Identification and Antibiogram of Various Gram Positive Bacterial Isolates from Pyogenic Samples by VITEK® 2 Compact System

Khullar, Shivani; Rathore, Laxmi; Khatri, P. K.; Parihar, R. S.; Meena, Saroj; Bora, Archana; Maurya, Vinod Kumar; Sharma, Niranjan

doi:10.20546/ijcmas.2016.502.007

Cited by 5 publications

(2 citation statements)

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“…This was in contrast to other studies [9,14] that shown S. aureus which is the most common isolate among GP in ascitic fluid. Our study is in accordance to study by Shivani et al [15] that reported Enterococcus as the most common GP isolates in peritoneal fluid. Ding et al [16] also shown that Enterococcus spp.…”

Section: Discussionsupporting

confidence: 93%

A Culture-antibiogram of Sterile Body Fluids in a Tertiary Care Hospital at Central Delhi in Emergency Settings

Suman

Lal

Chaudhary

et al. 2022

APJHS

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Infection of sterile body fluids could be life-threatening. Monitoring of prevailing infectious agents and their resistance pattern through antibiogram helps in appropriate selection of antibiotics and strengthens antibiotic stewardship. The aim of this study is to isolate infective bacteria from sterile body fluids and to determine antibiotic resistant pattern of these isolates. This retrospective observational study was conducted through January 2018–December 2018 in a tertiary care hospital at central Delhi. Sterile body fluids were processed as per the standard laboratory procedures. Antibiotic susceptibility pattern was determined by Kirby Bauer’s disk diffusion method and interpreted as per Clinical and Laboratory Standards Institute guidelines. A total of 3,703 fluid samples were studied. Out of them 49.9% were ascitic fluid, 39.4% were pleural fluid, and 10.7% were others such as pericardial fluid, synovial fluid, bile, peritoneal dialysis fluid, and bronchoalveolar lavage fluid. Bacterial isolation rate was 12.93%. Predominant organisms were Escherichia coli (31%), Acinetobacter spp. (20%), Klebsiella spp. (16%), Staphylococcus aureus (11%), and Enterococcus spp. (10%). Isolation of vancomycin resistant enterococcus was 9.5% and Methicillin resistant S. aureus was 15.6%. Gram-negative isolates were 100% sensitive to colistin, followed by imipenem and gentamicin. Increased resistance shown toward third generation cephalosporins, amoxicillin + clavulinic acid, and piperacillin + tazobactum. Klebsiella spp. was the most resistant isolate, while Enterococcus spp. was the most resistant among gram positive isolates. Changing antimicrobial pattern poses challenge in treating infective agents. This culture antibiogram helps in appropriate selection of antibiotics in our setting and eventually decrease antibiotic resistant and patient’s morbidity and mortality.

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Section: Discussionsupporting

confidence: 93%

A Culture-antibiogram of Sterile Body Fluids in a Tertiary Care Hospital at Central Delhi in Emergency Settings

Suman

Lal

Chaudhary

et al. 2022

APJHS

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“…The susceptibility of the bacterial isolates to different antimicrobials was determined through the VITEK system, for which three milliliters of sterile saline solution was transferred to a test tube, later an isolated colony was selected, mixed and the density determined through the McFarland scale, which should have been between 0.5 -0.63 (16,17) . In the susceptibility studies, the GNS 113 and GPS 102 cards were used for Gram-negative bacilli and Gram-positive cocci, respectively (16) .…”

Section: Susceptibility Testingmentioning

confidence: 99%

Resistance profiles of bacterial pathogens isolated in a hospital institution in Montería - Córdoba, 2018

lez¹,

n²,

Cornejo³

et al. 2020

IJST

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Objective: To determine the resistance profiles of bacterial pathogens isolated in a hospital institution in Montería -Córdoba. Methodology: An observational study was carried out, during the months of January to December 2018; 344 samples were evaluated. Mention about the source of study samples. The identification and susceptibility of the bacteria were determined through the VITEK system. Findings: Among the Gram-negative bacteria identified were Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii and Serratia marcescens, while Gram-positives were the Staphylococcus epidermidis, S. aureus, S. haemolyticus and Enterococcus faecalis. Gram-negative isolates such as Klebsiella pneumoniae showed resistance to piperacilinatazobactam, ceftazidima, cefepima, doripenem, imipenem, meropenem, amikacina and ciprofloxacina, being sensitive to gentamicin. P. aeruginosa, E. coli and A. baumannii showed resistance to all antimicrobials, with the exception of colistin. S. marcescens only showed resistance to ceftazidime. Enterobacter cloacae showed resistance to most drugs, with the exception of amikacin and gentamicin. Among the Gram-positive isolates, S. epidermidis showed resistance to Trimethoprim / Sulfamethoxazole, erythromycin, nitrofurantoin, ciprofloxacin, and levofloxacin. S. aureus and Staphylococcus haemolyticus showed susceptibility to all antimicrobials, while E. faecalis and Staphylococcus hominis only showed resistance to erythromycin. Conclusion: A wide prevalence of antimicrobial resistance (mention the milieu) was observed in this investigation.

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Study of the Genomic Characterization of Antibiotic-Resistant Escherichia Coli Isolated From Iraqi Patients with Urinary Tract Infections

Mouhammed,

Gdoura

2023

Indian J Microbiol

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Identification and Antibiogram of Various Gram Positive Bacterial Isolates from Pyogenic Samples by VITEK® 2 Compact System

Cited by 5 publications

References 0 publications

A Culture-antibiogram of Sterile Body Fluids in a Tertiary Care Hospital at Central Delhi in Emergency Settings

A Culture-antibiogram of Sterile Body Fluids in a Tertiary Care Hospital at Central Delhi in Emergency Settings

Resistance profiles of bacterial pathogens isolated in a hospital institution in Montería - Córdoba, 2018

Study of the Genomic Characterization of Antibiotic-Resistant Escherichia Coli Isolated From Iraqi Patients with Urinary Tract Infections

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