Identification and characterization of a novel peritrophic matrix protein, Ae-Aper50, and the microvillar membrane protein, AEG12, from the mosquito, Aedes aegypti

Shao, Li; Devenport, Martin; Fujioka, Hisashi; Ghosh, Anil K.; Jacobs-­Lorena, Marcelo

doi:10.1016/j.ibmb.2005.03.012

Cited by 67 publications

(73 citation statements)

References 47 publications

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“…Class 3 proteins from several Lepidoptera and Diptera often contain one or more chitin-binding 'peritrophin A' domains of 60-75 amino acid residues with a conserved pattern of cysteine and aromatic residues (InterPro IPR002557). Most contain multiple chitin-binding domains which may provide cross-linking between chitin fibrils even when partially degraded by digestive proteinases (Wang et al, 2004;Shao et al, 2005). A subset of these proteins is insect intestinal mucins (IIM) which are large with highly O-glycosylated mucin domains interspersed with peritrophin domains, hence the suggestion that the PM is an analogue of vertebrate mucus reinforced by chitin.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera

Campbell

Cao

Hines

et al. 2008

Insect Biochemistry and Molecular Biology

104

107

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera

Campbell

Cao

Hines

et al. 2008

Insect Biochemistry and Molecular Biology

104

107

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“…These results suggest the chitin-binding peritrophin- (Wijffels et al, 2001;Gaines et al, 2003;Shao et al, 2005;Du et al, 2005). Expression analysis of Cb-peritrophin-15 in the Old World screwworm fly (Chrysomya bezziana) indicated it was highly tissue specific in expression.…”

Section: Mdesperi-a1 Expression During Developmentmentioning

confidence: 89%

cDNA cloning and transcriptional expression of a peritrophin‐like gene in the Hessian fly, Mayetiola destructor [Say]

Mittapalli

Sardesai

Shukle

2006

Arch Insect Biochem Physiol

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One of the well-studied components of the insect gut is the peritrophic matrix (PM). This semipermeable structure primarily functions in digestion, and protection against invasive microorganisms and mechanical damage. We report the cDNA cloning and transcription profiles of a peritrophin-A like gene (designated MdesPERI-A1) in the Hessian fly Mayetiola destructor. The predicted amino acid sequence of MdesPERI-A1 revealed a putative secretion signal peptide at its amino terminus, similarity to peritrophins from other insects including dipterans, and the presence of two chitin binding domains each containing six cysteine residues. Quantitative expression analysis of MdesPERI-A1 mRNA in different larval tissues revealed the transcript to be predominantly present in the midgut (597.9-fold) compared to other tissues assayed including salivary glands and fat bodies. Spatial expression patterns during development showed a peak expression of MdesPERI-A1 in the feeding second-instars (146-fold) and a decline in expression in the pupal and adult stages. Transcription profiling of MdesPERI-A1 during compatible (larvae on susceptible plants) and incompatible (larvae on resistant plants) interactions with wheat revealed a greater level (1.7-fold) of MdesPERI-A1 transcript in larvae on resistant plants in the initial time point examined. However, MdesPERI-A1 expression declined in larvae on resistant plants at the later time points.

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“…Proteins and chitin play important functional roles and the number of proteins in the PM varies among species (Moskalyk et al, 1996). After a blood meal, protein and chitin synthesis is activated and results in PM formation (Bertram and Bird, 1961;Staubli et al, 1966;Peters, 1992;Morlais and Severson, 2001;Shao et al, 2005). Among female adult Ae.…”

Section: Discussionmentioning

confidence: 99%

Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681)

Suwanmanee¹,

Chaisri²,

Wasinpiyamongkol

et al. 2009

Appl. Entomol. Zool.

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The peritrophic membrane (PM) is a non-cellular tissue involved in the protection of midgut epithelium from mechanical damage and insults from pathogens. This study was carried out to determine the involvement of PM in mosquitoes after infection with dengue virus. Aedes aegypti (Diptera: Culicidae) mosquitoes were fed sucrose and human blood with and without dengue virus type 2 (D2-16681), and collected at 0.5, 1, 6, and 12 h, respectively. Specimens were prepared for examination under light and electron microscopy. The results showed that PM was produced only in the blood-fed mosquitoes. The infected blood meal induced the mosquitoes to produce PM in their midgut earlier and thicker than in mosquitoes with blood alone. The initial evidence of PM occurred at 1 h post-blood meal (PBM) as a matrix-like structure. By 6 h PBM, PM had become a layer, which persisted at 12 h. Among mosquitoes fed with blood alone, this structure was found only from 6 and 12 h PBM. Dengue virus type 2 induced different modifications of mosquito PM construction and structures, confirmed under an electron microscope.

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Identification and characterization of a novel peritrophic matrix protein, Ae-Aper50, and the microvillar membrane protein, AEG12, from the mosquito, Aedes aegypti

Cited by 67 publications

References 47 publications

Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera

Proteomic analysis of the peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera

cDNA cloning and transcriptional expression of a peritrophin‐like gene in the Hessian fly, Mayetiola destructor [Say]

Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681)

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