Identification and characterization of a novel mouse and human MOPT gene containing MORN-motif protein in testis

Choi, Yun‐Jung; Hwang, Kyu‐Chan; Park, Jaein; Park, K. K.; Kim, Jin‐Hoi; Park, S. B.; Hwang, Seongsoo; Park, H.; Park, C.

doi:10.1016/j.theriogenology.2009.09.010

Cited by 12 publications

(16 citation statements)

References 18 publications

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“…MORN1 may function as a linker protein between certain membrane regions and cytoskeleton of the parasites such as Toxoplasma gondii and other Apicomplexa [18]. MOPT and MORN3 were found to be expressed in the acrosomal region of elongating spermatids [19, 20], and a recent study suggested that MORN5 is both regulated by and required for BMP signaling during craniofacial development in chicken [14]. Based on these previous findings, we speculate that MORN5 may function in signaling during sperm development, and that it may participate in the tight interaction that occurs between the plasma membrane and cytoskeletal structures of a mature sperm flagellum.…”

Section: Discussionmentioning

confidence: 99%

Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice

et al. 2017

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The identification and characterization of germ cell-specific genes are essential if we hope to comprehensively understand the mechanisms of spermatogenesis and fertilization. Here, we searched the mouse UniGene databases and identified 13 novel genes as being putatively testis-specific or -predominant. Our in silico and in vitro analyses revealed that the expressions of these genes are testis- and germ cell-specific, and that they are regulated in a stage-specific manner during spermatogenesis. We generated antibodies against the proteins encoded by seven of the genes to facilitate their characterization in male germ cells. Immunoblotting and immunofluorescence analyses revealed that one of these proteins was expressed only in testicular germ cells, three were expressed in both testicular germ cells and testicular sperm, and the remaining three were expressed in sperm of the testicular stages and in mature sperm from the epididymis. Further analysis of the latter three proteins showed that they were all associated with cytoskeletal structures in the sperm flagellum. Among them, MORN5, which is predicted to contain three MORN motifs, is conserved between mouse and human sperm. In conclusion, we herein identify 13 authentic genes with male germ cell-specific expression, and provide comprehensive information about these genes and their encoded products. Our finding will facilitate future investigations into the functional roles of these novel genes in spermatogenesis and sperm functions.

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Section: Discussionmentioning

confidence: 99%

Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice

et al. 2017

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“…A Toxoplasma gondii protein that comprises repeated MORN motifs associates with structures that form the basal complex, the spindle pole and the apical complex, indicating that MORN motifs, through interaction with lipids, may be the linking membranous region to the cytoskeleton [29–31]. In addition, several MORN motif-containing proteins have been linked to a role in spermatozoa morphogenesis/biogenesis [32, 33]. A role of P. falciparum MORN motif-containing proteins in the regulation of the cytoskeleton is a definite possibility.…”

Section: Discussionmentioning

confidence: 99%

SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinase of the human malaria parasite Plasmodium falciparum

Abdi

Eschenlauer

Reininger

et al. 2010

Cell. Mol. Life Sci.

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Over the last decade, several protein kinases inhibitors have reached the market for cancer chemotherapy. The kinomes of pathogens represent potentially attractive targets in infectious diseases. The functions of the majority of protein kinases of Plasmodium falciparum, the parasitic protist responsible for the most virulent form of human malaria, remain unknown. Here we present a thorough characterisation of PfTKL3 (PF13_0258), an enzyme that belongs to the tyrosine kinase-like kinase (TKL) group. We demonstrate by reverse genetics that PfTKL3 is essential for asexual parasite proliferation in human erythrocytes. PfTKL3 is expressed in both asexual and gametocytes stages, and in the latter the protein co-localises with cytoskeleton microtubules. Recombinant PfTKL3 displays in vitro autophosphorylation activity and is able to phosphorylate exogenous substrates, and both activities are dramatically dependent on the presence of an N-terminal “sterile α-motif” domain. This study identifies PfTKL3 as a validated drug target amenable to high-throughput screening.Electronic supplementary materialThe online version of this article (doi:10.1007/s00018-010-0434-3) contains supplementary material, which is available to authorized users.

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“… 1 2 During the final stage, haploid round spermatids differentiate into spermatozoa with dramatic morphological changes, including nuclear condensation, acrosome and flagellum formation, and extrusion of residual cytoplasm to yield testicular spermatozoa. 3 4 5 Although the list of proteins specifically expressed in spermiogenesis has grown in recent years, many remain uncharacterized, and their roles await further investigation. 6 …”

Section: Introductionmentioning

confidence: 99%

Characterization of membrane occupation and recognition nexus repeat containing 3, meiosis expressed gene 1 binding partner, in mouse male germ cells

Zhang

Shang

et al. 2015

Asian J Androl

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Mammalian spermatogenesis is a well-organized process of cell development and differentiation. Meiosis expressed gene 1 (MEIG1) plays an essential role in the regulation of spermiogenesis. To explore potential mechanisms of MEIG1's action, a yeast two-hybrid screen was conducted, and several potential binding partners were identified; one of them was membrane occupation and recognition nexus repeat containing 3 (MORN3). MORN3 mRNA is only abundant in mouse testis. In the testis, Morn3 mRNA is highly expressed in the spermiogenesis stage. Specific anti-MORN3 polyclonal antibody was generated against N-terminus of the full-length MORN3 protein, and MORN3 expression and localization was examined in vitro and in vivo. In transfected Chinese hamster ovary cells, the antibody specifically crossed-reacted the full-length MORN3 protein, and immunofluorescence staining revealed that MORN3 was localized throughout the cytoplasm. Among multiple mouse tissues, about 25 kDa protein, was identified only in the testis. The protein was highly expressed after day 20 of birth. Immunofluorescence staining on mixed testicular cells isolated from adult wild-type mice demonstrated that MORN3 was expressed in the acrosome in germ cells throughout spermiogenesis. The protein was also present in the manchette of elongating spermatids. The total MORN3 expression and acrosome localization were not changed in the Meig 1-deficient mice. However, its expression in manchette was dramatically reduced in the mutant mice. Our studies suggest that MORN3 is another regulator for spermatogenesis, probably together with MEIG1.

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Identification and characterization of a novel mouse and human MOPT gene containing MORN-motif protein in testis

Cited by 12 publications

References 18 publications

Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice

Expression of uncharacterized male germ cell-specific genes and discovery of novel sperm-tail proteins in mice

SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinase of the human malaria parasite Plasmodium falciparum

Characterization of membrane occupation and recognition nexus repeat containing 3, meiosis expressed gene 1 binding partner, in mouse male germ cells

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