Identification and characterisation of main allergic proteins in<i>Vitis vinifera vitis</i>

Falak, Reza; Sankian, Mojtaba; Noorbakhsh, Reihaneh; Tehrani, Mohsen; Assarehzadegan, Mohammad-Ali; Azad, Farahzad Jabbari; Abolhasani, Ahmad; Varasteh, Abdolreza

doi:10.1080/09540105.2012.683167

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…Since there was no report about wolf-herring parvalbumin characteristics, we first purified the native parvalbumin and predicted its sequence by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as previously reported (Falak et al, 2013). Based on deduction from computed results, the fragments derived from enzymatic digestion of wolf-herring parvalbumin were highly similar to gold fish (Carassius auratus) parvalbumin (Accession No.…”

Section: Cloning and Expression Of Parvalbumin In Prokaryotic Systemmentioning

confidence: 87%

“…Proteins were resolved on an AKTA Prime Plus FPLC system (GE healthcare) using a diethylaminoethyl (DEAE) sepharose CL-6B column as the anion exchange matrix and eluted at a flow rate of 1 ml/min by a linear gradient mixture of starting buffer (20 mM Tris-HCl pH 8.6) supplemented with 1 M NaCl. Following SDS-PAGE, the desired fractions were selected for further studies (Falak et al, 2013;Falak, Varasteh, Ketabdar, & Sankian, 2014).…”

Section: Parvalbumin Purificationmentioning

confidence: 99%

“…Proteins were separated by reducing SDS-PAGE as previously described (Falak et al, 2013). In brief, the samples were subjected to 15% SDS-PAGE, and the gels were stained with Coomassie Brilliant Blue G-250 or alternatively, proteins were electrotransferred and immunoblotted.…”

Section: Sds-page and Western Blottingmentioning

confidence: 99%

“…For Western blotting, the proteins were transferred on PVDF membranes (Amersham Biosciences, Uppsala, Sweden) using a semidry transfer system as previously described (Falak et al, 2013). The membranes were cut into strips and blocked with 2% BSA for 4 h at 37°C.…”

Section: Sds-page and Western Blottingmentioning

confidence: 99%

“…After another wash, the membranes were incubated with 1:30000 diluted horseradish peroxidase (HRP)-conjugated streptavidin (Sigma, USA) for 1 h at RT. The reactive bands were visualized with a chemiluminescent substrate and ultra-photosensitive film (Falak et al, 2013).…”

Section: Sds-page and Western Blottingmentioning

confidence: 99%

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Expression of recombinant parvalbumin from wolf-herring fish and determination of its IgE-binding capability

Mohammadi

Mokhtarian

Kardar

et al. 2017

Food and Agricultural Immunology

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In this study, we produced the recombinant form of parvalbumin from wolf-herring fish and determined its IgE reactivity. Parvalbumin cDNA was sub-cloned into pET28 and expressed in Escherichia coli BL-21. The immunoreactivities of the recombinant and native parvalbumins were compared, and the effect of calcium binding was determined by sera from 25 fish-allergic patients. ELISA and Western blotting confirmed similar IgEreactivities of the recombinant and native proteins and confirmed that this phenomenon is highly dependent on calcium binding. The recombinant protein was 94.5% similar to carp parvalbumin (Cyp c1). Approximately 72% of patients reacted strongly with recombinant parvalbumin, 80% of them reacted with the native form and only 56% showed IgE reactivity with crude extract. Because the IgE-binding capacity of recombinant wolf-herring parvalbumin is retained and is highly similar to Cyp c1, the wild and hypoallergenic forms of this allergen could be used for diagnosis and immunotherapy of fish allergy, respectively. ARTICLE HISTORY

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Section: Cloning and Expression Of Parvalbumin In Prokaryotic Systemmentioning

confidence: 87%

Section: Parvalbumin Purificationmentioning

confidence: 99%

Section: Sds-page and Western Blottingmentioning

confidence: 99%

Section: Sds-page and Western Blottingmentioning

confidence: 99%

Section: Sds-page and Western Blottingmentioning

confidence: 99%

See 3 more Smart Citations

Expression of recombinant parvalbumin from wolf-herring fish and determination of its IgE-binding capability

Mohammadi

Mokhtarian

Kardar

et al. 2017

Food and Agricultural Immunology

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Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens

et al. 2016

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In several studies, different antigenic preparations and diverse immunological tests were applied for serodiagnosis of Fasciola hepatica infections. Most of these preparations showed cross-reactivity with proteins of other parasites. Application of purified antigens might reduce these cross-reactivities. Here, we used fast protein liquid chromatography (FPLC)-fractionated extracts of F. hepatica excretory/secretory antigens (E/S Ags) for serodiagnosis of human and sheep fasciolosis. To develop an improved diagnostic method, we fractionated F. hepatica E/S Ags by anion exchange chromatography on a Sepharose CL-6B column and then tested the serodiagnostic values of the fractions. We used sera from F. hepatica-infected human and sheep as positive controls. Sera from patients with hydatidosis and strongyloidiasis were used for cross-reactivity studies. Enzyme-linked immunosorbent assays (ELISA) of the second FPLC peak, containing 20, 25, and 70 kDa proteins, discriminated between F. hepatica-infected and uninfected human and sheep samples. Fractionation of F. hepatica E/S Ags by FPLC is a fast and reproducible way of obtaining antigens useful for serodiagnosis of human and sheep fasciolosis with acceptable sensitivity and specificity. Graphical abstract ᅟ.

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Expression of grape class IV chitinase in Spodoptera frugiperda (Sf9) insect cells

Falak

Varasteh

Ketabdar

et al. 2014

Allergologia et Immunopathologia

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Identification and characterisation of main allergic proteins inVitis vinifera vitis

Cited by 8 publications

References 25 publications

Expression of recombinant parvalbumin from wolf-herring fish and determination of its IgE-binding capability

Expression of recombinant parvalbumin from wolf-herring fish and determination of its IgE-binding capability

Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens

Expression of grape class IV chitinase in Spodoptera frugiperda (Sf9) insect cells

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