Identification and allelic frequencies of novel single-nucleotide polymorphisms in the DUSP1 and BTG1 genes

Suzuki, Chie; Unoki, Motoko; Nakamura, Yusuke

doi:10.1007/s100380170105

Cited by 9 publications

(4 citation statements)

References 5 publications

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“…DUSP1 gene encodes a dual specificity phosphatase and has been implicated as a mediator of tumor suppressor PTEN signaling pathway (34). The expression of DUSP1 has been shown to decrease in ovarian tumors and a novel single-nucleotide polymorphism in the DUSP1 gene has been identified (35). H3F3A mRNA is commonly used as a proliferative marker, and its level has been shown to be up-regulated in prostate cancers and colon cancers (36,37).…”

Section: Discussionmentioning

confidence: 99%

Salivary Transcriptome Diagnostics for Oral Cancer Detection

Marcus²,

Zhou

et al. 2004

Clinical Cancer Research

533

474

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Purpose: Oral fluid (saliva) meets the demand for noninvasive, accessible, and highly efficient diagnostic medium. Recent discovery that a large panel of human RNA can be reliably detected in saliva gives rise to a novel clinical approach, salivary transcriptome diagnostics. The purpose of this study is to evaluate the diagnostic value of this new approach by using oral squamous cell carcinoma (OSCC) as the proof-of-principle disease.Experimental Design: Unstimulated saliva was collected from patients (n ‫؍‬ 32) with primary T1/T2 OSCC and normal subjects (n ‫؍‬ 32) with matched age, gender, and smoking history. RNA isolation was done from the saliva supernatant, followed by two-round linear amplification with T7 RNA polymerase. Human Genome U133A microarrays were applied for profiling human salivary transcriptome. The different gene expression patterns were analyzed by combining a t test comparison and a fold-change analysis on 10 matched cancer patients and controls. Quantitative polymerase chain reaction (qPCR) was used to validate the selected genes that showed significant difference (P < 0.01) by microarray. The predictive power of these salivary mRNA biomarkers was analyzed by receiver operating characteristic curve and classification models.Results: Microarray analysis showed there are 1,679 genes exhibited significantly different expression level in saliva between cancer patients and controls (P < 0.05).Seven cancer-related mRNA biomarkers that exhibited at least a 3.5-fold elevation in OSCC saliva (P < 0.01) were consistently validated by qPCR on saliva samples from OSCC patients (n ‫؍‬ 32) and controls (n ‫؍‬ 32). These potential salivary RNA biomarkers are transcripts of IL8, IL1B, DUSP1, HA3, OAZ1, S100P, and SAT. The combinations of these biomarkers yielded sensitivity (91%) and specificity (91%) in distinguishing OSCC from the controls.Conclusions: The utility of salivary transcriptome diagnostics is successfully demonstrated in this study for oral cancer detection. This novel clinical approach could be exploited to a robust, high-throughput, and reproducible tool for early cancer detection. Salivary transcriptome profiling can be applied to evaluate its usefulness for other major disease applications as well as for normal health surveillance.

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Section: Discussionmentioning

confidence: 99%

Salivary Transcriptome Diagnostics for Oral Cancer Detection

Marcus²,

Zhou

et al. 2004

Clinical Cancer Research

533

474

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“…Are certain inflammatory diseases such as rheumatoid arthritis, Crohn’s disease, and psoriasis associated with defects in MKP-1 induction, as has been shown for sarcoidosis? Do MKP-1 polymorphisms previously identified in diseases such as ovarian cancer (Suzuki et al 2001) affect the cancer prognosis or sensitivity to chemotherapeutic drugs? Addressing these and related questions will help move MKP-1-based therapeutics from the lab into the clinic.…”

Section: Concluding Thoughtsmentioning

confidence: 99%

Mitogen-activated protein kinase phosphatase (MKP)-1 in immunology, physiology, and disease

2012

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Mitogen-activated protein kinases (MAPKs) are key regulators of cellular physiology and immune responses and abnormality in MAPKs is implicated in many diseases. MAPKs are activated by MAPK kinases through phosphorylation of the threonine and tyrosine residues in the conserved Thr-Xaa-Tyr domain, where Xaa represents amino acid residues characteristic of distinct MAPK subfamilies. Since MAPKs play a crucial role in a variety of cellular processes, a delicate regulatory network has evolved to control their activities. Over the past two decades, a group of dual specificity MAPK phosphatases (MKPs) have been identified that deactivate MAPKs. Since MAPKs can enhance MKP activities, MKPs are considered as an important feedback control mechanism that limits the MAPK cascades. This review outlines the role of MKP-1, a prototypical MKP family member, in physiology and disease. We will first discuss the basic biochemistry and regulation of MKP-1. Next, we will present the current consensus on the immunological and physiological functions of MKP-1 in infectious, inflammatory, metabolic, and nervous system diseases as revealed by studies using animal models. We will also discuss the emerging evidence implicating MKP-1 in human disorders. Finally, we will conclude with a discussion of the potential for pharmacomodulation of MKP-1 expression.

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“…Additionally, it is an important cofactor affecting cell proliferation, differentiation, apoptosis, angiogenesis and survival. As a tumor suppressor, BTG1 is involved in the pathogenesis of several diseases, including breast cancer, multiple sclerosis, ovarian cancer and prostate cancer [26][27][28][29][30][31][32][33] . In hepatocellular carcinoma, BTG1 expression is decreased, and it has recently been reported that BTG1 ameliorates liver steatosis by decreasing stearoyl-CoA desaturase 1 (SCD1) levels and altering hepatic lipid metabolism [34] .…”

Section: Introductionmentioning

confidence: 99%

miR-511 promotes the proliferation of human hepatoma cells by targeting the 3′UTR of B cell translocation gene 1 (BTG1) mRNA

et al. 2017

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Aberrant expression of miR-511 is involved in the development of cancer, but the role of miR-511 in hepatocellular carcinoma (HCC) is not well documented. In this study, we explored the molecular mechanisms of miR-511 in hepatocarcinogenesis. Our results of bioinformatics analysis suggested that B cell translocation gene 1 (BTG1), a member of anti-proliferative gene family, was one of the putative targets of miR-511. The expression levels of miR-511 were significantly higher in 30 clinical HCC tissues than in corresponding peritumor tissues, and were negatively correlated with those of BTG1 in the HCC tissues (r=-0.6105, P<0.01). In human hepatoma cell lines HepG2 and H7402, overexpression of miR-511 dose-dependently inhibited the expression of BTG1, whereas knockdown of miR-511 dose-dependently increased the expression of BTG1. Luciferase reporter gene assays verified that miR-511 targeted the 3'UTR of BTG1 mRNA. In the hepatoma cells, overexpression of miR-511 significantly decreased BTG1-induced G phase arrest, which was rescued by overexpression of BTG1. Furthermore, overexpression of miR-511 promoted the proliferation of the hepatoma cells, which was rescued by overexpression of BTG1. Conversely, knockdown of miR-511 inhibited cell proliferation, which was reversed by knockdown of BTG1. In conclusion, miR-511 promotes the proliferation of human hepatoma cells in vitro by targeting the 3'UTR of BTG1 mRNA.

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Identification and allelic frequencies of novel single-nucleotide polymorphisms in the DUSP1 and BTG1 genes

Cited by 9 publications

References 5 publications

Salivary Transcriptome Diagnostics for Oral Cancer Detection

Salivary Transcriptome Diagnostics for Oral Cancer Detection

Mitogen-activated protein kinase phosphatase (MKP)-1 in immunology, physiology, and disease

miR-511 promotes the proliferation of human hepatoma cells by targeting the 3′UTR of B cell translocation gene 1 (BTG1) mRNA

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