Identification and Antibiotic‐Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends

Maugeri, Gaetano; Lychko, Iana; Sobral, Rita G.; Roque, Ana C. A.

doi:10.1002/biot.201700750

Cited by 131 publications

(103 citation statements)

References 149 publications

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“…Other classes of antibiotic such as polymyxins (Colistin) cause biophysical changes in cell membrane permeability prior to cell death. Consequently, AST methods that rely on cell growth or metabolic activity do not report results in <6 h 12 , 13 . To address this, several rapid AST assays have been developed 13 – 15 .…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, AST methods that rely on cell growth or metabolic activity do not report results in <6 h 12 , 13 . To address this, several rapid AST assays have been developed 13 – 15 . Flow cytometry has been widely proposed for rapid ASTs: antibiotic exposure leads to changes in susceptible strains of bacteria that can be measured by (label-free) light scatter and/or fluorescent viability markers 16 .…”

Section: Introductionmentioning

confidence: 99%

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A fast impedance-based antimicrobial susceptibility test

et al. 2020

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There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 105 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A fast impedance-based antimicrobial susceptibility test

et al. 2020

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“…There are two other major chapters in infectious disease relevant for this review, apart from pure diagnosis: antibiotic susceptibility testing [153,154] and sepsis early diagnosis [155][156][157][158]. Table 2.…”

Section: Medical Clinical Applicationsmentioning

confidence: 99%

Emerging Designs of Electronic Devices in Biomedicine

et al. 2020

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A long-standing goal of nanoelectronics is the development of integrated systems to be used in medicine as sensor, therapeutic, or theranostic devices. In this review, we examine the phenomena of transport and the interaction between electro-active charges and the material at the nanoscale. We then demonstrate how these mechanisms can be exploited to design and fabricate devices for applications in biomedicine and bioengineering. Specifically, we present and discuss electrochemical devices based on the interaction between ions and conductive polymers, such as organic electrochemical transistors (OFETs), electrolyte gated field-effect transistors (FETs), fin field-effect transistor (FinFETs), tunnelling field-effect transistors (TFETs), electrochemical lab-on-chips (LOCs). For these systems, we comment on their use in medicine.

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“…For this purpose, microbiological methods are used, i.e., plating onto a culture medium [5]. After confirming the presence of bacterial growth, the sensitivity of the detected pathogen to the main classes of antibiotic drugs is determined [6], and the antibiotics that best penetrate the affected tissues are selected.…”

Section: Use Of Antibiotic Drugs In Treatment Of Bacterial Infectionsmentioning

confidence: 99%

Monitoring Antibiotics and Inflammatory Markers in Human Blood: Impact in Choice of Antibiotic Therapy and Used Methods

Berlina¹,

Zherdev²,

Dzantiev³

2019

Preprint

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In the modern world, the problem of antibiotic therapy is acute. Despite the diversity of existing antibiotic drugs, their efficacy decreases as new, resistant forms of pathogenic microorganisms emerge. It is extremely difficult to control such processes and even more difficult to treat severe bacterial infections. In such situations, an individual approach to each patient is required and physicians need parameters to estimate the efficacy of antibiotic therapy. This review discusses the significance of monitoring the content of antibiotics in the blood for this purpose, in combination with the content of inflammatory markers, such as C-reactive protein and procalcitonin. The basic principles of antibiotic therapy, and factors in the resistance of microorganisms to antibiotics, are examined. Approaches to assess the efficacy of antibiotic therapy, as well as methods to detect antibiotics and inflammatory markers in the blood of patients, and comparative assessment of their capabilities and limitations, are described.

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Identification and Antibiotic‐Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends

Cited by 131 publications

References 149 publications

A fast impedance-based antimicrobial susceptibility test

A fast impedance-based antimicrobial susceptibility test

Emerging Designs of Electronic Devices in Biomedicine

Monitoring Antibiotics and Inflammatory Markers in Human Blood: Impact in Choice of Antibiotic Therapy and Used Methods

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