Identification and application of Streptomyces microflavus G33 in compost to suppress tomato bacterial wilt disease

Shen, Tong; Lei, Yunhui; Pu, Xiaodong; Zhang, Siyang; Du, Yuhui

doi:10.1016/j.apsoil.2020.103724

Cited by 32 publications

(12 citation statements)

References 61 publications

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“…In this study, we successfully isolated and screened a strain R31 for potential use as an effective biocontrol agent against TBW and showed that it effectively suppressed this disease in pot experiment. While many species have been used for biological control of TBW, including Streptomyces (Ling et al, 2020), Bacillus methylotrophicus (Im et al, 2020), Bacillus amyloliquefaciens (Wei et al, 2011;Ho et al, 2020), Bacillus velezensis (Chen et al, 2020), Streptomyces microflavus (Shen et al, 2021), information on the use of myxobacteria as biocontrol agents in controlling TBW is blank. In fact, myxobacteria have great potential in biocontrol of plant diseases.…”

Section: Discussionmentioning

confidence: 99%

Myxococcus xanthus R31 Suppresses Tomato Bacterial Wilt by Inhibiting the Pathogen Ralstonia solanacearum With Secreted Proteins

Dong

Gao

et al. 2022

Front. Microbiol.

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The pathogenic bacterium Ralstonia solanacearum caused tomato bacterial wilt (TBW), a destructive soil-borne disease worldwide. There is an urgent need to develop effective control methods. Myxobacteria are microbial predators and are widely distributed in the soil. Compared with other biocontrol bacteria that produce antibacterial substances, the myxobacteria have great potential for biocontrol. This study reports a strain of Myxococcus xanthus R31 that exhibits high antagonistic activity to R. solanacearum. Plate test indicated that the strain R31 efficiently predated R. solanacearum. Pot experiments showed that the biocontrol efficacy of strain R31 against TBW was 81.9%. Further study found that the secreted protein precipitated by ammonium sulfate had significant lytic activity against R. solanacearum cells, whereas the ethyl acetate extract of strain R31 had no inhibitory activity against R. solanacearum. Substrate spectroscopy assay and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of secreted proteins showed that some peptidases, lipases, and glycoside hydrolases might play important roles and could be potential biocontrol factors involved in predation. The present study reveals for the first time that the use of strain M. xanthus R31 as a potential biocontrol agent could efficiently control TBW by predation and secreting extracellular lyase proteins.

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Section: Discussionmentioning

confidence: 99%

Myxococcus xanthus R31 Suppresses Tomato Bacterial Wilt by Inhibiting the Pathogen Ralstonia solanacearum With Secreted Proteins

Dong

Gao

et al. 2022

Front. Microbiol.

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“…Nocardiopsis species exhibit antifungal activity against Fusarium species and produce various antimicrobial metabolites (Intra et al, 2011). The antagonistic activity of actinomycetes against plant pathogens depends on their ability to produce hydrolytic enzymes, antifungal metabolites, competition for nutrients, siderophores, among others (Igarashi et al, 2021;Shen et al, 2021;Vurukonda et al, 2018).…”

Section: In Vitro Antagonism Tests Of Marine Actinomycetes Against F ...mentioning

confidence: 99%

“…The use ofactinomycetes as biocontrol agents towards phytopathogens is an alternative to the application of agrochemicals (Wang et al, 2018). Among the antagonistic mechanisms of actinomycetes are, antibiotics, siderophores, induction of host resistance, hydrolytic enzymes, among others (Gopalakrishnan et al, 2021;Igarashi et al, 2021;Shen et al, 2021). Actinomycetes from terrestrial environments have been extensively studied, however, actinomycetes from marine environments are an understudied resource, which may be more efficient in controlling plant phytopathogens (Gong et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification and morphological characterization of marine actinomycetes as biocontrol agents of Fusarium solani in tomato

Torres-Rodríguez¹,

Pérez

Castellanos³

et al. 2022

RevFacAgron(LUZ)

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Fusarium spp. damages the roots of crops, its control is with synthetic fungicides, however, marine actinomycetes can be an option to the use of agrochemicals. The objective of this work was the identification and morphological characterization of marine actinomycetes as antagonists to Fusarium solani (Mart.) Sacc. Fusarium spp. was isolated from diseased tomato plants and mangrove sediment actinomycetes, both identified through taxonomic keys and molecular techniques. Eight isolates of Fusarium spp. were obtained, H8 being the most virulent and it was identified as F. solani. Thirty actinomycetes were isolated, of which only four inhibited the phytopathogen, being A19 the one that inhibited the fungus by 70% and was identified as Streptomyces sp. Marine actinomycetes may be an option for disease management in plants of agricultural interest.

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“…[3][4][5] The use of biological control agents (BCAs) is increasing worldwide and is a promising alternative for controlling soil-borne diseases in sustainable and organic agriculture. 6,7 Avirulent strains of RS, Bacillus spp., and Streptomyces spp. are well-known BCAs.…”

Section: Introductionmentioning

confidence: 99%

An avirulent Ralstonia solanacearum strain FJAT1458 outcompetes with virulent strain and induces tomato plant resistance against bacterial wilt

Zheng

Xiao

Chen

et al. 2022

Pest Management Science

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BACKGROUND Bacterial wilt (BW) caused by Ralstonia solanacearum (RS) is considered as one of the most destructive plant diseases. An avirulent strain of RS, FJAT1458, is a potential biocontrol agent of BW. In this study, the mechanism of FJAT1458 against BW was evaluated. RESULTS FJAT1458 was tagged with the red fluorescent protein gene, and the resulting strain was named as FJAT1458‐RFP. When FJAT1458‐RFP and FJAT91‐GFP (a virulent strain of RS labelled with the green fluorescent protein gene), were co‐inoculated in potted tomato plants, the colonization of FJAT91‐GFP reached an almost undetectable level at 7 days post‐inoculation (dpi) in the roots and at 9 dpi in rhizosphere soil. When they were co‐inoculated in a hydroponic tomato growing system, numbers of the two strains were similar at 3 dpi in the root tissues; however, FJAT91‐GFP was not detected at 9 dpi while FJAT1458‐RFP maintained 1.77 × 105 CFU g−1. The inoculation of FJAT1458‐RFP alone or combination with FJAT91‐GFP significantly increased tomato root activity. Moreover, expression levels of the defense‐related genes PR‐1a, GLUA, and CHI3 in tomato roots were significantly up‐regulated by FJAT1458‐RFP and co‐inoculation of FJAT1458‐RFP and FJAT91‐GFP at 5 dpi, compared to the control (water, CK) treatment. Noteworthy, expression levels of GLUA in the treatments of FJAT1458‐RFP and FJAT1458‐RFP + FJAT91‐GFP were 12.22‐ and 12.05‐fold higher than that in the CK at 5 dpi, respectively. CONCLUSIONS The results suggested that the avirulent strain FJAT1458‐RFP could suppress colonization of the virulent strain in tomato roots, and induce tomato plant resistance against BW. © 2022 Society of Chemical Industry.

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Identification and application of Streptomyces microflavus G33 in compost to suppress tomato bacterial wilt disease

Cited by 32 publications

References 61 publications

Myxococcus xanthus R31 Suppresses Tomato Bacterial Wilt by Inhibiting the Pathogen Ralstonia solanacearum With Secreted Proteins

Myxococcus xanthus R31 Suppresses Tomato Bacterial Wilt by Inhibiting the Pathogen Ralstonia solanacearum With Secreted Proteins

Identification and morphological characterization of marine actinomycetes as biocontrol agents of Fusarium solani in tomato

An avirulent Ralstonia solanacearum strain FJAT1458 outcompetes with virulent strain and induces tomato plant resistance against bacterial wilt

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