Identification and applications of neuroactive silk proteins: a narrative review

Mohammadi, Ahad Banagozar; Sadigh‐Eteghad, Saeed; Torbati, Mohammadali; Fazljou, Seyyed Mohammad Bagher; Vatandoust, Seyed Mehdi; Golzari, Samad Ej; Farajdokht, Fereshteh; Mahmoudi, Javad

doi:10.32725/jab.2019.012

Cited by 18 publications

(18 citation statements)

References 116 publications

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“…It was reported that BF-7 treatment after MCAO significantly decreased infarct volume in the rat brain [ 21 ]. To the best of our knowledge, neuroprotective effects in other cocoon hydrolysate were not investigated, but only in BF-7 [ 38 ]. In this study, we firstly demonstrated the neuroprotective effect of BF-7 against transient forebrain ischemia in gerbils.…”

Section: Discussionmentioning

confidence: 99%

Brain Factor-7® improves learning and memory deficits and attenuates ischemic brain damage by reduction of ROS generation in stroke in vivo and in vitro

et al. 2020

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Brain Factor-7® (BF-7), silk fibroin peptide, is known to be effective in improvement of memory and learning ability. In this study, the effects of BF-7 (10 mg/kg, p.o., pre-treatment for 7 days and post-treatment for 7 days) on neuroprotection and memory and learning functions were investigated in a rat model of transient focal cerebral ischemia and a gerbil model of transient global forebrain ischemia. Furthermore, to find the mechanism of BF-7, we examined the neuroprotective and antioxidative effects of BF-7 in vitro using neuroblastoma (SH-SY5Y) cells. In vivo model, treatment with BF-7 significantly reduced the number of errors in 8-arm maze test and significantly increased latency time in passive avoidance test at 7 days after focal ischemia compared to those in the vehicle-treated group. In addition, treatment with BF-7 significantly decreased the infarct size or neuronal death at 7 day following transient ischemia compared to that in the vehicle-treated group. In vitro model, 10 or 20 μg/ml of BF-7 treatment significantly increased cell viability in dose-dependent manner. In addition, oxidative stress was significantly attenuated in the ischemic cells, showing that 10 or 20 μg/ml of BF-7 treatment significantly reduced the generation of reactive oxygen species (ROS) compared to that in the ischemic cells. These results indicate that BF-7 treatment can attenuate ischemic damages and improve memory deficits via reduction of ROS generation.

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Section: Discussionmentioning

confidence: 99%

Brain Factor-7® improves learning and memory deficits and attenuates ischemic brain damage by reduction of ROS generation in stroke in vivo and in vitro

et al. 2020

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“…Additionally, it has been found that PD is followed by the production of tyrosinases that are involved in neurodegeneration [ 136 ]. In PD, overexpression of tyrosinases causes hydroxylation, which results in a reduction in dopamine content and the production of ROS, which both cause neuronal cell death [ 136 , 137 ].…”

Section: Silk Fibroin In the Context Of Neurodegenerative Diseasesmentioning

confidence: 99%

“…SS has been found as an inhibitor of tyrosinases [ 138 ], while the peptides of SF have shown neuroprotection against 6-OHDA-induced neurodegeneration [ 137 ]. SF-derived peptides preserved the viability of dopaminergic neurons in response to 6-hydroxydopamine neurotoxicity in a PD animal model induced by 6-OHDA [ 130 ].…”

Section: Silk Fibroin In the Context Of Neurodegenerative Diseasesmentioning

confidence: 99%

“…SF-derived peptides preserved the viability of dopaminergic neurons in response to 6-hydroxydopamine neurotoxicity in a PD animal model induced by 6-OHDA [ 130 ]. Alternatively, it has been reported that SF peptides show inhibitory activity against monoamine oxidases (MAOs), important enzymes that cause the breakage of monoamines such as L-DOPA [ 137 ]. Extensive activity of silkworm extracts against MAO-A and MAO-B, in areas of the substantia nigra and cerebral cortex, was reported [ 47 ], and this concept was extended in a second work, where the injection of silk extracts antagonized the effects of N -methyl-4-phenyl-1,2,3,6-tetrahydropyridine, a precursor of the neurotoxin 1-methyl-4-phenylpyridinium, which is involved in the destruction of dopaminergic receptors within the substantia nigra [ 46 ].…”

Section: Silk Fibroin In the Context Of Neurodegenerative Diseasesmentioning

confidence: 99%

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Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

et al. 2021

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Silk refers to a family of natural fibers spun by several species of invertebrates such as spiders and silkworms. In particular, silkworm silk, the silk spun by Bombyx mori larvae, has been primarily used in the textile industry and in clinical settings as a main component of sutures for tissue repairing and wound ligation. The biocompatibility, remarkable mechanical performance, controllable degradation, and the possibility of producing silk-based materials in several formats, have laid the basic principles that have triggered and extended the use of this material in regenerative medicine. The field of neural soft tissue engineering is not an exception, as it has taken advantage of the properties of silk to promote neuronal growth and nerve guidance. In addition, silk has notable intrinsic properties and the by-products derived from its degradation show anti-inflammatory and antioxidant properties. Finally, this material can be employed for the controlled release of factors and drugs, as well as for the encapsulation and implantation of exogenous stem and progenitor cells with therapeutic capacity. In this article, we review the state of the art on manufacturing methodologies and properties of fiber-based and non-fiber-based formats, as well as the application of silk-based biomaterials to neuroprotect and regenerate the damaged nervous system. We review previous studies that strategically have used silk to enhance therapeutics dealing with highly prevalent central and peripheral disorders such as stroke, Alzheimer’s disease, Parkinson’s disease, and peripheral trauma. Finally, we discuss previous research focused on the modification of this biomaterial, through biofunctionalization techniques and/or the creation of novel composite formulations, that aim to transform silk, beyond its natural performance, into more efficient silk-based-polymers towards the clinical arena of neuroprotection and regeneration in nervous system diseases.

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“…SP is a bioactive material that is extracted from the silkworm Bombyx mori and has a potential value in traditional medicine and as a dietary supplement for a broad range of health-related purposes [47]. Recent research has shown that SP has anti-diabetic, anti-tumor, anti-oxidant, anti-bacterial, cell proliferation, and wound healing effects [31,[48][49][50][51].…”

Section: Discussionmentioning

confidence: 99%

Effect of Dietary Silk Peptide on Obesity, Hyperglycemia, and Skeletal Muscle Regeneration in High-Fat Diet-Fed Mice

Lee

Jin

Chei

et al. 2020

Cells

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Obesity is associated with excess body fat accumulation that can cause hyperglycemia and reduce skeletal muscle function and strength, which characterize the development of sarcopenic obesity. In this study, we aimed to determine the mechanism whereby acid-hydrolyzed silk peptide (SP) prevents high-fat diet (HFD)-induced obesity and whether it regulates glucose uptake and muscle differentiation using in vivo and in vitro approaches. Our findings demonstrate that SP inhibits body mass gain and the expression of adipogenic transcription factors in visceral adipose tissue (VAT). SP also had an anti-diabetic effect in VAT and skeletal muscle because it upregulated glucose transporter type 4 (GLUT4) and uncoupling protein 3 (UCP3) expression. Furthermore, SP reduced ubiquitin proteasome and promoted myoblast determination protein 1 (MyoD)/myogenic factor 4 (myogenin) expression, implying that it may have potential for the treatment of obesity-induced hyperglycemia and obesity-associated sarcopenia.

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Identification and applications of neuroactive silk proteins: a narrative review

Cited by 18 publications

References 116 publications

Brain Factor-7® improves learning and memory deficits and attenuates ischemic brain damage by reduction of ROS generation in stroke in vivo and in vitro

Brain Factor-7® improves learning and memory deficits and attenuates ischemic brain damage by reduction of ROS generation in stroke in vivo and in vitro

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

Effect of Dietary Silk Peptide on Obesity, Hyperglycemia, and Skeletal Muscle Regeneration in High-Fat Diet-Fed Mice

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