2014) reported a decrease in the presence of Salmonella from 0.7% to 0.5% in the diet and from 2.9% to 0.6% in meat and bone meal between 2002 and 2010.…”

Section: Resultsmentioning

confidence: 99%

Qualitative risk assessment of animal meal applied to swine production

Pellegrini

Celis

Costa

2017

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

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There are gaps in the pig production chain, particularly as regards the possible destinations of dead animals on farms. The production of animal meal presents itself as an alternative for the recycling of biological waste. The objective of this study was realize a qualitative microbiological risk assessment on animal meal in order to provide subsidies to indicate possible hazards and risks associated with the use of meal produced from dead pigs on farms. The microorganism Salmonella was the main hazard reported. The scenario tree presented 15 scenarios for contamination and recontamination of animal meal. For the first scenario was defined release and exposure risk levels as moderate, was obtained as the risk level of the occurrence moderate. The risk level of the consequence determined as low together with the level of occurrence obtained previously resulted in the final risk level low. For the second scenario defined release risk level as high and the exposure risk level as moderate, we obtained as moderate risk level of occurrence. The risk level of the consequence determined as high related to the level of occurrence previously obtained resulted in the final risk level high. From the information and scenarios considered, it was not possible to indicate the production of animal meal as a probable destination for dead animals on pig farms.

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Identificação sorológica e relação filogenética de Salmonella spp. de origem suína

Cited by 7 publications

References 12 publications

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil

Prevalência de salmonella spp. em produtos de origem suína

Qualitative risk assessment of animal meal applied to swine production

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