Identification and Anti‐oxidant Capacity Determination of Phenolics and their Glycosides in Elderflower by On‐line HPLC–CUPRAC Method

Çelik, Saliha Esin; Özyürek, Mustafa; Güçlü, Kubilay; Çapanoğlu, Esra; Apak, Reşat

doi:10.1002/pca.2481

Cited by 35 publications

(10 citation statements)

References 26 publications

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“…Elderberries are highly enriched in anthocyanins (Milbury et al, 2002; Veberic et al, 2009), and anthocyanin-rich extracts prepared from elderberries or other botanicals, in addition to individual anthocyanins, exhibit an array of antioxidant activities (Netzel et al, 2005; Strathearn et al, 2014; Zafra-Stone et al, 2007). The flower portion of the elderberry plant (referred to here as ‘elderflower’) also contains high levels of polyphenolic constituents, including quercetin-3-O-rutinoside, the most abundant component in the methanolic extract (Celik et al, 2014; Rieger et al, 2008). Analyses of elderflower extracts in cell culture models revealed neuroprotective, anti-inflammatory, and antioxidant activities (Barros et al, 2011; Harokopakis et al, 2006) (see accompanying article, de Rus Jacquet et al, submitted).…”

Section: Resultsmentioning

confidence: 99%

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

Jacquet

Tambe

Ying

et al. 2017

Journal of Ethnopharmacology

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Ethnopharmacological relevance Parkinson’s disease (PD) is a multifactorial neurodegenerative disorder affecting 5% of the population over the age of 85 years. Current treatments primarily involve dopamine replacement therapy, which leads to temporary relief of motor symptoms but fails to slow the underlying neurodegeneration. Thus, there is a need for safe PD therapies with neuroprotective activity. In this study, we analyzed contemporary herbal medicinal practices used by members of the Pikuni-Blackfeet tribe from Western Montana to treat PD-related symptoms, in an effort to identify medicinal plants that are affordable to traditional communities and accessible to larger populations. Aim of the study The aims of this study were to (i) identify medicinal plants used by the Pikuni-Blackfeet tribe to treat individuals with symptoms related to PD or other CNS disorders, and (ii) characterize a subset of the identified plants in terms of antioxidant and neuroprotective activities in cellular models of PD. Materials and Methods Interviews of healers and local people were carried out on the Blackfeet Indian reservation. Plant samples were collected, and water extracts were produced for subsequent analysis. A subset of botanical extracts was tested for the ability to induce activation of the Nrf2-mediated transcriptional response and to protect against neurotoxicity elicited by the PD-related toxins rotenone and paraquat. Results The ethnopharmacological interviews resulted in the documentation of 19 medicinal plants used to treat various ailments and diseases, including symptoms related to PD. Seven botanical extracts (out of a total of 10 extracts tested) showed activation of Nrf2-mediated transcriptional activity in primary cortical astrocytes. Extracts prepared from Allium sativum cloves, Trifolium pratense flowers, and Amelanchier arborea berries exhibited neuroprotective activity against toxicity elicited by rotenone, whereas only the extracts prepared from Allium sativum and Amelanchier arborea alleviated PQ-induced dopaminergic cell death. Conclusions Our findings highlight the potential clinical utility of plants used for medicinal purposes over generations by the Pikuni-Blackfeet people, and they shed light on mechanisms by which the plant extracts could slow neurodegeneration in PD.

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Section: Resultsmentioning

confidence: 99%

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

Jacquet

Tambe

Ying

et al. 2017

Journal of Ethnopharmacology

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“…For neochlorogenic acid, chlorogenic acid, rutin, isoquercitrin and narcissin, corresponding content ranges were shown to be 1.06-1.60, 12.40-14.00, 15.70-23.90, 0.73-3.05, and 4.26-5.33 mg g −1 dm [42]. Data from another study on EBF extracts were in line, reporting contents for chlorogenic acid, rutin, isoquercitrin, and quercetin of 5.93, 15.28, 2.64, and 0.11 mg g −1 dm [43]. Rutin and isoquercitrin contents of 20.2 and 0.97 mg g -1 have also been reported, in EBF extracts obtained with pressurized liquid extraction [44].…”

Section: Polyphenolic Compositionmentioning

confidence: 93%

A Green Extraction Process for Polyphenols from Elderberry (Sambucus nigra) Flowers Using Deep Eutectic Solvent and Ultrasound-Assisted Pretreatment

et al. 2020

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Sambucus nigra flowers, known as elderberry flowers (EBF), are a plant tissue rich in polyphenolic phytochemicals with important bioactivities. However, there are few studies dealing with the production of polyphenol-containing EBF extracts. The objective of the investigation presented herein was the development of a high-performance green extraction methodology, to generate EBF extracts enriched in polyphenolic substances, using an efficient deep eutectic solvent, combined with ultrasonication pretreatment. The DES was composed of L-lactic acid (hydrogen bond donor—HBD) and glycine (hydrogen bond acceptor—HBA) and, after an initial screening to properly regulate HBD/HBA ratio, the extraction was optimized by deploying response surface methodology. Under the optimized conditions, which were DES/water (85% w/v), liquid-to-solid ratio 60 mL g−1, and stirring speed 200 rounds per minute, the extraction yield in total polyphenols amounted to 121.24 ± 8.77 mg gallic acid equivalents per g dry matter. The integration of ultrasonication prior to the batch stirred-tank extraction boosted polyphenol recovery of up to 174.73 ± 2.62 mg gallic acid equivalents per g dry matter. Liquid chromatography–mass spectrometry analysis showed that the richest EBF extract obtained was dominated by rutin, a di-p-coumaroylquic acid and chlorogenic acid.

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“…According to the previous studies, the fluctuation of the pump pressure and flow rate was the primary source of noise in the assay (Damašius et al, 2014;Esin Ç elik et al, 2014;Murkovic, 2002;Sun et al, 2012;Yan et al, 2014;Zhang et al, 2014). To reduce the noise, a non-return valve was added to the system which decreases the fluctuations in pump pressure and prevents the solution reverse.…”

Section: Application Of Non-return Valvementioning

confidence: 99%

“…Optimization of the system DPPH concentration, DPPH velocity and reaction tank length are all factors that influence the estimated radical scavenging capacity of the sample (Damašius et al, 2014;Yan et al, 2014;Zhang et al, 2014). Rutin, a model antioxidant, was used to optimize the DPPH concentration, DPPH velocity and reaction tank length; the signal to noise patio (S/N) of the radical was used as the evaluation standard (Esin Ç elik et al, 2014;Zhang et al, 2014). Response surface methodology (RSM) was used to determine the best combination of experimental conditions for the on-line HPLC-DPPH assay.…”

Section: Hplc-esi-ms/ms Analysismentioning

confidence: 99%

Antioxidants screening in Limonium aureum by optimized on-line HPLC–DPPH assay

Dong

Chi

Qian

et al. 2015

Industrial Crops and Products

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Identification and Anti‐oxidant Capacity Determination of Phenolics and their Glycosides in Elderflower by On‐line HPLC–CUPRAC Method

Abstract: The on-line HPLC-CUPRAC method was advantageous over on-line ABTS and DPPH methods for measuring the flavonoid glycosides of elderflower.

Cited by 35 publications

References 26 publications

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

Pikuni-Blackfeet traditional medicine: Neuroprotective activities of medicinal plants used to treat Parkinson’s disease-related symptoms

A Green Extraction Process for Polyphenols from Elderberry (Sambucus nigra) Flowers Using Deep Eutectic Solvent and Ultrasound-Assisted Pretreatment

Antioxidants screening in Limonium aureum by optimized on-line HPLC–DPPH assay

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