Identification and bioinformatic analysis of the CaCesA/Csls family members and the expression of the CaCslD1 in the flower buds of CMS/Rf system in pepper

Duan, Panpan; Wei, Min; Zhang, Ru; Zhao, Shufang; Wang, Yongfu; Gou, Bingdiao; Yang, Nan; Zhang, Tao; Zhang, Gaoyuan; Wei, Bingqiang

doi:10.1007/s10142-022-00896-y

Cited by 3 publications

(1 citation statement)

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“…In our study, we found that the distribution of RF2 genes on each chromosome was uneven, but we found that most of the number and location of genes on each chromosome of the two tetraploid cotton species were one to one, this phenomena was also observed for other transcription factors in other plant species [43][44][45][46][47][48]. This may be due to the fact that allotetraploids share the common ancestors A0 (the possible extinct common ancestor of the existing A genome) and D5 (G. raimondii) [49].…”

Section: Discussionsupporting

confidence: 58%

Genome-Wide Identification and Functional Analysis of RF2 Gene Family and the Critical Role of GhRF2-32 in Response to Drought Stress in Cotton

Zhao

Wei

et al. 2023

Plants

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Cotton is an important natural fiber crop. The RF2 gene family is a member of the bZIP transcription factor superfamily, which plays an important role in plant resistance to environmental stresses. In this paper, the RF2 gene family of four cotton species was analyzed genome-wide, and the key gene RF2-32 was cloned for functional verification. A total of 113 RF2 genes were identified in the four cotton species, and the RF2 family was relatively conserved during the evolution of cotton. Chromosome mapping and collinear analysis indicated that fragment replication was the main expansion mode of RF2 gene family during evolution. Cis-element analysis showed that there were many elements related to light response, hormone response and abiotic stress response in the promoters of RF2 genes. The transcriptome and qRT-PCR analysis of RF2 family genes in upland cotton showed that RF2 family genes responded to salt stress and drought stress. GhRF2-32 protein was localized in the cell nucleus. Silencing the GhRF2-32 gene showed less leaf wilting and increased total antioxidant capacity under drought and salt stress, decreased malondialdehyde content and increased drought and salt tolerance. This study revealed the evolutionary and functional diversity of the RF2 gene family, which laid a foundation for the further study of stress-resistant genes in cotton.

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